Molecular characterization of Fasciola samples, collected from different host species coming from the endemic area of Turkey.

Economical animal breeding programs are important for achieving maximum gain, and any factors resulting in economical loss should be minimized or eliminated. An organism of concern is the liver fluke, Fasciola hepatica, which causes decreased yield and even death in sheep and dairy cattle. In an effort to eliminate or minimize the detrimental effect of this parasite in animals, it is important to understand the genetic diversity within the liver flukes and the relationship between this parasite and the host in the particular geographic area. The aim of this study was to explore genetic diversity by analyzing the mitochondrial ND1 and cyt b genes and Ribosomal ITS1-2 regions. With these analyses, the individual differences, the host animal differences and combined effects of these factors on genetic relationships have been determined.

[Cysticercus bovis in Turkey and its importance from the public health aspect]. / Türkiye'de Cysticercus bovis ve halk sagligi yönünden önemi.

This study was conducted in order to compare the different regions according to the literature on the prevalence of bovine cysticercosis and T. saginata in Turkey. Bovine cysticercosis and T. saginata status were evaluated retrospectively. The distribution of the data obtained according to provinces and regions were showed in the Table and the minumum / maximum values of this data in different regions in the Figure. The data obtained through the literature showed that the prevalence of C. bovis and T. saginata infections are parallel in the same region. The higher prevalence of both C. bovis and T. saginata infections was determined in the Southeastern Anatolia, Eastern Anatolia and Central Anatolia regions respectively.

[Cystic echinococcosis in the Mersin province (119 cases)]. / Mersin ili'nde kistik ekinokokkozis (119 olgu).

OBJECTIVE: This study was made to emphasize the importance of cystic echinococcosis in the Mersin province and to contribute to informarion regarding the geographic distribution of infection in Turkey. METHODS: Seven different pathology laboratory records in the Mersin province between the period 2011-2012 were evaluated according to age, sex and organ involvement. RESULTS: In study period, a total of 119 cases of cystic echinococcosis were determined. Male patients constituted 52.94% of the cases, and female, 47.05%. The age distribution of these cases was between 5 and 76 years. Most of the cases appeared in 41-50 (22.68%) year old patients. The most common localizations for cystic echinococcosis was determined in the liver (n: 53, 44.53%) and lung (n: 39, 32.77%), followed by the kidney (n: 4, 3.36%), spleen (n: 3, 2.52%) and gall bladder (n: 3, 2.52%). In 3 cases (2.52%), multiorgan involvement was seen. CONCLUSION: Cystic echinococcosis is an important health problem in Turkey. The stray dogs, which play an important role in the contagiousness of the disease, should be kept under control and treated. Also, slaughtering of animals must be preformed under the supervision of veterinary surgeons and destruction of infected organs should be carried out under proper conditions in slaughterhouses.

[Cysticercus bovis in slaughtered cattle in the Afyonkarahisar and Burdur provinces and its importance from the point of view of public health]. / Afyonkarahisar ve Burdur illerinde kesilen sigirlarda cysticercus bovis ve halk sagligi yönünden önemi.

OBJECTIVE: This study was conducted to determine the current status of bovine cysticercosis and human taeniosis in two provinces (Afyonkarahisar and Burdur in the western part of Turkey between October 2009 and October 2011). METHODS: In the study period, a total of 1684 cattle and 7644 human stool samples were examined for Cysticercus bovis, and Taeniosis, respectively. Carcasses of animals were subjected to routine meat inspection. Stool samples of humans were analysed macroscopically and microscopically including direct and formaldehyde-ether sedimentation methods. RESULTS: C. bovis was determined in 0.24% of cattle by postmortem examination. The prevalence of infection was found to be 0.46% in Afyonkarahisar and 0.09% in Burdur. Taenia sp. eggs were shown in 0.1% of the human stool samples. The infection rate was detected as 0.1% and 0.09% in Afyonkarahisar and Burdur, respectively. CONCLUSION: This study is characterized as a preliminary investigation which offers useful information on a public health level within a short time at a low cost. Results obtained from this study cannot be generalized to study areas in epidemiological terms, but they are useful data for the relevant geography.

