RESUMO
In the course of the work done the specific and quantitative composition of the streptococcal autoflora of the fauces of the cosmonauts and the members of backup drew was investigated. In populations of isolated microorganisms the non-pathogenic streptococci have dominated among which S. salivarius prevailed. The same species has constantly been isolated in all the cosmonauts, pre- and postflight. Observation of the microflora state of the fauces at different stages of their professional activity made it possible to reveal the peculiarities of an individual dynamics in the number of nonpathogenic streptococci isolated from tampon depending on the participation of the test-subjects in the previous space missions. This is evidently a reflection of the effect of psychoemotional tension on the state of colonization resistance (CR) of the fauces mucosa and, as result of this, on its microflora. In turn, the occurrence of the individual species of conditionally-pathogenic streptococci after the mission points to a decrease in the CR under effect of unfavourable factors of space mission.
Assuntos
Astronautas , Boca/microbiologia , Faringe/microbiologia , Streptococcus/isolamento & purificação , Seguimentos , Humanos , Nariz/microbiologia , Voo Espacial , Streptococcus/patogenicidadeRESUMO
The microbiological monitoring of obstetric institutions revealed essential changes in the microbiological structure of hospital purulent septic infections in newborns and parturient women during the period of 1987-1992. An increase in the role of gram-negative microflora, mainly the representatives of the family Enterobacteriaceae, was registered. The specific proportion of gram-negative microorganisms in the etiology of hospital infections increased from 43.7% to 95.1% in newborn infants and from 33.3% to 61.3% in parturient women, which differently affected on the structure of the main nosological forms of these diseases.
Assuntos
Infecção Hospitalar/microbiologia , Infecção Puerperal/microbiologia , Sepse/microbiologia , Adulto , Infecção Hospitalar/etiologia , Feminino , Infecções por Bactérias Gram-Negativas/etiologia , Infecções por Bactérias Gram-Negativas/microbiologia , Maternidades , Humanos , Recém-Nascido , Lituânia , Infecção Puerperal/etiologia , Federação Russa , Sepse/etiologiaAssuntos
Doenças Transmissíveis/diagnóstico , Portador Sadio/diagnóstico , Portador Sadio/epidemiologia , Portador Sadio/microbiologia , Portador Sadio/virologia , Doenças Transmissíveis/epidemiologia , Doenças Transmissíveis/microbiologia , Doenças Transmissíveis/virologia , Humanos , Monitorização Fisiológica , Estudos SoroepidemiológicosAssuntos
Infecção Hospitalar/epidemiologia , Infecções por Klebsiella/epidemiologia , Klebsiella pneumoniae/isolamento & purificação , Adulto , Infecção Hospitalar/microbiologia , Resistência Microbiana a Medicamentos , Fezes/microbiologia , Humanos , Lactente , Recém-Nascido , Infecções por Klebsiella/microbiologia , Klebsiella pneumoniae/classificação , Klebsiella pneumoniae/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Enfermeiras e Enfermeiros , Sorotipagem , Sibéria/epidemiologia , Especificidade da Espécie , População Urbana/estatística & dados numéricosAssuntos
Testes de Fixação do Látex/métodos , Infecções Estafilocócicas/diagnóstico , Anticorpos Antibacterianos/sangue , Estudos de Avaliação como Assunto , Humanos , Técnicas Imunoenzimáticas , Fragmentos Fab das Imunoglobulinas/sangue , Látex , Tamanho da Partícula , Staphylococcus aureus/imunologiaRESUMO
The efficacies of detection of S. aureus by the bacteriologic method and enzyme immunoassay test system based on F(ab)2 fragments of purified antistaphylococcal antibodies were compared. Washings off the nasal mucosa and salivary samples from 20 normal subjects (medical staff) were examined. The number of findings of S. aureus by the detection of its a-hemotoxin in primary samples was much higher and the time of analysis two times shorter with enzyme immunoassay.
