Isolation and Characterization of a Serratia rubidaea from a Shallow Water Hydrothermal Vent.

Microbial life present in the marine environment has to be able to adapt to rapidly changing and often extreme conditions. This makes these organisms a putative source of commercially interesting compounds since adaptation provides different biochemical routes from those found in their terrestrial counterparts. In this work, the goal was the identification of a marine bacterium isolated from a sample taken at a shallow water hydrothermal vent and of its red product. Genomic, lipidomic, and biochemical approaches were used simultaneously, and the bacterium was identified as Serratia rubidaea. A high-throughput screening strategy was used to assess the best physico-chemical conditions permitting both cell growth and production of the red product. The fatty acid composition of the microbial cells was studied to assess adaptation at the lipid level under stressful conditions, whilst several state-of-the-art techniques, such as DSC, FTIR, NMR, and Ultra-High Resolution Qq-Time-of-Flight mass spectrometry, were used to characterize the structure of the pigment. We hypothesize that the pigment, which could be produced by the cells up to 62 °C, is prodigiosin linked to an aliphatic compound that acts as an anchor to keep it close to the cells in the marine environment.

Essential oils of Origanum vulgare L. subsp. glandulosum (Desf.) Ietswaart from Tunisia: chemical composition and antioxidant activity.

BACKGROUND: Characterisation of the essential oils from O. glandulosum collected in three locations of Tunisia, chemical composition and the evaluation of their antioxidant activities were carried out. RESULTS: The essential oils from Origanum vulgare L. subsp. glandulosum (Desf.) Ietswaart collected from three localities of north Tunisia-Krib, Bargou and Nefza-were obtained in yields of 2.5, 3.0 and 4.6% (v/w), respectively. The essential oils were analysed by GC and GC/MS and assayed for their total phenolics content, by the Folin-Ciocalteu method, and antioxidant effectiveness, using the 2,2-diphenyl-1-picrylhydrazil (DPPH) radical scavenging assay. The main components of these essential oils, from Nefza, Bargou and Krib, were p-cymene (36%, 40% and 46%), thymol (32%, 39% and 18%), gamma-terpinene (24%, 12% and 16%) and carvacrol (2%, 2% and 15%), respectively). The ability to scavenge the DPPH radicals, expressed by IC(50), ranged from 59 to 80 mg L(-1). The total phenolic content, expressed in gallic acid equivalent (GAE) g kg(-1) dry weight, varied from 9.37 to 17.70 g kg(-1) dw. CONCLUSIONS: A correlation was identified between the total phenolic content of the essential oils and DPPH radical scavenger capacity. The occurrence of a p-cymene chemotype of O. glandulosum in the northern region of Tunisia is demonstrated.

Sobrevida de um grande queimado: um novo enfoque terapêutico / Survival of severely burned patient: a new therapeutic approah

Os autores sugerem uma nova abordagem terapêutica do paciente grande queimado, reunindo tudo o que existe de mais moderno públicado na literatura internacional. Para ilustrar a importância deste novo enfoque terapêutico, apresentamos um caso clínico. Neste trabalho discutimos a importância da escovaçäo e intensa degermaçäo da área queimada sob hipnoanalgesia, do uso de antioxidantes e de soluçöes hipertônicas. E enfocada as alteraçöes hemodinâmicas, metabólicas e infecciosas.

Sobrevida de paciente com lesäo térmica maciça; um novo enfoque terapêutico / Survival of a severely burned patient; a new therapeutic approach

Os autores apresentam o caso de um paciente que sobreviveu a um extenso trauma térmico. Sugerimos nova abordagem terapêutica, reunindo o que existe de mais moderno publicado na literatura internacional. Neste relato discutimos a importância da escovaçäo e de intensa degermaçäo da área queimada sob hipnoanalgesia; tecemos também consideraçöes sobre as alteraçöes metabólicas e infecciosas