RESUMO
The paper presents the results of biopsy and curettage specimens from 92 patients with infertility. Histological and immunohistological (steroid receptors, Ki67, P27, and cyclin E) methods and scanning electron microscopy have been used. The substantial immunohistochemical (IHC) changes found in the endometrial implantation window in infertility are associated with the spectrum of the detected pathology. No clear correlations between the expression of IHC markers and the concentrations of serum hormones, which are characteristic of the normal menstrual cycle, may suggest that there is an imbalance of steroid reception at the endometrial tissue level. The detection of proteins P27 and cyclin E in the cytoplasm of glandular and stromal cells reflects the deficient block of a cell cycle essential for an adequate implantation window. The detected features of the superficial endometrial ultrastructure may be an additional indicator of the adequacy of endometrial secretory maturation. The essential histological, immunohistochemical, and ultrastructural features of the endometrium in infertility give valuable information that complements the traditional clinical classifications. Evaluation of the endometrial morphofunctional state opens up fresh opportunities to ensure an individual approach when using the regimens of assisted reproductive technologies.
Assuntos
Ciclina E/biossíntese , Endométrio , Regulação da Expressão Gênica , Infertilidade Feminina , Antígeno Ki-67/biossíntese , Antígeno Nuclear de Célula em Proliferação/biossíntese , Receptores de Esteroides/biossíntese , Adulto , Biomarcadores/metabolismo , Endométrio/metabolismo , Endométrio/ultraestrutura , Feminino , Humanos , Infertilidade Feminina/metabolismo , Infertilidade Feminina/patologiaRESUMO
Endometrial scrapes and biopsy specimens from 47 females with primary and secondary infertility were studied by histological and immnohistological (estrogen and progesterone receptors, Ki67) methods. Endometrial abnormalities, such as luteal phase defect, hyperplasia, endometrial polyps, and chronic endometritis, were detected in all the patients. There were considerable changes in the expression of estrogen and progesterone receptors and impaired endometrial proliferative processes. There was no clear correlation of immunohistochemical parameters with blood hormone levels in both primary and secondary infertility. The findings may be of significance in choosing the schemes of assisted procreation technologies.