Assuntos
Proteínas Hemolisinas/análise , Técnicas Imunoenzimáticas , Mucosa Nasal/imunologia , Saliva/imunologia , Staphylococcus aureus/imunologia , Anticorpos Antibacterianos/imunologia , Técnicas Bacteriológicas , Humanos , Staphylococcus aureus/isolamento & purificação , Fatores de TempoRESUMO
As revealed in the realization of the epidemiological surveillance of hospital infections caused by methicillin-resistant S. aureus (MRSA) in different types of hospital, MRSA strains causing purulent inflammatory diseases belong to different clones. The complex marking of MRSA made it possible to determine the presence of the same clone in different hospitals and to detect the outbreaks of hospital infections caused by different clones of MRSA in one hospital. It was found necessary to supplement the commercial international phage-typing set with pages permitting the detection of the specific system of restriction-modification in MRSA.
Assuntos
Infecção Hospitalar/epidemiologia , Resistência a Meticilina , Vigilância da População , Infecções Estafilocócicas/epidemiologia , Staphylococcus aureus/isolamento & purificação , Antibacterianos/farmacologia , Tipagem de Bacteriófagos , Infecção Hospitalar/microbiologia , Humanos , Testes de Sensibilidade Microbiana , Federação Russa/epidemiologia , Estações do Ano , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/classificação , Staphylococcus aureus/efeitos dos fármacosRESUMO
In the study of vaginal microflora in parturient women, carriers of group B streptococci (GBS), and those free of carriership a wide range of microorganisms, represented by aerobic and anaerobic species with the prevalence of lactobacteria in both groups of women, was isolated. The composition of vaginal microflora in parturient women did not depend on the presence of GBS in this biotope or on the level of colonization by lactobacteria. The colonization resistance (CR) of the vaginal mucosa had essential influence of the level of carriership with respect to GBS and a number of representatives of the symbiotic microflora of this locus. In the majority of cases GBS were isolated from parturient women with low CR.
Assuntos
Bactérias/isolamento & purificação , Portador Sadio/microbiologia , Infecção Hospitalar/microbiologia , Trabalho de Parto , Infecções Estreptocócicas/microbiologia , Streptococcus agalactiae/isolamento & purificação , Vagina/microbiologia , Contagem de Colônia Microbiana , Feminino , Humanos , GravidezRESUMO
The microbiological surveillance of pneumococci according to the Protocols for the International Surveillance of Pneumococci Resistant to Penicillin and Other Antimicrobial Agents developed by Prof. M. Jacobs (USA) and Prof. P. Appelbaum (USA) made it possible for the first time in Russia to detect the strains with moderate resistance to penicillin. It is necessary to revise the current Russian recommendations for the laboratory diagnosis of pneumococcal infection and the determination of antibiotic susceptibility so as to participate in the united programme of the International Surveillance of Penicillin Resistance in Streptococcus pneumoniae.
Assuntos
Penicilina G/farmacologia , Resistência às Penicilinas , Vigilância da População , Streptococcus pneumoniae/efeitos dos fármacos , Pré-Escolar , Humanos , Lactente , Recém-Nascido , Testes de Sensibilidade Microbiana , Federação RussaRESUMO
In the approbation of the enzyme-linked immunosorbent assay (ELISA) system on the basis of F(ab)2 fragments of antistaphylococcal antibodies on 307 cultures of the representatives of the genera Staphylococcus, Pseudomonas and Proteus high sensitivity, specificity and effectiveness of ELISA for the quantitative and qualitative evaluation of the alpha-hemolytic activity of S.aureus were established. The ELISA system has made it possible to additionally detect alpha-hemolysin in 62% of S.aureus strains classified with as nontoxigenic strains using hemolysis test in Petri dishes. The sensitivity limit of this method is 0.0005 binding units or 1.0 ng in terms of protein content. The use of the ELISA system may be recommended for the study of the toxigenic properties of staphylococci.
Assuntos
Anticorpos Antibacterianos/imunologia , Toxinas Bacterianas/análise , Proteínas Hemolisinas/análise , Técnicas Imunoenzimáticas , Fragmentos Fab das Imunoglobulinas/imunologia , Staphylococcus aureus/imunologia , Análise de Variância , Especificidade de Anticorpos , Estudos de Avaliação como Assunto , Humanos , Técnicas Imunoenzimáticas/estatística & dados numéricos , Proteus/imunologia , Pseudomonas/imunologia , Sensibilidade e Especificidade , Espectrofotometria , Fatores de TempoRESUMO
The epidemiological analysis of hospital infection morbidity in Russia at the period of 1990-1991 made it possible to establish the occurrence of hospital infections among hospitalized patients throughout the country. The data on the structure of registered hospital infections were obtained. These data indicate that the prevalent part of hospital infections were neonatal purulent septic infections and postoperative infections; among the former, ophthalmic infections and infections of the skin and subcutaneous fat prevailed. The outbreaks of total morbidity in hospital infections did not exceed 1%, while the total morbidity in enteric infections was 10% in the whole of Russia and 30% in the territories where outbreaks of enteric infections were registered.
