RESUMO
Polyvalent Pseudomonas aeruginosa vaccine, prepared at the Institute of Hematology from 10 hospital strains isolated from burn wounds, was administered to 32 children with extensive and deep burns. The vaccine was well tolerated. The vaccine produced a high degree of the immunity against Pseudomonas aeruginosa infection. Agglutinin serum titre increased significantly. Vaccination either prevented or inhibited the infection of burn wounds with Pseudomonas aeruginosa in all immunized children. The symptoms of Pseudomonas aeruginosa infection usually disappeared following one or two vaccinations. Bacteriemia caused by P. aeruginosa was not observed in 31 out of 32 children. In the remaining child transient bacteriemia was noted. No septicemia caused by P. aeruginosa was seen. Due to the high efficiency of the polyvalent P. aeruginosa vaccine all burned children with burns exceeding 10% of the total body surface should by vaccinated to prevent the life-threatening infections with Pseudomonas aeruginosa.
Assuntos
Vacinas Bacterianas/administração & dosagem , Queimaduras/complicações , Infecções por Pseudomonas/prevenção & controle , Pseudomonas aeruginosa/imunologia , Infecção dos Ferimentos/prevenção & controle , Aglutininas/análise , Anticorpos Antibacterianos/análise , Vacinas Bacterianas/imunologia , Queimaduras/imunologia , Criança , Pré-Escolar , Humanos , Polônia , Infecções por Pseudomonas/imunologia , Infecção dos Ferimentos/imunologiaAssuntos
Queimaduras/terapia , Colágeno/administração & dosagem , Administração Tópica , Animais , Bandagens , Bovinos , PolôniaAssuntos
Bandagens , Queimaduras/microbiologia , Administração Tópica , Anti-Inflamatórios/administração & dosagem , Bactérias/efeitos dos fármacos , Combinação de Medicamentos/administração & dosagem , Gentamicinas/administração & dosagem , Humanos , Hidrocortisona , Neomicina/administração & dosagem , Oleandomicina/administração & dosagem , Pele/microbiologia , Soluções , Tetraciclina/administração & dosagemAssuntos
Antibacterianos/uso terapêutico , Queimaduras/microbiologia , Tobramicina/uso terapêutico , Bactérias/efeitos dos fármacos , Infecções Bacterianas/prevenção & controle , Resistência Microbiana a Medicamentos , Humanos , Técnicas In Vitro , Pele/microbiologia , Infecção dos Ferimentos/prevenção & controleRESUMO
The authors assessed the phagocytic ability of granulocytes, the ability of blastic transformation of lymphocytes after phytohaemagglutinin (PHA) stimulation in vitro culture, and the viability and number of these cells before freezing and after thawing. No significant differences were found in the quality of granulocytes stored in cryoprotective medium containing 13% of glycerol or 15% of PVP. On the other hand, much worse results were obtained in both cases in comparison with previous investigations in which DMSO was used as the protective agent. It was observed also that lympocytes stored in a cryoprotective medium containing 15% of PVP were not significantly depressed and retained their ability of blastic transformation in vitro under PHA stimulation. Similar results were obtained using in previous investigations a cryoprotective medium with DMSO.