Subcortical profile of memory compromise among HIV-1-infected individuals.

OBJECTIVE: To examine the hypothesis of subcortical pathologic impairment in HIV/AIDS. METHOD: The study included 22 HIV+, 22 HIV- controls, 22 learning disordered (LD) HIV-, and 22 depressive HIV-. The groups were compared on eight WMS-III Indices. RESULTS: Analyses revealed significantly lower scores (p < .05) in HIV+ on visual immediate memory, immediate memory, visual delayed memory, auditory delayed memory, working memory, and general memory. For all cases, HIV+ participants scored below the control group only. CONCLUSIONS: WMS-III indices discriminated HIV+ participants from normal comparisons. Inability to find differences between HIV+ and depressive and LD groups reflects the isolation of the subcortical effect to the HIV+ group.

Ground based radon-222 observations and their application to atmospheric studies.

The aim of this paper is to review recent trends in the application of ground based radon observations to atmospheric research. In spite of over four decades of atmospheric radon monitoring, only in the past decade has the potential of this passive tracer been realised through a series of atmospheric model evaluation studies. Firstly, the key operational requirements for baseline radon detectors are briefly discussed, including lower limit of detection and response time. Then, current radon-related benchmarks for the evaluation of regional and global models are reviewed, with particular consideration given to the implications of data availability, resolution, site location and model spatial/temporal resolution. An 8-year subset of radon observations from the Cape Grim Baseline Air Pollution Station is used to suggest new benchmarks that exploit long-term data sets. Lastly an overview is presented of a technique that uses radon to estimate regional fluxes of climatically sensitive gases, with specific examples for CO2, CH4 and N2O.

COWAT metanorms across age, education, and gender.

Norms for the Controlled Oral Word Association Test (COWAT; Benton & Hamsher, 1976) have been published as summary statistics from many relatively small samples. The purpose of this study was to combine statistics from these samples to produce metanorms broken down by age, gender, and level of education. Means and standard deviations of letters F, A, and S (FAS) totals from normative samples and samples of normal controls were gathered from a wide variety of research studies and then stringently selected based on factors such as adequacy of the sample and presence of the appropriate statistics. The resulting aggregate statistics for FAS totals suggest differences may exist in verbal fluency task performance, depending on a person 's age, gender, and level of education.

The detection of malingered posttraumatic stress disorder with MMPI-2 fake bad indices.

This investigation explored the effect of posttraumatic stress disorder (PTSD) simulation on Minnesota Multiphasic Personality Inventory-2 (MMPI-2) responses, to detect malingered from genuine PTSD. Sixty-four adult PTSD outpatients at a child sexual abuse (CSA) survivor treatment program were compared with 85 adult college students instructed and trained to malinger PTSD. MMPI-2 overreporting indices examined were F, F-Fb, F-K, F(p), Ds2, O-S, OT, and FBS. A stepwise discriminant analysis identified F(p), F-K, and O-S as the best malingering predictors. A predictive discriminant analysis yielded good hit rates for the model, with impressive cross-validation results. Cutoff scores were assessed for the model's predictors. Clinical implications for detecting malingered PTSD using the MMPI-2 are discussed.

Assessment of visual-motor coordination in 6- to 11-yr.-olds.

The concurrent and content validity of the Rey-Osterrieth Complex Figure Test and the Beery Developmental Test of Visual-Motor Integration-Third Revision were investigated through correlational analysis. 432 children, ages 6 to 11 years, were administered both tests. Across age groups participants performed better on both measures, providing support for the similarity of the measures and their sensitivity to the development of visuomotor integration. Although analysis indicated considerable overlap in the content of the two scales, the shared variance ranged from 7% to 31%, depending on the age of the child. The Rey-Osterrieth figure is composed of overlapping squares, rectangles, triangles, and various other shapes. Given this complex combination, scores on this test reflect the examinees' visual organization and motor planning skills. On the other hand, the Beery test consists of a series of shapes which progress from simple figures to more complex ones. Because the figures become more difficult to copy, the score on this test reflects the examinees' developmental level of visuomotor ability. Despite these differences in test stimuli and interpretation of performance, the present study showed considerable shared variance in the scores of the examinees who took both tests. Finally, local norms for the Rey-Osterrieth figure using the scoring approach of E. M. Taylor (1959, adapted from Osterrieth, 1944) and including standard scores are presented for children ages 6 to 11 years.

