PCR as a confirmatory test for human immunodeficiency virus type 1 infection in individuals with indeterminate western blot (immunoblot) profiles.

A combination of two PCR systems which amplify human immunodeficiency virus type 1 (HIV-1) gag and pol DNA was used to elucidate the HIV-1 infection status of a group of individuals screening positive for anti-HIV but having indeterminate Western blot (immunoblot) profiles. The results obtained suggest PCR could be used to resolve such cases.