Microbial acoustic sensor test-system based on a piezoelectric resonator with a lateral electric field for kanamycin detection in liquid.

A microbial test-system for real-time determination of low/residual concentrations of kanamycin in a liquid without the need for special labels is presented. The main element of the system was a piezoelectric resonator excited by a lateral electric field based on an X-cut lithium niobate plate 0.5 mm thick with two rectangular electrodes on one side. On the other side of the resonator, there was a 1.5 ml liquid container. As a sensory element we used Escherichia coli B-878 microbial cells, which are sensitive to kanamycin. For measurement 1 ml of this cells suspension was placed in a liquid container and then the test liquid in the amount of 2 µl containing kanamycin was added. The change in the real part of the electrical impedance of the resonator before and after the test liquid addition was used as an analytical signal which indicated the presence of kanamycin. The lower limit of determination of kanamycin turned out to be 1.0 µg/ml with an analysis time of 10 min. The test-system allows to detect kanamycin in the presence of such antibiotic as ampicillin and polymixin.

The sensor for measuring the micro-displacements based on the piezoelectric resonator with lateral electric field.

Theoretical and experimental studies of the influence of a thin metal film on the characteristics of a piezoelectric resonator with a lateral electric field showed the possibility of creating a micro-displacement meter in the range of 10-300 µm. For the experiments, two resonators based on the plates of PZT piezoceramics with a thickness of 3.56 and 4.46 mm with resonant frequencies of ~96 and ~260 kHz for both resonators were used. It has been experimentally established that in both cases, with an increase in the width of the gap between the free side of the piezoelectric resonator and thin aluminum film, the frequency of the parallel resonance and maximum value of the real part of the electrical impedance increase and reach saturation. Besides, it has been shown that the relative change of these values with a change in the width of the gap in the range 10-300 µm increases with decreasing the thickness of the resonator. In this case, the frequency of the series resonance practically does not change. It has been also established that the sensitivity of the resonator to the presence of a conducting film at the resonant frequency of ~96 kHz is significantly higher in comparison with the resonant frequency of ~260 kHz. The experimental results are in the qualitative agreement with the theoretical data.

The effect of the conductivity of a film located near a piezoelectric resonator with a lateral electric field based on the PZT ceramics on its characteristics.

In this paper, we investigated theoretically and experimentally the effect of the conductivity of the film located in the immediate vicinity of the free side of a piezoelectric resonator based on the PZT ceramics on its characteristics. It is shown that with increasing the conductivity of the film at first the parallel resonant frequency does not change, then it decreases in a certain interval and after that remains practically constant. With increasing the width of the gap between the film and the resonator, the resonant frequency increases and reaches the saturation. At that the degree of the total frequency change decreases with decreasing the conductivity of the film. We also investigated the dependence of the maximum value of the real part of the electrical impedance on the film conductivity at the different values of the width of the gap between the film and the resonator. The dependences of the maximum value of the real part of the electrical impedance on the width of the gap between the film and the resonator are also obtained for different values of the film conductivity. A comparison of the theoretical and experimental data showed their good qualitative agreement.

Analysis of the microbial cell-Ab binding in buffer solution by the piezoelectric resonator.

The possibility of the registration of the interaction of the cells Azospirillum lipoferum Sp59b with the specific antibodies directly in the conducting suspensions by using an acoustic sensor was shown. The main element of the sensor is a piezoelectric resonator with a lateral electric field. The analysis is based on a comparison of the resonator's electrical impedance before and after the specific biological interaction between the cells and antibodies. By using this sensor one can detect and identify the bacterial cells directly in the buffer solution with the conductivity between 2.4 and 20 µS/cm. The minimum detectable concentration of the bacterial cells turned out to be ∼103 cells/ml and for a short time (less than 10 min). Also the possibility of the detection of the cells in the presence of the extraneous microflora was shown. The results provide the opportunities for the development of a new class of the methods for the analysis of the microbial cells in real-time directly in the buffer solution.