The effect of interleukin-20 on periodontal tissue destruction in individuals with periodontitis.

BACKGROUND AND OBJECTIVE: Periodontitis is an inflammatory disease that destroys periodontal tissues. Interleukin-20 (IL-20), on the other hand, is known as a potent angiogenic, chemotactic, and pro-inflammatory cytokine associated with various chronic inflammatory disorders. IL-20 has a significant role in the regulation of osteoclastogenesis and osteoblastogenesis. This study aimed to evaluate the effect of IL-20 on periodontal destruction. METHODS: In this study, a total of 60 participants were included, 30 of whom were systemically and periodontally healthy (control group), and 30 were systemically healthy but had periodontitis (periodontitis group). Gingival crevicular fluid (GCF) and serum samples were collected from the participants for biochemical analysis. Enzyme-linked immunosorbent assay was used to determine the levels of IL-20, tumor necrosis factor (TNF)-α, IL1ß/IL-10, RANKL/osteoprotegerin (OPG), and matrix metalloproteinase-8 (MMP8). For statistical analysis, the independent t-test, Pearson correlation coefficients, and the Chi-square test were used. RESULTS: GCF IL-20, RANKL, RANKL/OPG, serum IL-20, RANKL, RANKL/OPG, MMP-8, TNF-α, IL-1B, and IL-1ß/IL-10 values were found to be statistically significantly higher in the periodontitis group than in the control group. GCF OPG and serum IL-10 values were found to be statistically significantly higher in the control group than in the periodontitis group. No statistically significant difference was observed between the groups in serum OPG values. A statistically significantly positive correlation was observed between serum IL-20 value and serum RANKL, RANKL/OPG, MMP-8, TNF-α, IL-1ß values, and periodontal clinical parameters. The ROC curves showed: AUC = 0.788 for GCF IL-20, and AUC = 1.000 for serum IL-20. CONCLUSION: According to the results of the study, IL-20 was found to be associated with periodontitis. The role of IL-20 in periodontal pathogenesis is related to osteoclastogenesis and collagen degradation. It is conceivable that IL-20 may increase bone destruction by both affecting the RANKL/OPG ratio and proinflammatory cytokines.

Evaluation of Breeding Values and Variance Components of Birth and Weaning Weights in the Holstein Cows Herd Based on Genetic Information.

Birth weights, weaning, and daily weight gain of calves before weaning vary according to farm animals' environment, production, and breeding systems. It is expected that these traits will be affected by direct genetic influences and the genetic effects of the mother. Therefore, this study was designed and conducted on 50 Holstein cows herds to estimate breeding value (after estimating the mean of allele effect and the mean of allele replacement effect of the DRB3 gene), as well as the variance components of the birth and weaning weight traits, depending on the variance resulting from the inherited polymorphism of the DBR3 gene. The results of this study showed a preference for the wild allele A based on its mutant counterpart B was the mean allele-effect value and the mean allele substitution value, corresponding to the preference for the A allele in both traits, the individuals with the AA genotype outperformed the homozygotes and mutants in the educational value (a value that is estimated based on what the individuals carry from the genotypes). The genetic variance of weaning weight was higher than the genetic variance of birth weight, and this may be due to the preference of allele A and the AA structure over the other two components in positively affecting birth and weaning weights, indicating the importance of this genetic structure of the DBR3 gene in selection within the genetic improvement programs for the color characteristic of Holstein cows.