A comparison of psychological/psychiatric symptomatology of women and men sexually abused as children.

OBJECTIVE: The purpose of this study was to explore gender differences in symptomatology among sexual abuse survivors utilizing a standardized measure of specific symptom patterns, the Symptom Checklist 90-Revised (SCL-90-R). METHOD: Gender differences in symptomatology of adults sexually victimized as children were examined. Participants were 162 women and 25 men entering an outpatient treatment program for adult survivors of childhood sexual abuse (CSA) in a university-based community mental health center. Symptomatology was measured using the Symptom Checklist 90-Revised (SCL-90-R). RESULTS: Although no differences appeared when examining the raw data, the results changed dramatically once the data were converted into T-scores and epidemiological SCL-90-R gender differences were taken into account. The findings indicate that men exhibited significantly more interpersonal sensitivity, depression, anxiety, and phobic anxiety than women in relation to their respective normative samples. CONCLUSIONS: The use of nonclinical T-scores in this study allows for the interpretation that men survivors of childhood sexual abuse (CSA) have higher levels of symptomatology than women survivors when compared to their respective normative samples.

Advancing from flexible sigmoidoscopy to colonoscopy in rural family practice.

This paper describes the background, experience, training, and preceptorship of a rural family physician that culminated in provision of diagnostic and therapeutic colonoscopy to his patients. Initial training took place in a two-day continuing medical education course. Subsequent training consisted of one-on-one training in 11 colonoscopies and five polypectomies, correspondence, recommended readings, a one-on-one preceptorship, and telephone consultation. Training was provided by University of Tennessee faculty who were experts in the area of colonoscopy and polypectomy procedures. The outcomes of 250 consecutive colonoscopies performed by the rural family physician are documented here. Training requirements vary widely by professional organization and subspecialty. Some subspecialists have recommended as many as 100 supervised colonoscopies and 25 polypectomies as a minimum training requirement for hospital privileges. It is our contention that unnecessarily high training requirements add to educational costs and may restrict qualified rural physicians from providing these services.

Modeling the Exchanges of Energy, Water, and Carbon Between Continents and the Atmosphere

Atmospheric general circulation models used for climate simulation and weather forecasting require the fluxes of radiation, heat, water vapor, and momentum across the land-atmosphere interface to be specified. These fluxes are calculated by submodels called land surface parameterizations. Over the last 20 years, these parameterizations have evolved from simple, unrealistic schemes into credible representations of the global soil-vegetation-atmosphere transfer system as advances in plant physiological and hydrological research, advances in satellite data interpretation, and the results of large-scale field experiments have been exploited. Some modern schemes incorporate biogeochemical and ecological knowledge and, when coupled with advanced climate and ocean models, will be capable of modeling the biological and physical responses of the Earth system to global change, for example, increasing atmospheric carbon dioxide.

Isolated sulfite oxidase deficiency.

Isolated sulfite oxidase (SO) deficiency is an autosomal recessively inherited inborn error of sulfur metabolism. In this report of a ninth patient the clinical history, laboratory results, neuropathological findings and a mutation in the sulfite oxidase gene are described. The data from this patient and previously published patients with isolated sulfite oxidase deficiency and molybdenum cofactor deficiency are summarized to characterize this rare disorder. The patient presented neonatally with intractable seizures and did not progress developmentally beyond the neonatal stage. Dislocated lenses were apparent at 2 months. There was increased urine excretion of sulfite and S-sulfocysteine and a decreased concentration of plasma cystine. A lactic acidemia was present for 6 months. Liver sulfite oxidase activity was not detectable but xanthine dehydrogenase activity was normal. The boy died of respiratory failure at 32 months. Neuropathological findings of cortical necrosis and extensive cavitating leukoencephalopathy were reminiscent of those seen in severe perinatal asphyxia suggesting an etiology of energy deficiency. A point mutation that resulted in a truncated protein missing the molybdenum-binding site has been identified.

