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1.
Int J Mol Sci ; 24(14)2023 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-37511501

RESUMO

The Wnt/ß-catenin signaling pathway plays important roles in the multi-phases of wound healing: homeostasis, inflammation, proliferative, and remodeling phases. However, there are no clinically available therapeutic agents targeting the Wnt/ß-catenin pathway. In this study, we tested the effect of 5, 6-dichloroindirubin-3'-methoxime (KY19382), a small molecule that activates the Wnt/ß-catenin pathway via interference with the function of the negative feedback regulator CXXC5, on cutaneous wound healing. KY19382 significantly enhanced cell migration of human keratinocytes and dermal fibroblasts with increased levels of ß-catenin, phalloidin, Keratin 14, proliferating cell nuclear antigen (PCNA), Collagen I, and alpha-smooth muscle actin (α-SMA) by activating the Wnt/ß-catenin signaling pathway without causing significant cytotoxicity. In addition, levels of Collagen I, Keratin 14, PCNA, and stem cell markers were significantly increased by KY19382 in a cutaneous murine wound healing model. Moreover, KY19382 treatment accelerated re-epithelialization and neo-epidermis formation with collagen deposition and stem cell activation at an early stage of cutaneous wound healing. Overall, KY19382 accelerates wound healing via activating the Wnt/ß-catenin pathway, and may have the potential to be used for the development of a new wound healing agent.


Assuntos
Via de Sinalização Wnt , beta Catenina , Camundongos , Humanos , Animais , Via de Sinalização Wnt/fisiologia , beta Catenina/metabolismo , Queratina-14/metabolismo , Antígeno Nuclear de Célula em Proliferação/metabolismo , Proteínas Wnt/metabolismo , Cicatrização , Colágeno/farmacologia , Proteínas de Ligação a DNA/metabolismo , Fatores de Transcrição
2.
Exp Mol Med ; 55(8): 1770-1782, 2023 08.
Artigo em Inglês | MEDLINE | ID: mdl-37524876

RESUMO

Diabetic wound healing, including diabetic foot ulcer (DFU), is a serious complication of diabetes. Considering the complexity of DFU development, the identification of a factor that mediates multiple pathogeneses is important for treatment. In this study, we found that CXXC-type zinc finger protein 5 (CXXC5), a negative regulator of the Wnt/ß-catenin pathway, was overexpressed with suppression of the Wnt/ß-catenin pathway and its target genes involved in wound healing and angiogenesis in the wound tissues of DFU patients and diabetes-induced model mice. KY19334, a small molecule that activates the Wnt/ß-catenin pathway by inhibiting the CXXC5-Dvl interaction, accelerated wound healing in diabetic mice. The enhancement of diabetic wound healing could be achieved by restoring the suppressed Wnt/ß-catenin signaling and subsequently inducing its target genes. Moreover, KY19334 induced angiogenesis in hindlimb ischemia model mice. Overall, these findings revealed that restorative activation of Wnt/ß-catenin signaling by inhibiting the function of cytosolic CXXC5 could be a therapeutic approach for treating DFUs.


Assuntos
Diabetes Mellitus Experimental , Pé Diabético , Cicatrização , Animais , Camundongos , beta Catenina/metabolismo , Diabetes Mellitus Experimental/complicações , Pé Diabético/metabolismo , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Fatores de Transcrição/genética , Via de Sinalização Wnt/fisiologia , Cicatrização/fisiologia , Humanos
3.
Adv Healthc Mater ; 12(18): e2203094, 2023 07.
Artigo em Inglês | MEDLINE | ID: mdl-36854308