Assuntos
Infecção Hospitalar/epidemiologia , Adolescente , Adulto , Distribuição por Idade , Criança , Feminino , Humanos , Incidência , Recém-Nascido , Masculino , Federação Russa/epidemiologia , Infecção da Ferida Cirúrgica/epidemiologiaRESUMO
Microbiological monitoring of intrahospital infections in obstetric and surgical institutions revealed the role of Klebsiella pneumoniae in the etiology of purulent septic diseases in various groups of the patients. The level and spectra of resistance in the pathogens were fixed with respect to 17 drugs. It was shown that the clinical polyresistant strains of K. pneumoniae including those resistant to at least 5 antibiotics were more frequent in the patients of the surgical departments than in the newborns and puerpera. The isolates of K. pneumoniae responsible for the intrahospital complications in the surgical patients as compared to those in the newborns and puerpera were mainly resistant to ampicillin, carbenicillin, cephalothin, gentamicin and tobramycin.
Assuntos
Infecção Hospitalar/microbiologia , Klebsiella pneumoniae/fisiologia , Resistência Microbiana a Medicamentos , Humanos , Klebsiella pneumoniae/efeitos dos fármacos , Klebsiella pneumoniae/isolamento & purificação , Complicações Pós-Operatórias/microbiologiaAssuntos
Toxinas Bacterianas/análise , Staphylococcus aureus/imunologia , Anticorpos Antibacterianos/sangue , Toxinas Bacterianas/isolamento & purificação , Portador Sadio/microbiologia , Infecção Hospitalar/microbiologia , Humanos , Técnicas Imunoenzimáticas , Fragmentos Fab das Imunoglobulinas/sangue , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/isolamento & purificação , Fatores de TempoRESUMO
The production of toxins of the toxic shock syndrome and enterotoxins by S. aureus of clinical origin was studied by means of commercial standard antisera and standard toxins. The study revealed that among 511 strains isolated in Russia toxigenic ones constituted 41%, and among 592 strains isolated in Czechia toxigenic ones constituted 63%. The capacity for producing toxins may be used as an epidemiological marker.
Assuntos
Toxinas Bacterianas/biossíntese , Enterotoxinas/biossíntese , Choque Séptico/microbiologia , Staphylococcus aureus , Toxinas Bacterianas/análise , Biomarcadores/análise , Portador Sadio/epidemiologia , Portador Sadio/microbiologia , Tchecoslováquia/epidemiologia , Enterotoxinas/análise , Feminino , Humanos , Gravidez , Federação Russa/epidemiologia , Choque Séptico/epidemiologia , Staphylococcus aureus/isolamento & purificaçãoRESUMO
In order to study a possible etiological relationship between Clostridium and diarrhea in children of the first half year of life and to characterize the colonization of the intestine with these bacteria, bacteriological investigations of feces were carried out in neonates and babies aged 1, 4 and 14 days and 1, 3 and 6 months. The development of the children and their health status were monitored under home conditions. It has been established that the colonization of the neonates' intestine with Clostridium including C. Difficile occurs within the early times (since the 4th day of life). Later the colonization with C. difficile becomes wavy in nature. Among 7 types of Clostridium isolated from the intestine of the children, C. difficile occurred most frequently (29.1%). The overwhelming majority of the strains of these bacteria produced toxin whose activity did no exceed 10(-1)-10(-2). The cytopathic effect was mostly demonstrable in 72 hours. No convincing evidence was obtained about the etiological importance of C. difficile in the development of diarrhea in the children placed under observation. It is likely that the latter one was due to the disturbance of intestinal biocenosis, that manifested by profound quantitative disorders (proliferation of the opportunistic aerobic flora, a dramatic reduction of the content of bifido- and lactic acid bacteria up to their complete absence). At the same time a great number of children carrying C. difficile attests to a potential development of specific diarrheas (under hospital conditions and during massive antibacterial therapy).