Prediction of premorbid intellectual functioning of young children using demographic information.

Regression equations were developed for the estimation of Wechsler Preschool and Primary Scale of Intelligence - Revised (WPPSI-R) Verbal, Performance and Full Scale IQs from the demographic characteristics of the WPPSI-R standardization sample (N=1700) Parent's education level, child's ethnicity, parent's occupation, and geographic region were found to be most predictive of the IQs The three resultant models produced statistically significant Rs of 0 54, 0 52, and 0 44 for Full, Verbal and Performance scales, respectively All three models showed minimal shrinkage upon crossvalidation, but results of tests for similarity of magnitude, variation, and classification were considerably poorer Guidelines and cautions for the clinical use of these models are discussed Also, the results are discussed in the context of similar studies.

A new family of outwardly rectifying potassium channel proteins with two pore domains in tandem.

Potassium channels catalyse the permeation of K+ ions across cellular membranes and are identified by a common structural motif, a highly conserved signature sequence of eight amino acids in the P domain of each channel's pore-forming alpha-subunit. Here we describe a novel K+ channel (TOK1) from Saccharomyces cerevisiae that contains two P domains within one continuous polypeptide. Xenopus laevis oocytes expressing the channel exhibit a unique, outwardly rectifying, K(+)-selective current. The channel is permeable to outward flow of ions at membrane potentials above the K+ equilibrium potential; its conduction-voltage relationship is thus sensitive to extracellular K+ ion concentration. In excised membrane patches, external divalent cations block the channel in a voltage-dependent manner, and their removal in this configuration allows inward channel current. These attributes are similar to those described for inwardly rectifying K+ channels, but in the opposite direction, a previously unrecognized channel behaviour. Our results identify a new class of K+ channel which is distinctive in both its primary structure and functional properties. Structural homologues of the channel are present in the genome of Caenorhabditis elegans.

Approaches to memory assessment in the chronic psychiatric elderly.

Approaches to memory assessment generally fall into two categories: a) Evaluation of the integrity of specific cognitive processes, and b) Quantitative measurement of performance levels on global dimensions of behavior. The California Verbal Learning Test (CVLT) is an exemplar of both the process and global approaches, while the Wechsler Memory Scale-Revised (WMS-R) represents the global approach. Despite the popularity of both instruments little extant research, examining their convergent/divergent properties or their compatibility, exists. In the present study we replicated, on a mixed sample of normal and psychiatric elderly, a previous study's pattern of CVLT/WMS-R convergent and divergent findings. In addition, we found that both instruments are similar in their discrimination between normal and psychiatric elderly subjects.

Base rates for the Selz-Reitan. Index of subtest scatter on the WPPSI-R.

Using the standardization sample of the Wechsler Preschool and Primary Scale of Intelligence-Revised (WPPSI-R) an analysis was done to determine the base rates of occurrence of the Selz-Reitan Index (SRI) of unusual or abnormal scatter. Findings offered support for the use of the WPPSI-R to determine unusually or abnormally large SRIs in clinical practice. However, It was found that the clinical interpretation of obtained indexes depend upon the Full Scale IQ of the individual and the level of the SRI selected for interpretation. Further research on clinical neurological populations is encouraged.

Activation of BKCa channels in acutely dissociated neurones from the rat ventromedial hypothalamus by NS 1619.

