The Evaluation of Biomarkers of Physical Activity on Stress Resistance and Wellness.

Physical activity can improve health as well as reduce stress and the risk of developing several widespread diseases. However, there exists no accepted standard biomedical examination-method for stress evaluation. The purpose of this study was to investigate the effect of regular physical activity on stress and wellness as well as the evaluation of potential biomarkers in this field. This study included 105 people (mean age = 36.57 ± 1.4 years) who were randomly assigned into the exercise group 1 (EG-1) (n = 41), the exercise group 2 (EG-2) (n = 30), and the control group (CG) (n = 34). Measurements of stress and wellness were obtained by Multiscan BC-OXI before and after experimental period. This device presents a multifrequency segmental body composition 3D analyser with digital pulse oximeter. The key indicators of stress as well as for wellness were significantly improved in the EG-1. Parasympathetic activity showed significant changes as potential stress biomarker. Statistically significant gender differences were not observed in the comparable groups. The results suggest that the stress resistance and well-being significantly improved in the EG-1 due to regular physical activity. However, further research is necessary to determine effects of physical activity on integral health indicators.

Peculiarities of the anticoagulant properties for the newly synthesized preparations of coumarin-related compounds.

A) OBJECTIVES: Investigations of the four synthetic coumarin-related compounds: N' - allylthiouridine-3-carbamoil coumarin (GSH-16), N'- morpholylthiouridine-3-carbamoil coumarin (GSH-17), N'-O-fluor-benzyl-N'-3-carbamoil piperaside coumarin (GSH-10) and 6-nitroallylamide-3-carboxy coumarin (GSH-84), were done for study of their role in the processes of hemocoagulation. B) DESIGN AND METHODS: Investigations were carried out on 120 white rats (180-200g), which were injected intravenously by 0.5 ml and 1.0 ml of 1 % solution of GSH-17 and decapitated after 10 and 30 min after the injection by use of light chloroform narcosis. Separation of the liver was done by simultaneous washing with cold physiological solution. C) RESULTS: It was shown that the hemostabilization action of GSH-17 is highly dose- and time - dependent, with by the pronounced decrease of rat liver thromboplastic activity after 10 and especially 30 min following intravenous injection. D) CONCLUSION: One of the possible mechanisms for haemostatic effect of the studied preparations, particularly GSH-17, probably can be accepted their effect on metabolism of arachidonic acid by lipoxygenase and cycloxygenase mechanisms [9, 10]. The results of this investigations have not only the academic interest, but they have also a significant importance for definite branches of practical medicine as a very effective blood stabilizing factors.