RESUMO

Regenerative wound healing involves the scarless wound healing as observed in fetal skin. Multiple features of regenerative wound healing have been well studied; however, the practical application of pro-regenerative materials to recapitulate the regenerative wound healing in adult skins has not yet been achieved. In this study, the authors identified that their novel pro-regenerative material, pyrogallol-functionalized hyaluronic acid (HA-PG) patches in combination with protein transduction domain-fused Dishevelled (Dvl)-binding motif (PTD-DBM), a peptide inhibiting the CXXC-type zinc finger protein 5 (CXXC5)-Dvl interaction, promoted regenerative wound healing in mice. The HA-PG patches loaded with this competitor peptide and valproic acid (VPA), a glycogen synthase kinase 3ß (GSK3ß) inhibitor, significantly inhibited scar formation during wound healing. The HA-PG patches with PTD-DBM and/or VPA inhibit the expression of differentiated cell markers such as α-smooth muscle actin (α-SMA) while inducing the expression of stem cell markers such as CD105 and Nestin. Moreover, Collagen III, an important factor for regenerative healing, is critically induced by the HA-PG patches with PTD-DBM and/or VPA, as also seen in VPA-treated Cxxc5-/- mouse fibroblasts. Overall, these findings suggest that the novel regeneration-promoting material can be utilized as a potential therapeutic agent to promote both wound healing and scar attenuation.


Assuntos
Cicatriz , Hidrogéis , Animais , Camundongos , Cicatriz/tratamento farmacológico , Hidrogéis/farmacologia , Cicatrização/fisiologia , Peptídeos , Quimioterapia Combinada , Proteínas de Ligação a DNA , Fatores de Transcrição
4.
Sci Rep ; 12(1): 20669, 2022 11 30.
Artigo em Inglês | MEDLINE | ID: mdl-36450849

RESUMO

Obesity has become a major risk factor for developing metabolic diseases, including insulin resistance, type 2 diabetes, and hypertension. Growing pieces of evidence indicate that the Wnt/ß-catenin signaling pathway plays an important role in adipogenesis and obesity. Activation of the Wnt/ß-catenin signaling pathway inhibits adipogenesis by suppressing the differentiation of committed preadipocytes into mature adipocytes. CXXC5 is highly induced with suppression of Wnt/ß-catenin signaling in early adipogenic differentiation. In addition, silencing CXXC5 in vitro increased ß-catenin and decremented the major adipogenic differentiation markers. KY19334, a small molecule that activates the Wnt/ß-catenin pathway via inhibition of CXXC5- Dishevelled (Dvl) protein-protein interaction (PPI), suppressed adipogenic differentiation. Administration of KY19334 ameliorated obesity by 26 ± 1.3% and insulin resistance by 23.45 ± 7.09% and reduced adipocyte hypertrophy by 80.87 ± 5.30% in high-fat diet (HFD)-fed mice. In addition, KY19334 accelerated the browning of adipose tissue and promoted hepatic glucose homeostasis in HFD-fed mice. In conclusion, activation of the Wnt/ß-catenin signaling by inhibiting the interaction of CXXC5 and Dvl by small molecule-mediated interference is a potential therapeutic approach for treating obesity and insulin resistance.


Assuntos
Diabetes Mellitus Tipo 2 , Resistência à Insulina , Camundongos , Animais , Adipogenia , beta Catenina , Obesidade/tratamento farmacológico , Proteínas de Ligação a DNA/genética , Fatores de Transcrição/genética
5.
Molecules ; 27(20)2022 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-36296727

RESUMO

The activation of the Wnt/ß-catenin signaling pathway plays a key role in the wound-healing process through tissue regeneration. The extract of Euodia daniellii Hemsl. (E. daniellii), a member of the Rutaceae family, activates the Wnt/ß-catenin signaling pathway. However, the function of E. daniellii in wound healing has not yet been elucidated. We performed a migration assay to determine the wound-healing effect of E. daniellii extract in vitro using human keratinocytes and dermal fibroblast. In addition, a mouse acute wound model was used to investigate the cutaneous wound-healing effect of E. daniellii extract in vivo and confirm the potential mechanism. E. daniellii extract enhanced the migration of human keratinocytes and dermal fibroblasts via the activation of the Wnt/ß-catenin pathway. Moreover, the E. daniellii extract increased the levels of keratin 14, PCNA, collagen I, and α-SMA, with nuclei accumulation of ß-catenin in vitro. E. daniellii extract also efficiently accelerated re-epithelialization and stimulated wound healing in vivo. Furthermore, we confirmed that hesperidin, one of the components of E. daniellii, efficiently accelerated the migration of human keratinocytes and dermal fibroblasts, as well as wound healing in vivo via the activation of the Wnt/ß-catenin pathway. Overall, E. daniellii extract and its active component, hesperidin, have potential to be used as therapeutic agents for wound healing.