NS 1619 activates the large-conductance Ca(2+)-dependent K+ channel (BKCa) in membrane patches isolated from rat ventromedial hypothalamic neurones. The activation is concentration dependent, with a maximal effect at less than 30 microM, reversible and can be inhibited by application of iberiotoxin to the extracellular membrane. NS 1619 does not activate ATP-K+ channels present in the same neurones.

Lack of effect of potassium channel openers on ATP-modulated potassium channels recorded from rat ventromedial hypothalamic neurones.

1. Single neuronal cells were freshly isolated from the ventromedial hypothalamic nuclei (VMHN) of the rat brain. Currents through ATP-modulated and large conductance (160 and 250 pS) calcium-activated potassium channels were recorded by the cell-attached and excised inside-out patch techniques. 2. BRL38227 (lemakalim; 30-90 microM) applied to the superfusing medium produced no change in firing rate of isolated glucose-receptive VMHN neurones in cell-attached recordings. 3. BRL38227, at concentrations of between 30-100 microM applied to the intracellular (cytoplasmic) aspect of inside-out patches, had no effect on the activity of ATP-sensitive K+ channels in the absence of ATP or in the presence of a sub-maximal inhibitory concentration (3 mM) of ATP. Cromakalim, pinacidil, minoxidil sulphate and diazoxide also produced no effect under these conditions. 4. The potassium channel openers (KCO's) were tested on ATP-activated potassium channels recorded from a further subpopulation of VMHN neurones. Application of BRL38227 (up to and including 100 microM) to this channel in inside-out patches either in the absence of ATP or when activated by 5 mM ATP had no effect on channel activity. Identical results were obtained with cromakalim and pinacidil. 5. BRL38227 had no effect on either of the large conductance (250 pS and 160 pS) calcium-activated potassium channels in VMHN neurones. 6. Intracellular recordings were made from glucose-receptive VMHN neurones in rat brain slices. Cromakalim (50 microM) or diazoxide (60 microM) did not alter the firing rate or passive membrane properties of these neurones demonstrated to be sensitive to tolbutamide (0.1 mM). 7. These results show that the KCO's tested in this study have no effect either on VMHN neurones contained in brain slices or on the activity of any of the ATP-modulated potassium channels under isolated patch conditions associated with these neurones.

Phosphinic acid analogues of GABA are antagonists at the GABAB receptor in the rat anococcygeus.

CGP 35348 (3-aminopropyl(diethoxymethyl)phosphinic acid) and 3-aminopropyl(n-hexyl)phosphinic acid (3-APHPA) were tested in the rat anococcygeus muscle against CGP 27492 (3-aminopropylphosphinic acid), a selective GABAB agonist, for their antagonist activity. Their antagonist potency was compared with that of 2-hydroxysaclofen. The pA2 values for CGP 35348, 3-APHPA and 2-hydroxysaclofen were 5.38, 4.86, 4.45 respectively in the rat anococcygeus muscle. These results confirm the previous reports of GABAB antagonist activity for these compounds and show a marginal improvement in potency over 2-hydroxysaclofen.

Modelling and monitoring 'Greenhouse' warming.

Greenhouse warming is now commonly accepted by politicians and the general public and is the subject of active research. This paper traces the greenhouse theory as far back as 1827, highlighting new directions and significant advances over that time. Three main themes emerge: that certain radiatively active gases are responsible for warming the planet; that humans are increasing the concentrations of these gases and hence inadvertently influencing this warming; that climate models are designed to permit prediction of the climatic changes resulting from changed loadings in these gases, but that they have not yet achieved this goal of prediction.

An apparatus to retain the spatial orientation of breast biopsies.

An apparatus has been designed to retain the spatial orientation of breast biopsies throughout surgery, transport and processing in the pathology laboratory. It does not interfere with regular surgical procedures and simplifies the surgeon's task of marking the orientation of a specimen. As the use of the apparatus does not involve either chemical or physical substances it is an appropriate handling procedure for those specimens requiring sensitive testing procedures such as cell culture.