[Determination of bacterial endotoxin admixtures in inactivated influenza vaccines]. / Opredelenie primesei bakterial'nykh éndotoksinov v inaktivirovannykh grippoznykh vaktsinakh.

The materials substantiating the possibility of using the method for the determination of the lethal effect of endotoxin on dactinomycin-treated mice are presented. This determination is made with a view to detecting the admixtures of endotoxins in whole-virion and subvirion inactivated influenza vaccines at different stages of their manufacture, as well as in the final product. The proposed test is highly sensitive, rather simple in its practical realization and can be used for evaluating the degree of the purification of influenza vaccines from endotoxins.

[Postvaccinal dysentery immunity studied on the model of ligated loop of the small intestine of rabbits]. / Postvaktsinal'nyi protivodizenteriinyi immunitet na modeli pereviazannoi petli tonkoi kishki krolikov.

Chemical (detergent) antigens administered intraintestinally or intravenously to rabbits were applied to reproduce postvaccinal immunity to experimental shigellosis. There was noted an increase of the antibody level in the serum, an intensification of its preventive proerties, and the absence of dilatation of the isolated loops of the small intestine after the administration of the corresponding dysentery culture. Oral immunization of rabbits with the detergent antigen sorbed on ionexchange resin also led to immunity.

[Study of the immunological effectiveness of the enteral vaccination of volunteers with detergent Sonne dysentery monovaccine]. / Izuchenie immunologicheskoi éffektivnosti énteral'noi vaktsinatsii dobrovol'tsev dizenteriinoi detergentnoi monovaktsinoi Zonne.

The authors evaluated the immunological results of enteral and subcutaneous injection of detergent Sonne dysentery vaccine to volunteers (changes in homo- and heterologous antibodies in the blood and saliva recorded by means of common indirect hemagglutination test and that modified according to Coombs' test, and IgG, IgM, and IgA concentration recording. Both immunization methods induced specific antibody formation: enteral vaccination caused chiefly the accumulation of secretory antibodies of IgA class, and subcutaneous -- of serum antibodies belonging to IgG class. The supposed mechanisms determining the capacity of enteral administration of the antigen to provoke an immune response different from that caused by its subcutaneous injection are discussed.

[Reactivity and immunogenicity of the Sonne dysentery detergent monovaccine]. / Reaktogennost' i immunogennost' dizenteriinoi detergentnoi monovaktsiny Zonne

The authors elaborated a method of isolation under industrial conditions of Shigella sonnei surface antigens by the treatment of the culture with a detergent (sodium laurylsulfate). The antigens obtained possessed a much lesser toxicity in comparison with the tryptic ones. A method of preparation of a detergent Sonne monovaccine with an increased antigen content (1.3 mg/ml) was described. Experimental series of the preparation were tested on 38 volunteers. A lower reactogenicity of the detergent vaccine (in comparison with the tryptic) and its marked immunological efficacy was demonstrated.