Assuntos
Evodia , Hesperidina , Camundongos , Animais , Humanos , Via de Sinalização Wnt , beta Catenina/metabolismo , Queratina-14/metabolismo , Hesperidina/farmacologia , Evodia/metabolismo , Antígeno Nuclear de Célula em Proliferação/metabolismo , Cicatrização , Colágeno/metabolismo , Fibroblastos/metabolismo
6.
Exp Mol Med ; 54(9): 1511-1523, 2022 09.
Artigo em Inglês | MEDLINE | ID: mdl-36114279

RESUMO

Non-alcoholic steatohepatitis (NASH) is a chronic liver disease that results from multiple metabolic disorders. Considering the complexity of the pathogenesis, the identification of a factor mediating the multiple pathogenic phenotypes of NASH will be important for treatment. In this study, we found that CXXC5, a negative feedback regulator of the Wnt/ß-catenin pathway, was overexpressed with suppression of Wnt/ß-catenin signaling and its target genes involved in hepatic metabolism in obese-NASH patients. Cxxc5-/- mice were found to be resistant to NASH pathogenesis with metabolic improvements. KY19334, a small molecule that activates the Wnt/ß-catenin pathway via interference of the CXXC5-Dvl interaction, reversed the overall pathogenic features of NASH as Cxxc5-/- mice. The improvement in NASH by KY19334 is attributed to its regenerative effects through restorative activation of the suppressed Wnt/ß-catenin signaling. Overall, the pronounced metabolic improvements with the stimulation of liver regeneration by interfering with the CXXC5-Dvl interaction provide a therapeutic approach for NASH.


Assuntos
Hepatopatia Gordurosa não Alcoólica , beta Catenina , Animais , Proteínas de Ligação a DNA/metabolismo , Fígado/metabolismo , Regeneração Hepática , Camundongos , Hepatopatia Gordurosa não Alcoólica/tratamento farmacológico , Hepatopatia Gordurosa não Alcoólica/etiologia , Fatores de Transcrição , Via de Sinalização Wnt/fisiologia , beta Catenina/genética , beta Catenina/metabolismo
7.
Clin Transl Med ; 12(4): e742, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-35384342

RESUMO

BACKGROUND: Metabolic diseases, including type 2 diabetes, have long been considered incurable, chronic conditions resulting from a variety of pathological conditions in obese patients. Growing evidence suggests the Wnt/ß-catenin pathway is a major pathway in adipose tissue remodelling, pancreatic ß-cell regeneration and energy expenditure through regulation of key metabolic target genes in various tissues. CXXC5-type zinc finger protein 5 (CXXC5) is identified negative feedback regulator of the Wnt/ß-catenin pathway that functions via Dishevelled (Dvl) binding. METHODS: Expression level of CXXC5 was characterised in clinical samples and diabetes-induced mice model. Diabetes-induced mice model was established by using high-fat diet (HFD). HFD-fed mice treated with KY19334, a small molecule inhibiting CXXC5-Dvl protein-protein interaction (PPI), was used to assess the role of CXXC5 in metabolic diseases. RESULTS: Here, we show that CXXC5 is overexpressed with suppression of Wnt/ß-catenin signalling in visceral adipose tissues of patients with obesity-related diabetes. Meanwhile, Cxxc5-/- mice fed an HFD exhibited resistance to metabolic dysregulation. KY19334 restores the lowered Wnt/ß-catenin signalling and reverses metabolic abnormalities as observed in HFD-fed Cxxc5-/- mice. Administration of KY19334 on HFD-fed mice had a long-lasting glucose-controlling effect through remodelling of adipocytes and regeneration of pancreatic ß-cells. CONCLUSION: Overall, the inhibition of CXXC5 function by small molecule-mediated interference of Dvl binding is a potential therapeutic strategy for the treatment of obesity-related diabetes.


