RESUMO
In this article, we present the first case report of a chicken mycosis caused by F. proliferatum occurred on a private farm in the Russian Federation. Lesions on the skin of the legs and scallops were reported. The object of this study was samples of feed and pathological material from sick hens-layers. Mycological analysis included determination of the total number of fungi (TNF) and identification and determination of the toxicity and pathogenicity of the isolates. The identification of the isolate was carried out taking into account direct microscopy, morphological features, and the method of molecular genetic analysis. Microscopic fungi of the genus Penicillium and Rhizopus were isolated by mycological analysis of the feed. The test feed was nontoxic. Mycological examination of pathological material (scrapings from the combs and affected legs) identified an isolate of Fusarium proliferatum, which showed toxicity on biological objects (protozoa, rabbits) and pathogenicity (white mice). Dermal application of F. proliferatum suspension was accompanied by reddening of the rabbit skin. Intraperitoneal injection of fungal spores caused mycosis in white mice. Polymerase chain reaction (PCR) made it possible to identify this type of microscopic fungus (F. proliferatum) with high accuracy in the samples under study. The research results allow us to consider F. proliferatum as a cause of poultry disease against the background of predisposing factors in the form of desquamation of the stratum corneum of the skin against the background of immunosuppression and metabolic disorders caused by an imbalance in the diet.
RESUMO
The aflatoxin B1 (AFB1) adsorption properties of several lignins isolated by the Pepper's method from grassy plants: Helianthus tuberosus (LS-1), Atriplex patula (LS-2), Rhododendron tomentosum (LS-3), Althaea officinalis (LS-4), Lavatera (LS-5), and from wood of spruce Pícea (LS-6) were studied. The adsorption was conducted in vitro in the modeled conditions of the gastrointestinal tract. The correlations between the values of adsorption-desorption and the parameters of surface-porous structure and chemical structure of the lignins were established. The relationships between the adsorption capacity and properties of the lignins led us to the conclusion that chemisorption play the most important role in the strong adsorption of aflatoxin B1. The contribution of the surface physical properties to the process of adsorption of aflotoxin B1 was not significant. It was shown that the sample of lignin isolated from stems of Althaea officinalis, was characterized by the highest value of strong adsorption of aflatoxin B1.
