[The alpha-defensins, peptides and proteins synthesized and liberated by neutrophils under atherosclerosis of different localization].

In recent years, the scientific publications about disorder the scientific publications appeared concerning derangement of content of alpha-defensins in neutrophils of patients with atherosclerosis. The interest increases both to different derangement of protein-synthesizing function of neutrophils and to biological effects caused by defensins and other peptides and proteins taking part in anti-microbial defense of human organism. With purpose to study content of alpha-defensins (1-3) and synthesis and liberation of other proteins and peptides by neutrophils the concentrations of alpha-defensins, lipoprotein (alpha), C-reactive protein, precursor of cerebral natriuretic peptide, coagulation factor VII and von Willebrand factor were determined in supernatants of leukocytal cultures in patients with exertional angina pectoris and atherosclerosis of lower extremities. The evaluation of loading test was implemented in vitro with alpha-tumor necrosis factor in vitro. Simultaneously, serum concentrations of cytokines and pro-atherogenic proteins were analyzed. It is established that in patients with ischemic heart disease derangement of protein-synthesizing function of neutrophils is observed. The derangement is manifested in the form of decrease of synthesis of alpha-defensins, increase of synthesis of lipoprotein (alpha), precursor of cerebral natriuretic peptide, C-reactive protein and von Willebrand factor. The pathogenic role of alpha-tumor necrosis factor is established. The increase of concentration of interleukin-8 in blood serum is revealed.

[Protein-synthesizing function of neutrophils and lipid-releasing ability of leukocytes in patients with atherosclerosis].

Concentrations of defensins-alpha, lipoprotein(α), C-reactive protein, proBNP, VII- coagulation factor and von Wilebrandt factor were measured in supernatants of 3-day leukocyte cultures enriched with neutrophils in order to estimate their synthesis in patients with stable angina and atherosclerosis of lower extremities. In addition, in vitro tumor necrosis factor-alpha test was performed. Simultaneously, concentrations of serum interleukine-6, -8, defensins-alpha, C-reactive protein, proBNP, VII-coagulation factor were determined. Neutrophil protein synthesis was impaired in the patients with stable angina. The same was true of defensins-alpha synthesis whereas production of lipoproteins, pro-BNP, C-reactive proteins, IL-8, and von Wilebrandt factor increased. Pathogenetic role of TNF-alpha was demonstrated.