Assuntos
Proteínas de Ligação a DNA , Diabetes Mellitus Tipo 2 , Fatores de Transcrição , Adipócitos/metabolismo , Tecido Adiposo/metabolismo , Animais , Proteínas de Ligação a DNA/antagonistas & inibidores , Proteínas de Ligação a DNA/metabolismo , Diabetes Mellitus Tipo 2/complicações , Diabetes Mellitus Tipo 2/tratamento farmacológico , Diabetes Mellitus Tipo 2/genética , Humanos , Camundongos , Camundongos Knockout , Obesidade/complicações , Obesidade/tratamento farmacológico , Obesidade/genética , Fatores de Transcrição/antagonistas & inibidores , Fatores de Transcrição/metabolismo , Via de Sinalização Wnt
8.
J Cell Physiol ; 236(7): 5193-5211, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-33368247

RESUMO

Phospholipase D (PLD) isoforms PLD1 and PLD2 serve as the primary nodes where diverse signaling pathways converge. However, their isoform-specific functions remain unclear. We showed that PLD1 and PLD2 selectively couple to toll-like receptor 4 (TLR4) and interleukin 4 receptor (IL-4R) and differentially regulate macrophage polarization of M1 and M2 via the LPS-MyD88 axis and the IL-4-JAK3 signaling, respectively. Lipopolysaccharide (LPS) enhanced TLR4 or MyD88 interaction with PLD1; IL-4 induced IL-4R or JAK3 association with PLD2, indicating isozyme-specific signaling events. PLD1 and PLD2 are indispensable for M1 polarization and M2 polarization, respectively. Genetic and pharmacological targeting of PLD1 conferred protection against LPS-induced sepsis, cardiotoxin-induced muscle injury, and skin injury by promoting the shift toward M2; PLD2 ablation intensified disease severity by promoting the shift toward M1. Enhanced Foxp3+ regulatory T cell recruitment also influenced the anti-inflammatory phenotype of Pld1LyzCre macrophages. We reveal a previously uncharacterized role of PLD isoforms in macrophage polarization, signifying potential pharmacological interventions for macrophage modulation.


Assuntos
Macrófagos/fisiologia , Fosfolipase D/metabolismo , Cicatrização/fisiologia , Ferimentos e Lesões/prevenção & controle , Animais , Polaridade Celular/fisiologia , Inflamação/patologia , Inflamação/prevenção & controle , Janus Quinase 3/metabolismo , Lipopolissacarídeos , Macrófagos/imunologia , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Músculos/lesões , Fator 88 de Diferenciação Mieloide/metabolismo , Fosfolipase D/genética , Receptores de Interleucina-4/metabolismo , Sepse/imunologia , Linfócitos T Reguladores/imunologia , Receptor 4 Toll-Like/metabolismo , Ferimentos e Lesões/patologia
9.
Pharmaceutics ; 12(2)2020 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-32093032

RESUMO

Indirubin is an active component of Dang Gui Long Hui Wan, which has been used in traditional Chinese medicine to treat inflammatory diseases as well as for the prevention and treatment of human cancer, such as chronic myeloid leukemia. The therapeutic effects of indirubin analogs have been underestimated due to its poor water solubility and low bioavailability. To improve the solubility and bioavailability of indirubin analogs, we prepared a mixed micellar formulation with Kolliphor® EL and Tween 80 as surfactants, and PEG 400 as a co-surfactant, followed by complexation with (2-hydroxyproply)-ß-cyclodextrin at appropriate ratios. Overall, improving the solubility and skin penetration of indirubin analogs can increase clinical efficacy and provide maximum flux through the skin.

