Ultrasonic microrheology for ex vivo skin explants monitoring: A proof of concept.

As an answer to alternative non-animal testing, biosensors dedicated to the ex vivo skin explants monitoring are a challenge to study physiological-like behavior and optimize new topical products. Because of the skin viscoelastic behavior, mechanical tests are commonly based on macroscopic measurement and give global descriptors of its state. Other techniques, including photoacoustic ones, are more focused on the molecular scale. There is a gap to fill in the mesoscopic range to get information about the microstructure of the skin. This article presents the proof-of-concept of a biosensor coupling a thickness shear-mode transducer with human skin explants kept in life-like state for a week. Thanks to a multifrequency analysis of the transducer impedance, this biosensor is able to monitor the viscoelastic properties of the skin. To extract the complex shear modulus and the microstructural evolutions, a mechanical model based on fractional calculus is used. As a preliminary results, the sensitivity of the sensor to probe the skin viscoelasticity in lifelike state and the impact of its culture medium are presented. A suitable microstructural coefficient is also extracted in order to identify mechanical breaches in the skin barrier after the application of peeling products.

Non-Contact Radiofrequency Inductive Sensor for the Dielectric Characterization of Burn Depth in Organic Tissues.

A flat circular transmission line-based 300 MHz resonator was implemented for the non-contact assessment of burn depths in biological tissues. Used as a transmit-and-receive sensor, it was placed at a 2 mm distance from organic material test samples (pork fillet samples) which were previously burned on their surface in various heating conditions involving different temperatures, durations, and procedures. Data extracted from the sensor by means of a distant monitoring coil were found to clearly correlate with the depth of burn observed in the tissue samples (up to 40% sensor output changes for a 7 mm burn depth) and with the heating conditions (around 5% sensor output changes observed in samples burned with identical heating procedures but at two different temperatures-75 °C and 150 °C-and around 40% sensor output changes observed between samples heated at the same temperature but with different heating procedures). These results open the way for the development of easy-to-implement assessment and monitoring techniques for burns, e.g., integrated in wearable medical dressing-like monitoring devices.

Experimental ultrasonic characterization of polyester-based materials for cultural heritage applications.

For several years, the Réunion des musées nationaux - Grand-Palais has produced polyester resin reproductions in order to replace marble sculptures that have weakened by outdoor exposure. These objects are made of a complex multilayered polyester composite material including reinforcements to ensure the mechanical strength of the final structure and mineral fillers that allow to imitate the original aesthetics. However, the final structure also weakens because of constant outdoor exposure and ageing. This observation leads today to conduct research related to the structural health monitoring of reproductions for preventive conservation of cultural heritage. This paper presents a nondestructive technique to study the properties of the composite material used to produce reproductions of marble sculptures. Firstly, classical ultrasonic contact measurements were performed to estimate bulk properties and Rayleigh wave velocity. Secondly, experimental Rayleigh wave was measured using contact and laser vibrometry methods. The results show the potential of using ultrasonic surface wave propagation and laser vibrometry method to develop a minimum contact technique to study these polyester-based materials. The maximum relative uncertainty with respect to the expected theoretical Rayleigh wave velocity was close to 12%.

Study of the bending modes in circular quartz resonators.

An experimental and theoretical study of bending modes in a partially electroded circular piezoelectric quartz (AT-cut) with free edge is presented. The quartz is excited by a voltage pulse applied on the electrodes, and its surface is scanned by a laser vibrometer that measures the out-of-plane displacements. The classical theory of bending of thin disks is used to describe the flexural modes at frequencies lower than the first thickness shear resonance (6 MHz). A fairly good agreement is found between experimental and theoretical results for the forced mode shapes and for the resonance frequencies. However, it appears that the two springs used to maintain the disk in position introduce extra clamping conditions. Several source shapes were studied, among which a collection of an arbitrary number of forces is particularly useful. The two-dimensional wavenumber representation shows the presence of anisotropy related to the crystallographic axes at higher frequencies, which is not predicted by the model. The experimental phase velocities are compared to those given by the classical theory of disks and to those of Lamb A(0) mode. This study confirms the correspondence at low frequencies between the A(0) mode and the bending eigenmodes of a disk with finite size.

