A novel impedimetric sensor based on N-acetyl-L-cysteine for the determination of hydroxyl radicals in cell cultures in vitro.

We report a novel impedimetric sensor based on a graphite electrode impregnated with polyethylene and paraffin under vacuum (IGE) modified with electrochemically deposited gold and a self-assembled monolayer of N-acetyl-L-cysteine (NAC/Au/IGE) for selective and sensitive determination of extracellular hydroxyl radicals (OH•) generated by living cells. The application of a sulphur-containing molecule oxidized by OH• predicts the high selectivity of the sensor, and the utilization of the non-faradaic impedance spectroscopy for recording an analytical response makes it possible to achieve superior sensitivity with a detection limit of 0.01 nM and a linear dynamic range of 0.08-8 nM. Meanwhile, NAC/Au/IGE demonstrated a strong potential of detecting OH• generated by biological objects via successful determination of extracellular hydroxyl radicals generated by normal fibroblast cells and prostate carcinoma cells.

[Consumption of carbohydratecontaining beverages and their contribution to the total calorie content of the diet].

The article presents data on the frequency of consumption of carbohydrate-containing sweet carbonated drinks by the population of the Russian Federation and their contribution to the overall caloric intake of the diet. Questioning 11 850 people of different ages (from 12 to 60 years) and sex in all eight Federal Districts of Russia has been conducted. The frequency of food consumption has been studied, and in parallel dietary intake has been assessed using 24-hour recall method. The survey showed a fairly low frequency of consumption of sweet carbonated beverages and no significant differences in the frequency of their consumption by the population of various federal districts of Russia. With a certain frequency from 55.5 to 67.3% of the population consumed sweet carbonated drinks, while 18.1-20.9% of the respondents did not consume them more often 1-3 times a month, and 1.3% of the surveyed (from 0.3% in the North- West to 3.9% in the Southern Federal District) - 2 times a day or more often. Analysis of the data obtained showed that the contribution of the carbohydrate component contained in sweet carbonated beverages, even when consumed frequently (5-6 times a week) did not exceed 3.71% of the total diet calorie intake and not more than 7.1% of the caloric value of carbohydrates' intake.

Stochastic wave-function unravelling of the generalized Lindblad equation.

We investigate generalized non-Markovian stochastic Schrödinger equations (SSEs), driven by a multidimensional counting process and multidimensional Brownian motion introduced by A. Barchielli and C. Pellegrini [J. Math. Phys. 51, 112104 (2010)JMAPAQ0022-248810.1063/1.3514539]. We show that these SSEs can be translated in a nonlinear form, which can be efficiently simulated. The simulation is illustrated by the model of a two-level system in a structured bath, and the results of the simulations are compared with the exact solution of the generalized master equation.

[Frequency of consumption of sweet carbonated drinks by the population of different age groups of the Russian Federation].

The article analyzes the frequency of consumption of sweet carbonated beverages by various age groups (from 12 to 60 years) of the population of all Federal districts of Russian Federation. The presence of general regularities in the frequency of consumption of these drinks, regardless of the region, was established. About 5-8% of the population of different age and sex groups consumed sweet carbonated drinks with a fairly high frequency (5-6 times a week). The analysis showed that there was no dependence of the body mass index on the frequency of consumption of carbonated beverages and the absence of gender and age differences.

[The use of contact thermography for evaluating joint inflammation in rheumatoid arthritis]. / Primenenie kontaktnoi termografii dlia otsenki vospaleniia sustavov pri revmatoidnom artrite.

The paper is concerned with the results of a study of the hand joints in patients with different variants of a course and activity of rheumatoid arthritis by a method of contact liquid crystal thermography. Methods for assessment of thermography taking account of qualitative and quantitative indices were described. They showed correlation with a degree of disease activity, the presence of the rheumatoid factor, extraarticular symptoms and a time-period. Probable pathogenetic mechanisms of occurrence of characteristic thermographic shifts in rheumatoid arthritic patients were discussed.