10.
Sci Rep ; 9(1): 19676, 2019 12 23.
Artigo em Inglês | MEDLINE | ID: mdl-31873167

RESUMO

Ras/MAPK (mitogen active protein kinase) signaling plays contradictory roles in adipocyte differentiation and is tightly regulated during adipogenesis. However, mechanisms regulating adipocyte differentiation involving Ras protein stability regulation are unknown. Here, we show that WD40 repeat protein 76 (WDR76), a novel Ras regulating E3 linker protein, controls 3T3-L1 adipocyte differentiation through HRas stability regulation. The roles of WDR76 in obesity and metabolic regulation were characterized using a high-fat diet (HFD)-induced obesity model using Wdr76-/- mice and liver-specific Wdr76 transgenic mice (Wdr76Li-TG). Wdr76-/- mice are resistant to HFD-induced obesity, insulin resistance and hyperlipidemia with an increment of HRas levels. In contrast, Wdr76Li-TG mice showed increased HFD-induced obesity, insulin resistance with reduced HRas levels. Our findings suggest that WDR76 controls HFD-induced obesity and hepatic steatosis via HRas destabilization. These data provide insights into the links between WDR76, HRas, and obesity.


Assuntos
Proteínas de Ciclo Celular/metabolismo , Proteínas de Ligação a DNA/metabolismo , Fígado Gorduroso/metabolismo , Obesidade/metabolismo , Proteínas Proto-Oncogênicas p21(ras)/metabolismo , Células 3T3-L1 , Adipócitos/metabolismo , Adipogenia/fisiologia , Animais , Proteínas de Ciclo Celular/deficiência , Proteínas de Ciclo Celular/genética , Diferenciação Celular/fisiologia , Proteínas de Ligação a DNA/deficiência , Proteínas de Ligação a DNA/genética , Dieta Hiperlipídica/efeitos adversos , Modelos Animais de Doenças , Fígado Gorduroso/etiologia , Fígado Gorduroso/patologia , Hiperlipidemias/etiologia , Hiperlipidemias/metabolismo , Resistência à Insulina/genética , Resistência à Insulina/fisiologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Camundongos Transgênicos , Obesidade/etiologia , Estabilidade Proteica , Proteínas Proto-Oncogênicas p21(ras)/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo
11.
Life Sci Alliance ; 2(2)2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30971423

RESUMO

Longitudinal bone growth ceases with growth plate senescence during puberty. However, the molecular mechanisms of this phenomenon are largely unexplored. Here, we examined Wnt-responsive genes before and after growth plate senescence and found that CXXC finger protein 5 (CXXC5), a negative regulator of the Wnt/ß-catenin pathway, was gradually elevated with reduction of Wnt/ß-catenin signaling during senescent changes of rodent growth plate. Cxxc5 -/- mice demonstrated delayed growth plate senescence and tibial elongation. As CXXC5 functions by interacting with dishevelled (DVL), we sought to identify small molecules capable of disrupting this interaction. In vitro screening assay monitoring CXXC5-DVL interaction revealed that several indirubin analogs were effective antagonists of this interaction. A functionally improved indirubin derivative, KY19382, elongated tibial length through delayed senescence and further activation of the growth plate in adolescent mice. Collectively, our findings reveal an important role for CXXC5 as a suppressor of longitudinal bone growth involving growth plate activity.