3D Gabor analysis of transient waves propagating along an AT cut quartz disk.

Laser detection methods allow the investigation of ultrasonic transient phenomena in both space and time dimensions. Used for the experimental investigation of surface wave propagation along a 2D surface, laser ultrasonic leads to three dimensional (3D) space-time signal collections. The classical high resolution signal processing methods or 3D Fourier Transforms can be used in order to extract the wave propagation information, however these methods are not adapted for identifying where and when the waves are generated. In order to quantify these transient aspects in the space-time-wave number-frequency domains, the 3D Gabor transform is introduced. The 3D Gabor transform properties are presented. The potential of the 3D Gabor for the identification of the local and transient complex wave numbers is illustrated on the propagation of surface waves on a piezoelectric quartz (AT cut, 6 MHz). In this experimental study, the quartz is excited by a voltage pulse and the quartz surface is scanned by a laser vibrometer. The 3D Gabor analysis shows that the circular electrodes borders generate anti-phase surface waves that propagates outside the electrodes, with a strong energy contribution in the low frequency domain (<1 MHz). The transient analysis also points out, for higher frequencies, where the surface waves are generated and how they propagate with respect of both to the geometry of the electrodes and the crystallographic axis of the quartz. These results confirm the theoretical modal analysis and provide new knowledge about the key role played by the electrodes border. This will allow the optimization of the electrodes shape in order to design low frequency Lamb wave sensors.

Laser ultrasonic analysis of normal modes generated by a voltage pulse on an AT quartz sensor.

Laser ultrasonic detection is a versatile and highly sensitive tool for the observation of surface waves. In the following study, laser ultrasonic detection is used for the experimental study of spurious normal vibration modes of a disk quartz sensor excited by a voltage pulse. The AT cut crystal (cut of the crystal relative to the the main crystallographic axis is 35.25 degrees) is optimal for generating mainly thickness-shear vibrations (central frequency 6 MHz) on the quartz surface. However, resulting from shear-to-longitudinal and shear-to-surface mode conversion, and from the weak coupling with the other crystallographic axes, other modes (thickness-compressional and bending modes) are always present in the plate response. Since the laser vibrometer is sensitive to normal displacements, the laser investigation shows waves that can be considered as unwanted for the AT quartz used as a shear sensor. The scanned three dimensional (3D) amplitude-space-time signals are carefully analysed using their representation in three dual Fourier domains (space-time, wave number-frequency). Results on the transient analysis of the waves, the normal bending modes and the dispersion curves are shown.

Chirp-Z analysis for sol-gel transition monitoring.

Gelation is a complex reaction that transforms a liquid medium into a solid one: the gel. In gel state, some gel materials (DMAP) have the singular property to ring in an audible frequency range when a pulse is applied. Before the gelation point, there is no transmission of slow waves observed; after the gelation point, the speed of sound in the gel rapidly increases from 0.1 to 10 m/s. The time evolution of the speed of sound can be measured, in frequency domain, by following the frequency spacing of the resonance peaks from the Synchronous Detection (SD) measurement method. Unfortunately, due to a constant frequency sampling rate, the relative error for low speeds (0.1 m/s) is 100%. In order to maintain a low constant relative error, in the whole speed time evolution range, Chirp-Z Transform (CZT) is used. This operation transforms a time variant signal to a time invariant one using only a time dependant stretching factor (S). In the frequency domain, the CZT enables us to stretch each collected spectrum from time signals. The blind identification of the S factor gives us the complete time evolution law of the speed of sound. Moreover, this method proves that the frequency bandwidth follows the same time law. These results point out that the minimum wavelength stays constant and that it only depends on the gel.