Comparison of microbial diversity and metabolic activities in organic and conventional rice farms in Thailand.

Studies of the soil microbial community have revealed that several factors, both biotic and abiotic, can influence the community structure, diversity, and function. Human agricultural practice has been one of the key factors that affect microbial structure in soil. In this study, we examined the effect of two rice farming practices (i.e., conventional and organic) on soil microbial diversity and their metabolic activities. We proposed that the use of herbicides and chemical fertilizers in the conventional rice farming may decrease the soil microbial diversity and that it may lead to a decline in soil quality. However, our results show that there is no significant difference in the soil chemical properties, microbial diversity, and microbial metabolic activities between the two agricultural management systems. The wetland water cycling regime of rice agriculture in Thailand perhaps prevents the accumulation of chemicals in the soil, thus subdue the effect of these chemicals on soil microbes. Furthermore, our results showed that the key determinant of soil chemical properties as well as microbial community structure and diversity is the soil physical property, while the farming activity influences the microbial metabolic functions. These findings indicate the complex nature of the soil, microbes, and human farming interactions.IMPORTANCERice is a major export commodity in Thailand. As such, an understanding of the effect of conventional or organic farming approaches on soil microbial community could enable a suitable farming management. In this study, microbial communities were surveyed and compared between the two rice farming practices for their diversity and metabolic activities. Results showed no significant differences in microbial community structure and diversity between the two rice farming practices, but significant differences were observed due to the soil type, namely, clay, silty clay, silty clay loam, loam, and silty loam. Interestingly, significant differences in metabolic functions were also observed in different soil farming activities, such as land rest, period of growth, and post-burning, but not due to conventional or organic practices. These findings showed that the soil physical type and the farming activity impact the microbial community more than whether it is conventional or organically farmed.

Fire-Induced Changes in Soil Properties and Bacterial Communities in Rotational Shifting Cultivation Fields in Northern Thailand.

Fire is a common practice in rotational shifting cultivation (RSC), but little is known about the dynamics of bacterial populations and the impact of fire disturbance in northern Thailand. To fill the research gap, this study aims to investigate the dynamics of soil bacterial communities and examine how the soil's physicochemical properties influence the bacterial communities in RSC fields over a period of one year following a fire. Surface soil samples (0-2 cm depth) were collected from sites with 6 (RSC-6Y) and 12 (RSC-12Y) years of fallow in Chiang Mai Province, northern Thailand at six different time points: before burning, 5 min after burning (summer), 3 months after burning (rainy season), 6 months after burning (rainy season), 9 months after burning (winter), and 12 months after burning (summer). The results revealed a reduction in the soil bacterial communities' diversity and an increase in soil nutrient levels immediately after the fire. The fire significantly influenced the abundance of Firmicutes, Proteobacteria, Acidobacteria, and Planctomycetes, but not that of Actinobacteria. At the genus level, Bacillus, Conexibacter, and Chthoniobacter showed increased abundance following the fire. During the rainy season, a recovery in the abundance of the soil bacterial communities was observed, although soil nutrient availability declined. Soil physicochemical properties such as pH, organic matter, organic carbon, electrical conductivity, cation exchange capacity, nitrate-nitrogen, available phosphorus, exchangeable potassium, total nitrogen, bulk density, sand, and silt contents significantly influenced the composition of bacterial communities. Alpha diversity indices indicated a decrease in diversity immediately after burning, followed by an increase from the early rainy season until the summer season, indicating that seasonal variation affected the composition of the soil bacterial communities. After one year of burning, an increase in bacterial richness was observed, while the diversity of the bacterial communities reverted to pre-burning levels.

Stability of soil bacteria in undisturbed soil and continuous maize cultivation in Northern Thailand.

Rotational shifting cultivation (RSC) in Northern Thailand serves the dual purpose of ensuring food security and meeting economic goals through maize cultivation. However, the research question remains: Does the dynamics of soil bacterial communities differ between maize monoculture and RSC fields with continuous fallow throughout the season? Therefore, the objective of this study was to investigate and compare the variation of soil bacterial communities in maize monoculture and fallow RSC fields. A continuous 5-year fallow field (undisturbed soil; CF-5Y) and a continuous 5-year maize cultivation field (M-5Y) in Mae Chaem District, Chiang Mai Province, Northern Thailand, were selected due to their similarities in microclimate, topography, and the 5-year duration of different field activities. Over the span of a year, we collected soil samples from the surface layer (0-2 cm depth) at both sites. These collections occurred at 3-month intervals, starting from March 2022 (summer season) and followed by June (rainy season), September (rainy season), December (winter season), and March 2023 (summer season). Soil bacterial diversity and composition were analyzed using 16S rRNA gene-based metagenomic analysis. The results found that undisturbed soil over a 5-year period exhibited more stability in the richness and diversity of bacteria across seasons compared with M-5Y. Notably, fertilizer application and tillage practices in M-5Y can enhance both the diversity and richness of soil bacteria. In terms of bacterial abundance, Proteobacteria prevailed in CF-5Y, while Actinobacteria dominated in M-5Y. At the genus level, Candidatus Udaeobacter dominated during the summer and winter seasons in both CF-5Y and M-5Y sites. Interestingly, during the rainy season, the dominant genus shifted to Bacillus in both CF-5Y and M-5Y fields. The soil bacterial community in M-5Y was strongly influenced by organic matter (OM) and organic carbon (OC). In contrast, in CF-5Y, there was no correlation between soil properties and the soil bacterial community, likely due to the lower variation in soil properties across seasons. ß-Glucosidase was the dominant enzyme in both CF-5Y and M-5Y sites, and it showed a positive correlation with OM and OC. Further studies should continue to investigate soil bacteria dynamics, considering the changes in land management practices.

