RESUMO
A selective, sensitive, rapid and reproducible high-performance liquid chromatographic method for the determination of terazosin in plasma is described. The structurally related compound prazosin was used as an internal standard. The method comprises extraction with methylene chloride followed by chromatography on a C18 reversed-phase column. The compounds were detected using spectrofluorimetry. The absolute recoveries were more than 90% with a minimal detection of 1 ng/ml and calibration curve was linear between 1 and 80 ng/ml.
Assuntos
Antagonistas Adrenérgicos alfa/sangue , Anti-Hipertensivos/sangue , Cromatografia Líquida de Alta Pressão/métodos , Prazosina/análogos & derivados , Antagonistas Adrenérgicos alfa/farmacocinética , Anti-Hipertensivos/farmacocinética , Fluorescência , Humanos , Prazosina/sangue , Prazosina/farmacocinética , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Fatores de TempoRESUMO
A rapid, reproducible high-performance liquid chromatographic method for the determination of secnidazole, 5-nitroimidazole class of antiprotozoals from blood is described. Metronidazole was used as an internal standard. A simple extraction step with dichloromethane was done before chromatography on a C18 column with the wavelength fixed at 276 nm on the UV detector. Blood levels up to 500 ng/ml have been measured with good precision in the healthy volunteers after 1 g of secnidazole was administered. The present described method can readily be utilized for routine pharmacokinetic studies.