Assuntos
Desenvolvimento Ósseo/fisiologia , Proteínas de Ligação a DNA/metabolismo , Lâmina de Crescimento/metabolismo , Fatores de Transcrição/metabolismo , Animais , Proteínas de Ligação a DNA/genética , Proteínas Desgrenhadas/metabolismo , Células HEK293 , Humanos , Indóis/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Transdução de Sinais/efeitos dos fármacos , Fatores de Transcrição/genética , Transfecção , Via de Sinalização Wnt , beta Catenina/metabolismo
12.
Biomaterials ; 167: 69-79, 2018 06.
Artigo em Inglês | MEDLINE | ID: mdl-29554482

RESUMO

The interest in alternative material systems and delivery methods for treatment of androgenetic alopecia has been increasing in the recent decades. Topical application of valproic acid (VPA), an FDA-approved anticonvulsant drug, has been shown to effectively stimulate hair follicle (HF) regrowth by upregulating Wnt/ß-catenin, a key pathway involved in initiation of HF development. Moreover, a majority of studies have suggested that cutaneous wound re-epithelialization is capable of inducing HF through Wnt/ß-catenin pathway. Here, we report fabrication and evaluation of a novel VPA-encapsulating dissolving microneedle (DMN-VPA) that creates minimally invasive dermal micro-wounds upon application, significantly improving the VPA delivery efficiency. DMN-VPA not only delivers encapsulated VPA with higher accuracy than topical application, it also stimulates wound re-epithelialization signals involved in HF regrowth. Through a series of in vivo studies, we show that micro-wounding-mediated implantation of DMN-VPA upregulates expression of Wnt/ß-catenin pathway, alkaline phosphatase, proliferating cell nuclear antigen, loricrin and HF stem cell markers, including keratin 15, and CD34 more effectively than topical application.


Assuntos
Sistemas de Liberação de Medicamentos/instrumentação , Cabelo/efeitos dos fármacos , Cabelo/crescimento & desenvolvimento , Ácido Valproico/administração & dosagem , Ácido Valproico/farmacologia , Administração Tópica , Animais , Anticonvulsivantes/administração & dosagem , Anticonvulsivantes/farmacologia , Linhagem Celular , Folículo Piloso/efeitos dos fármacos , Folículo Piloso/crescimento & desenvolvimento , Humanos , Masculino , Camundongos , Agulhas
13.
J Invest Dermatol ; 137(11): 2260-2269, 2017 11.
Artigo em Inglês | MEDLINE | ID: mdl-28595998

RESUMO

The Wnt/ß-catenin pathway has been implicated in hair follicle development and hair regeneration in adults. We discovered that CXXC-type zinc finger protein 5 (CXXC5) is a negative regulator of the Wnt/ß-catenin pathway involved in hair regrowth and wound-induced hair follicle neogenesis via an interaction with Dishevelled. CXXC5 was upregulated in miniaturized hair follicles and arrector pili muscles in human balding scalps. The inhibitory effects of CXXC5 on alkaline phosphatase activity and cell proliferation were demonstrated using human hair follicle dermal papilla cells. Moreover, CXXC5-/- mice displayed accelerated hair regrowth, and treatment with valproic acid, a glycogen synthase kinase 3ß inhibitor that activates the Wnt/ß-catenin pathway, further induced hair regrowth in the CXXC5-/- mice. Disrupting the CXXC5-Dishevelled interaction with a competitor peptide activated the Wnt/ß-catenin pathway and accelerated hair regrowth and wound-induced hair follicle neogenesis. Overall, these findings suggest that the CXXC5-Dishevelled interaction is a potential target for the treatment of hair loss.


Assuntos
Alopecia/genética , Regulação da Expressão Gênica , Folículo Piloso/patologia , Peptídeos e Proteínas de Sinalização Intracelular/genética , Ácido Valproico/farmacologia , Ferimentos e Lesões/complicações , Alopecia/etiologia , Alopecia/patologia , Animais , Células Cultivadas/efeitos dos fármacos , Proteínas de Ligação a DNA , Modelos Animais de Doenças , Proteínas Desgrenhadas , Cabelo/diagnóstico por imagem , Cabelo/efeitos dos fármacos , Folículo Piloso/metabolismo , Humanos , Camundongos , Peptídeos/farmacologia , Distribuição Aleatória , Regeneração/genética , Fatores de Transcrição , Regulação para Cima , Via de Sinalização Wnt/genética , Via de Sinalização Wnt/fisiologia , beta Catenina/metabolismo
14.
Biochem Biophys Res Commun ; 485(3): 584-590, 2017 Apr 08.
Artigo em Inglês | MEDLINE | ID: mdl-27932247