Effect of Rice Straw and Stubble Burning on Soil Physicochemical Properties and Bacterial Communities in Central Thailand.

Rice straw and stubble burning is widely practiced to clear fields for new crops. However, questions remain about the effects of fire on soil bacterial communities and soil properties in paddy fields. Here, five adjacent farmed fields were investigated in central Thailand to assess changes in soil bacterial communities and soil properties after burning. Samples of soil prior to burning, immediately after burning, and 1 year after burning were obtained from depths of 0 to 5 cm. The results showed that the pH, electrical conductivity, NH4-N, total nitrogen, and soil nutrients (available P, K, Ca, and Mg) significantly increased immediately after burning due to an increased ash content in the soil, whereas NO3-N decreased significantly. However, these values returned to the initial values. Chloroflexi were the dominant bacteria, followed by Actinobacteria and Proteobacteria. At 1 year after burning, Chloroflexi abundance decreased remarkably, whereas Actinobacteria, Proteobacteria, Verrucomicrobia, and Gemmatimonadetes abundances significantly increased. Bacillus, HSB OF53-F07, Conexibacter, and Acidothermus abundances increased immediately after burning, but were lower 1 year after burning. These bacteria may be highly resistant to heat, but grow slowly. Anaeromyxobacter and Candidatus Udaeobacter dominated 1 year after burning, most likely because of their rapid growth and the fact that they occupy areas with increased soil nutrient levels after fires. Amidase, cellulase, and chitinase levels increased with increased organic matter levels, whereas ß-glucosidase, chitinase, and urease levels positively correlated with the soil total nitrogen level. Although clay and soil moisture strongly correlated with the soil bacterial community's composition, negative correlations were found for ß-glucosidase, chitinase, and urease. In this study, rice straw and standing stubble were burnt under high soil moisture and within a very short time, suggesting that the fire was not severe enough to raise the soil temperature and change the soil microbial community immediately after burning. However, changes in soil properties due to ash significantly increased the diversity indices, which was noticeable 1 year after burning.

Environmental, Human and Ecotoxicological Impacts of Different Rice Cultivation Systems in Northern Thailand.

Sustainable practices in rice cultivation require effective farming management concerning environmental and human health impacts. In this study, three rice cultivation systems, namely low-land, upland, and terraced rice in the Mae Chaem District, Chiang Mai Province, were assessed and the carbon footprint (CF), water footprint (WF), and human and ecotoxicological impacts were compared from pesticide application. The results showed that the highest CF intensity was observed in terraced rice with 1.15 kg CO2eq kg-1 rice yield, followed by lowland rice (1.02 kg CO2eq kg-1 rice yield) and upland rice (0.17 kg CO2eq kg-1 rice yield) fields. Moreover, lowland rice cultivation generated the highest total WF with 1701.6 m3 ton-1, followed by terraced rice (1422.1 m3 ton-1) and upland rice (1283.2 m3 ton-1). The lowland rice fields had the most impact on human health and freshwater ecotoxicity, followed by the terraced and upland rice cultivation systems. The results also showed that most of the pesticides remaining in soils were chlorpyrifos (98.88%), butachlor (96.94%), and fipronil (95.33%), respectively. The substances with the greatest distributions in freshwater were acephate (56.74%), glyphosate (50.90%), and metaldehyde (45.65%), respectively. This study indicated that, with more agricultural inputs, higher CF, WF, human health impacts, and freshwater ecotoxicity were generated. Although the use of pesticides in the study areas did not exceed the recommendations on the packaging, glyphosate and chlorpyrifos are restricted in Thailand, so it is necessary to monitor their use due to their long-term health effects.

Soil Microbial Diversity and Community Composition in Rice-Fish Co-Culture and Rice Monoculture Farming System.

