RESUMO
A novel, sensitive high-performance liquid chromatographic method, making use of coulometric detection, for the estimation of mebendazole and its metabolites in the sera of eight hydatidosis patients was devised. Recovery rates, precision, accuracy and sensitivity for each compound are reported and compared with those of the previously published methods.
Assuntos
Mebendazol/análogos & derivados , Mebendazol/sangue , Biotransformação , Cromatografia Líquida de Alta Pressão/métodos , Equinococose/tratamento farmacológico , Eletroquímica/métodos , Humanos , Indicadores e Reagentes , Espectroscopia de Ressonância Magnética/métodos , Espectrometria de Massas/métodos , Mebendazol/uso terapêutico , Microquímica , Espectrofotometria Ultravioleta/métodosRESUMO
Under the conditions described for alkaline hydrolysis of reserpine and rescinnamine in absolute and aqueous methanol, and after esterification (with diazomethane) of the resulting acid fraction, methyl 3,4,5-trimethoxybenzoate was quantitatively recovered, whereas methyl trans-3,4,5-trimethoxycinnamate, in normal lighting conditions, was either partly isomerized to methyl cis-trimethoxycinnamate or formed an adduct with a molecule of methanol, yielding methyl 3-methoxy-3-(3,4,5-trimethoxyphenyl)propionate. The structures of the products were established by synthesis, nuclear magnetic resonance studies and mass spectrometry. This investigation of the hydrolytic conditions allowed a reliable and rapid gas chromatographic determination of reserpine and/or rescinnamine in amounts down to 500 and 2000 mug, respectively, to be devised.
