RESUMO
An attenuated Campylobacter jejuni aspartate chemoreceptor ccaA mutant caused gross pathological changes despite reduced colonisation ability in animal models. In chickens, the pathological changes included connective tissue and thickening of the mesenteric fat, as well as the disintegration of the villus tips in the large intestine, whereas in mice, hepatomegaly occurred between 48-72 hours post infection and persisted for the six days of the time course. In addition, there was a significant change in the levels of IL-12p70 in mice infected with the C. jejuni ccaA mutant. CcaA isogenic mutant was hyper-invasive in cell culture and microscopic examination revealed that it had a "run" bias in its "run-and-tumble" chemotactic behaviour. The mutant cells also exhibited lower level of binding to fucosylated and higher binding to sialylated glycan structures in glycan array analysis. This study highlights the importance of investigating phenotypic changes in C. jejuni, as we have shown that specific mutants can cause pathological changes in the host, despite reduction in colonisation potential.
Assuntos
Ácido Aspártico/metabolismo , Campylobacter jejuni/genética , Campylobacter jejuni/metabolismo , Animais , Ácido Aspártico/genética , Proteínas de Bactérias/metabolismo , Infecções por Campylobacter/veterinária , Campylobacter jejuni/patogenicidade , Células Quimiorreceptoras/metabolismo , Galinhas/metabolismo , Modelos Animais de Doenças , Interações Hospedeiro-Patógeno , Inflamação , Mucosa Intestinal/metabolismo , Masculino , Camundongos , Camundongos EndogâmicosAssuntos
Doenças Renais Císticas/diagnóstico por imagem , Neoplasias Renais/diagnóstico por imagem , Adulto , Idoso , Angiografia , Reações Falso-Negativas , Humanos , Doenças Renais Císticas/diagnóstico , Neoplasias Renais/irrigação sanguínea , Neoplasias Renais/diagnóstico , Pessoa de Meia-Idade , Tomografia Computadorizada por Raios X , Ultrassonografia , UrografiaAssuntos
Hipertensão Renovascular/diagnóstico por imagem , Processamento de Imagem Assistida por Computador/métodos , Neoplasias Renais/diagnóstico por imagem , Artéria Renal/diagnóstico por imagem , Técnica de Subtração , Adolescente , Adulto , Idoso , Criança , Meios de Contraste , Diatrizoato de Meglumina , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Radiografia , Obstrução da Artéria Renal/diagnóstico por imagemRESUMO
The authors consider the potentialities of computerized tomography (CT) in the diagnosis of lesions of the retroperitoneal lymph nodes in their metastatic involvement and hemoblastoses. CT data on 142 patients with hemoblastoses and tumors of different sites are discussed. This type of examination made it possible to reveal the involvement of the retroperitoneal lymph nodes in 59 patients resulting in the diagnosis of the generalization of the underlying disease in 41.5% of the cases. CT makes it possible to correctly interpret the state of practically all groups of the retroperitoneal lymph nodes. Besides, CT gives an opportunity to detect lymph node enlargement in the negative results of lymphography or to reveal lesions of some groups of the lymph nodes which are inaccessible to contrast examination, all of these being of great importance for the planning of radiation or surgical treatment. CT provides an opportunity for a dynamic control over therapeutic results both in hospitals and in polyclinics. The results of the examination were of importance in each case for the choice of the tactics and method of specific therapy as well as for prognosis of a subsequent source of the underlying disease.