Soil microorganisms play an important role in determining nutrient cycling. The integration of fish into rice fields can influence the diversity and structural composition of soil microbial communities. However, regarding the rice-fish co-culture (RF) farming system in Thailand, the study of the diversity and composition of soil microbes is still limited. Here, we aim to compare the microbial diversity, community composition, and functional structure of the bacterial communities between RF and rice monoculture (MC) farming systems and identify the environmental factors shaping bacterial community composition. Bacterial taxonomy was observed using 16s rRNA gene amplicon sequencing, and the functional structures of the bacterial communities were predicted based on their taxonomy and sequences. The results showed that soil organic carbon, total nitrogen (TN), organic matter, available phosphorous, and clay content were significantly higher in RF than in MC. The most dominant taxa across both paddy rice fields belonged to Actinobacteria, Chloroflexi, Proteobacteria, Acidobacteria, and Planctomycetes. The taxa Nitrosporae, Rokubacteria, GAL15, and Elusimicrobia were significantly different between both rice fields. At the genus level, Bacillus, Anaeromyxobacter, and HSB OF53-F07 were the predominant genera in both rice fields. The most abundant genus in MC was Anaeromyxobacter, whereas RF belonged to Bacillus. The community composition in MC was positively correlated with magnesium and sand content, while in RF was positively correlated with pH, TN, and clay content. Nitrogen fixation, aromatic compound degradation, and hydrocarbon degradation were more abundant in RF, while cellulolysis, nitrification, ureolysis, and phototrophy functional groups were more abundant in MC. The enzymes involved in paddy soil ecosystems included phosphatase, ß-glucosidase, cellulase, and urease. These results provide novel insights into integrated fish in the paddy field as an efficient agricultural development strategy for enhancing soil microorganisms that increase soil fertility.

Effects of fire on soil organic carbon, soil total nitrogen, and soil properties under rotational shifting cultivation in northern Thailand.

Fire has been used for land clearing under rotational shifting cultivation (RSC) in Northern Thailand for a long time. However, the effects of fire on soil organic carbon (SOC), soil total nitrogen (STN), and soil properties are not well understood. We determined SOC, STN, and soil properties of the topsoil layer (0-30 cm) along a fallow chronosequence under RSC and assessed how fire affects SOC, STN, and soil properties. Eight fields at Ban Mae Pok, Mae Chaem District, Chiang Mai Province, Northern Thailand, were investigated. The levels of SOC, STN, and soil properties were observed at three time points: pre-burning, post-burning (5 minutes after burning), and post-harvest (nine months after burning). The highest SOC and STN stocks, organic matter (OM), and clay content were observed for the longest fallow period (7 years fallow), whereas the shortest period (1 year fallow) resulted in the lowest SOC and STN stocks. Fire caused no significant changes in SOC, STN, and some soil properties (soil texture, available P, exchangeable K, exchangeable Ca, exchangeable Mg, bulk density, and OM) because of the low fire intensity and short fire duration. Only pH and electrical conductivity were significantly increased (p ≤ 0.05) after burning due to the demobilization of base cations in burnt vegetation and incorporation into the soil with ashes and wood charcoal. Although fire may still be necessary for RSC, maintaining the fire intensity below 380 °C to reduce SOC losses and appropriate post-fire management strategies to reduce STN losses are crucial.

Climate change impact on major crop yield and water footprint under CMIP6 climate projections in repeated drought and flood areas in Thailand.

Understanding crop yield and water requirements in response to the future climate at the local scale is essential to develop more precise and appropriate adaptation strategies. From this perspective, repeated drought and flood events in the lower north of Thailand were investigated. The objectives of the study were to evaluate the impact of climate change on major crop yields and the water footprint (WF). Five global circulation model datasets from the sixth phase of the Coupled Model Intercomparison Project (CMIP6), known as Shared Socioeconomic Pathways (SSPs), were selected. Three future periods: near (2015-2039), mid (2040-2069), and far future (2070-2100) under SSP245 and SSP585 scenarios were used to predict the major crop yields and WF changes in the future. The precipitation and maximum and minimum temperatures were projected to increase in all periods under both scenarios. Rice yields in irrigated areas were predicted to rise gradually over the three projection periods under SSP245 but decline in mid and far-future periods under SSP585. There was a predicted reduction of first and second rice crop yields by -6.0% to -17.7% under SSP585. Fortunately, those rice yields were expected to increase in the near-future period under SSP245 by 3.0% to 4.3%. Growing maize, soybean, or mung bean instead of a second rice crop will have a less negative impact on future climate change. Changing from growing rice to be planting maize twice per year and growing cassava had increased favorability in rain-fed areas. The WF changes in the future were associated with future crop yield changes; therefore, the decrease in WFs was due to an increase in crop yield and vice-versa. The total WFs of maize, soybean, mung bean, and cassava production were roughly half that of rice production, indicating that these crops are suitable alternatives in the dry season.

