Exploring the optimization of aerobic food waste digestion efficiency through the engineering of functional biofilm Bio-carriers.

The possibility of breaking down cellulose-rich food waste through biofilm engineering was investigated. Six previously isolated strains from naturally degrading fruits and vegetables, screened for biofilm-forming ability and cellulolytic activity, were selected to enrich a biocarrier seeding microbial consortium. The food waste model used in this study was cabbage which was aerobically digested under repeated water rinsing and regular effluent drainage. The engineered biocarrier biofilm's functionality was evaluated by tracing microbial succession following metagenomic sequencing, quantitative PCR, scanning electron microscopy, and cellulolytic activity before and after the digestion processes. The engineered microbial consortium demonstrated superior biofilm-forming ability on biocarriers than the original microbial consortium and generally displayed a higher cellulolytic activity. The presented study provides one of the few studies of food waste aerobic digestion using engineered biofilms. Insights presented in this study could help further optimize aerobic food waste digestion.

Questioning the source of identified non-foodborne pathogens from food-contact wooden surfaces used in Hong Kong's urban wet markets.

In this study, a phylogenic analysis was performed on pathogens previously identified in Hong Kong wet markets' cutting boards. Phylogenetic comparisons were made between phylotypes obtained in this study and environmental and clinical phylotypes for establishing the possible origin of selected bacterial species isolated from wet market cutting board ecosystems. The results reveal a strong relationship between wet market bacterial assemblages and environmental and clinically relevant phylotypes. However, our poor knowledge of potential cross-contamination sources within these wet markets is further exacerbated by failing to determine the exact or presumed origin of its identified pathogens. In this study, several clinically relevant bacterial pathogens such as Klebsiella pneumoniae, Streptococcus suis and Streptococcus porcinus were linked to cutting boards associated with pork; Campylobacter fetus, Staphylococcus aureus, Escherichia coli, and A. caviae in those associated with poultry; and Streptococcus varanii, A. caviae, Vibrio fluvialis, and Vibrio parahaemolyticus in those associated with seafood. Identifying non-foodborne clinically relevant pathogens in wet market cutting boards in this study confirms the need for safety approaches for wet market meat, including cold storage. The presented study justifies the need for future systematic epidemiological studies to determine identified microbial pathogens. Such studies should bring about significant improvements in the management of hygienic practices in Hong Kong's wet markets and work towards a One Health goal by recognizing the importance of wet markets as areas interconnecting food processing with animal and clinical environments.

Simultaneous removal of pollutants from water using nanoparticles: A shift from single pollutant control to multiple pollutant control.

The steady increase in population, coupled with the rapid utilization of resources and continuous development of industry and agriculture has led to excess amounts of wastewater with changes in its composition, texture, complexity and toxicity due to the diverse range of pollutants being present in wastewater. The challenges faced by wastewater treatment today are mainly with the complexity of the wastewater as it complicates treatment processes by requiring a combination of technologies, thus resulting in longer treatment times and higher operational costs. Nanotechnology opens up a novel platform that is free from secondary pollution, inexpensive and an effective way to simultaneously remove multiple pollutants from wastewater. Currently, there are a number of studies that have presented a myriad of multi-purpose/multifunctional nanoparticles that simultaneously remove multiple pollutants in water. However, these studies have not been collated to review the direction that nanoparticle assisted wastewater treatment is heading towards. Hence, this critical review explores the feasibility and efficiency of simultaneous removal of co-existing/multiple pollutants in water using nanomaterials. The discussion begins with an introduction of different classes of pollutants and their toxicity followed by an overview and highlights of current research on multipollutant control in water using different nanomaterials as adsorbents, photocatalysts, disinfectants and microbicides. The analysis is concluded with a look at the current attempts being made towards commercialization of multipollutant control/multifunctional nanotechnology inventions. The review presents evidence of simultaneous removal of pathogenic microorganisms, inorganic and organic compound chemical pollutants using nanoparticles. Accordingly, not only is nanotechnology showcased as a promising and an environmentally-friendly way to solve the limitations of current and conventional centralised water and wastewater treatment facilities but is also presented as a good substitute or supplement in areas without those facilities.

Microbial cell immobilization in biohydrogen production: a short overview.

The high dependence on fossil fuels has escalated the challenges of greenhouse gas emissions and energy security. Biohydrogen is projected as a future alternative energy as a result of its non-polluting characteristics, high energy content (122 kJ/g), and economic feasibility. However, its industrial production has been hampered by several constraints such as low process yields and the formation of biohydrogen-competing reactions. This necessitates the search for other novel strategies to overcome this problem. Cell immobilization technology has been in existence for many decades and is widely used in various processes such as wastewater treatment, food technology, and pharmaceutical industry. In recent years, this technology has caught the attention of many researchers within the biohydrogen production field owing to its merits such as enhanced process yields, reduced microbial contamination, and improved homogeneity. In addition, the use of immobilization in biohydrogen production prevents washout of microbes, stabilizes the pH of the medium, and extends microbial activity during continuous processes. In this short review, an insight into the potential of cell immobilization is presented. A few immobilization techniques such as entrapment, adsorption, encapsulation, and synthetic polymers are discussed. In addition, the effects of process conditions on the performance of immobilized microbial cells during biohydrogen production are discussed. Finally, the review concludes with suggestions on improvement of cell immobilization technologies in biohydrogen production.