[Use of Pseudomonas and Achromobacter species bacteria--degraders of surface-active agents--for detection and destruction of polycyclic aromatic hydrocarbons]. / Ispol'zovanie bakterii-destruktorov poverkhnostno-aktivnykh veshchestv rodov Pseudomonas i Achromobacter dlia detektirovaniia i destruktsii politsiklicheskikh aromaticheskikh uglevodorodov.

The developed biosensor models were based on the use of immobilized Pseudomonas and Achromobacter cells for polycyclic aromatic hydrocarbons and surfactants detection. The responses of biosensors based on bacteria-degraders of anionic surfactants for organic substrates, which related to different classes of surfactants, aromatic and policyclic aromatic hydrocarbons (PAH) were investigated. The sensor showed the highest sensitivity to anionic surfactants and PAH. The lower limit of sodium dodecyl sulfate detection is within a range of 0.25-0.5 mg/l (0.86-1.73 microM). The sensors showed the highest sensitivity to naphthalene (1-6 mM) and anthracene, fluorene, phenanthrene. All strains that have been investigated may be used as a receptor element of biosensors for detection of PAH and surfactants.

[Effect of conditions of culturing Pseudomonas rathonis T on characteristics of a biosensor for determining anionic surface-active agents]. / Vliianie uslovii kul'tivirovaniia Pseudomonas rathonis T na kharakteristiki biosensora dlia opredeleniia anionnykh poverkhnostno-aktivnostno-aktivnykh veshchestv.

The dependence of the sensitivity of a microbial biosensor of anionic surfactants (AS) on the growth phase of Pseudomonas rathonis T, a strain capable of degrading surfactants, was studied. Correlations were found between the optimum values of temperature and pH of microbial growth, substrate utilization, and functional performance of the microbial biosensor. These results allow the process of AS detection to be optimized.

[Effect of various methods of immobilization on stability of a microbial biosensor based on Pseudomonas rathonis T during detection of surfactants]. / Vliianie razlichnykh sposobov immobilizatsii na stabil'nost' mikrobnogo biosensora na osnove Pseudomonas rathonis T pri detektsii poverkhnostno-aktivnykh veshchestv.

The operating and storage stability of a receptor element of an amperometric biosensor based on the Pseudomonas rathonis strain T capable of degrading surfactants was tested. Microbial cells were immobilized by incorporation in gels (agar, agarose, and calcium-alginate), polyvinyl alcohol membrane, adhesion to the chromatographic paper GF/A, or by the cross-linking induced by glutaric aldehyde. Incorporation of microbial cells in agar gel provides the long-standing conservation of their activity and viability during measurements of high concentrations of surfactants and allows the receptory element of the biosensor to be rapidly recovered after the measurements.