Urban Agriculture in Thailand: Adoption Factors and Communication Guidelines to Promote Long-Term Practice.

The purpose of this study is to clarify influencing factors on the adoption and continuing practice of urban agriculture, and to propose communication guidelines to encourage more adoption and long-term practice. The psychological theories of the Theory of Reasoned Action (TRA), the Theory of Planned Behavior (TPB), and the Health Belief Model (HBM) were integrated to explain people's behavior. Both quantitative and qualitative approaches were employed with 325 villagers of 13 communities in seven provinces of Thailand. The following techniques and instruments were used: a statistical analysis crosstab, stepwise multiple linear regression, one-way ANOVA, multinomial logistic regression, decision tree analysis, and descriptive content analysis using QDA lite miner software. The key results clearly show that attitude, perceived benefits, and perceived readiness were a significantly positive influence on those who adopted urban agriculture. Key drivers to villagers' intention to continue practicing urban agriculture for the initiator group who own agricultural land were perceived to be behavioral control and social norm, while perceived readiness and communication played a crucial role for other groups to continue practicing urban agriculture. Communication guidelines to promote long-term urban agriculture practice can be designed based on the EAST framework, by making it easy, attractive, social, and timely, and through the Critical Participatory Action Research process.

Carbon footprint and predicting the impact of climate change on carbon sequestration ecosystem services of organic rice farming and conventional rice farming: A case study in Phichit province, Thailand.

Organic rice farming is a sustainable rice cultivation system that eliminates chemical inputs and has the potential to reduce environmental impacts. This study aims to: 1) evaluate and compare the carbon footprint intensity and the value of carbon sequestration ecosystem services (VCSES) between organic rice farming (OF) and conventional rice farming (CF) and 2) estimate the impact of climate change on soil organic carbon (SOC), rice yield, and VCSES of two farming types in Phichit province, Thailand. The results showed that the carbon footprint intensity in OF and CF were significantly different with -0.13 and 0.82 kg CO2eq kg-1 rice yield, respectively. The differences in SOC stocks (ΔSOCS) were more significant in OF with the increase of 1107.6 kg C ha-1 year-1 (4061.2 kg CO2eq ha-1 year-1), while the ΔSOCS value in CF was 625 kg C ha-1 year-1 (2291.7 kg CO2eq ha-1 year-1). The VCSES in OF (541,196 US$ ha-1 year-1) was nearly two times higher than in CF (305,388 US$ ha-1 year-1). Under future climate change, rice yields of both farming types are expected to increase under Representative Concentration Pathway (RCP) 2.6, RCP4.5, and RCP6.0, and it will decline under RCP8.5. The SOC and VCSES values are predicted to increase, except under RCP8.5. The dramatic declines can be found from the near future (2020-2039) to the very far future (2080-2099) period. Our finding indicates that even though climate change will have negative effects on SOC and VCSES, the OF will have less impact compared with CF.

Assessment of climate change impact on rice yield and water footprint of large-scale and individual farming in Thailand.

Large-scale farming (participation in large-scale agricultural extension program) and individual farming (no participation) are two farming management practices of rice cultivation in Thailand, both of which cause significant water consumption and degradation and are vulnerable to climate change. However, given that climate change will influence both grain yield and water resource availability, it is not fully understood which type of farming management practice is more adaptive to climate change. This study aims to evaluate the adaptation capabilities of large-scale and individual farming by simulating rice yield changes under future climatic conditions and estimating the climate change impact on the water footprint (WF) of rice production. Rice management practices were obtained from large-scale and individual farming. Five General Circulation Models of RCP4.5 and RCP8.5 scenarios under four future time periods were used as future climate projections. Simulation results show a remarkable increase in rice yield of individual and large-scale farming under RCP4.5, ranging from 1.3 to 29.8% and 2.0 to 30.8%, respectively, whereas it fluctuates from 11.7 to -29.0% and 8.3 to -20.8% under RCP8.5 for individual and large-scale farming, respectively. The projected total WF of rice production under RCP4.5 will decline, ranging from -10.0 to -43.0% and -0.5 to -67.0% for individual and large-scale farming, respectively. Conversely, the RCP8.5 shows a fluctuation in projected total WF of -26.5 to 63.3% and -51.1 to 60.0% for individual and large-scale farming, respectively. The total WF, mainly grey WF, in large-scale farming is lower than in individual farming. The increase of rice yield under RCP4.5 is due to an increment of temperature and precipitation, resulting in a decrease of the total WF and vice versa for RCP8.5. The large-scale farms are highlighted as adopting appropriate management practices for rice production in which they can maintain rice yield and reduce grey WF.