RESUMO

Dishevelled (Dvl) plays a crucial role in Wnt signaling by interacting with membrane-bound receptors and downstream molecules through its PDZ domain. CXXC5 is one of the key molecules that interacts with Dvl and negatively regulates the Wnt/ß-catenin pathway in osteoblast differentiation. Recently, the Dvl-CXXC5 interaction has been identified as an excellent target for osteoporosis treatment. Therefore, it is desirable to have detailed structural information for the Dvl-CXXC5 interaction. Although solution structures of the Dvl1 PDZ domain have been reported, a high-resolution crystal structure would provide detailed sidechain information that is essential for drug development. Here, we determined the first crystal structure of the Dvl-1 PDZ domain at a resolution of 1.76 Å, and compared it with its previously reported solution structure. The Dvl1 PDZ domain crystal belonged to the space group H32 with unit-cell parameters a = b = 72.837, c = 120.616, α = ß = 90.00, γ = 120.00. The crystal structure of Dvl1 PDZ shared its topology with the previously reported structure determined by nuclear magnetic resonance (NMR); however, the crystal structure was quite different from the solution structure in both the secondary structural region and the ligand-binding pocket. Molecular modeling based on NMR and X-ray crystallographic data yielded detailed information about the Dvl1/CXXC5 interaction, which will be useful for designing inhibitors.


Assuntos
Proteínas Desgrenhadas/química , Peptídeos e Proteínas de Sinalização Intracelular/química , Domínios PDZ , Estrutura Secundária de Proteína , Sequência de Aminoácidos , Animais , Sítios de Ligação/genética , Cristalização , Cristalografia por Raios X , Proteínas de Ligação a DNA , Proteínas Desgrenhadas/genética , Proteínas Desgrenhadas/metabolismo , Peptídeos e Proteínas de Sinalização Intracelular/genética , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Ligantes , Espectroscopia de Ressonância Magnética , Camundongos , Modelos Moleculares , Peptídeos/química , Peptídeos/metabolismo , Ligação Proteica , Homologia de Sequência de Aminoácidos , Soluções , Fatores de Transcrição , Via de Sinalização Wnt
15.
Phytother Res ; 30(5): 848-54, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-26929003

RESUMO

Polygonum aviculare L. is a member of the Polygonaceae family of plants, which has been known for its antioxidant and anti-obesity effects. However, the wound healing function of P. aviculare extract has not been assessed. In this study, we identified a novel property of P. aviculare extract as a Wnt/ß-catenin pathway activator based on a screen of 350 plant extracts using HEK293-TOP cells retaining the Wnt/ß-catenin signaling reporter gene. P. aviculare extract accelerated the migration of HaCaT keratinocytes without showing significant cytotoxicity. Moreover, P. aviculare extract efficiently re-epithelized wounds generated on mice. Additionally, ingredients of P. aviculare extract, such as quercitrin hydrate, caffeic acid, and rutin, also accelerated the motility of HaCaT keratinocytes with the activation of Wnt/ß-catenin signaling. Therefore, based on our findings, P. aviculare extract and its active ingredients could be potential therapeutic agents for wound healing. Copyright © 2016 John Wiley & Sons, Ltd.


Assuntos
Ácidos Cafeicos/química , Extratos Vegetais/química , Polygonum/química , Quercetina/análogos & derivados , Cicatrização/efeitos dos fármacos , beta Catenina/metabolismo , Animais , Humanos , Masculino , Camundongos , Extratos Vegetais/farmacologia , Quercetina/química , Transfecção , Via de Sinalização Wnt
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