The acute effects of single-dose orally administered doramectin, eprinomectin and selamectin on natural infections of Syphacia muris in rats.

This study was designed to determine the acute effects of a single-dose of orally administered doramectin, eprinomectin and selamectin on Syphacia muris infection in rats. Rats, naturally infected with S. muris, were divided into four groups: three different treatment groups (n=7) and one positive control (n=7). Cellophane tape preparations were obtained from the treated rats on day 0 pre-treatment and on days 2, 4 and 6 post-treatment. Syphacia sp. eggs were counted. Eprinomectin was found to be 100% effective in eliminating eggs on two post-treatment. However when egg counts on day 6 post-treatment were compared with pre-treatment egg counts, doramectin and selamectin were found to be 99.32 and 98.77% effective in eliminating eggs, respectively. On day 7 post-treatment, blood samples were obtained from all groups, and then the rats were necropsied. Doramectin, eprinomectin and selamectin were found to be 100% effective in eliminating adult S. muris, when compared with the positive control group.

Prevalence of cystic echinococcosis in slaughtered cattle in Afyonkarahisar.

This study was carried out in 1001 cattle that were slaughtered in 3 abattoirs in the Afyonkarahisar district of western Turkey. Abattoirs were visited twice a week and internal organs of the cattle were examined for cystic echinococcosis (CE). The number of organs discarded because of CE was recorded. CE was found in 295 (29.47%) out of 1001 cattle studied. CE was found in 113 (44.31%) out of 255 cows and 182 (24.39%) out of 746 bulls. This difference was statistically significant (p < 0.001). CE was observed only in liver of 130 (44.06%) cattle, only in lungs of 91 (30.85%); only in hearts of 2 (0.68%) and only in the kidney of 1 (0.34%); in both livers and lungs of 70 (23.73%), and in both hearts and lungs of 1 (0.34%). Also, the rate of CE was significantly higher in cows than bulls in terms of organ predilection (p < 0.001). Forty three livers were completely discarded along with half of 18 livers, a quarter of 37 livers and the remaining livers in various degrees. Forty four lungs were completely destroyed along with half of 13 and a quarter of 21 whereas 3 hearts and one kidney were completely destroyed. The fertility rate of cysts was found to be 5.42%. In this study the prevalence of CE in cattle in Afyonkarahisar district has been documented and possible economic losses due to CE were emphasized.

Efficacy of eprinomectin against Toxacara canis in dogs.

This study was made to investigate efficacy of eprinomectin against to Toxocara canis in dogs. In the study, 20 stray dogs naturally infected with T. canis were divided into two groups as treatment (ten dogs) and control (ten dogs). Eprinomectin (100 microg/kg, Eprinex 250 ml) was given to treatment group dogs orally, and eggs per gram were determined in the faeces on the day of pre-treatment and the second, fourth, sixth, eighth and tenth days of post-treatment. No side effects associated with nervous, respiratory, gastrointestinal systems and some haematological parameters were observed. In conclusion, eprinomectin was determined to be 100% effectual against T. canis.

[Examination of helminth contaminated wastewaters used for agricultural purposes in Afyonkarahisar]. / Afyonkarahisar'da tarimsal amaçli kullanilan atik sularin helmint kontaminasyonu yönünden incelenmesi.

This research was performed in order to examine helminth contaminated wastewaters used in agriculture in Afyonkarahisar. Ten rural areas were selected that used water for agricultural purposes from a creek contaminated with human and animal wastes. Between September 2004 and August 2005, these areas were visited once every two weeks, and a total of 240 water samples were collected. Out of untreated water samples, 32 samples (26.70%) had helminth eggs, whereas there were neither helminths nor developmental stages of helminths (p < 0.001) in treated water samples. Cestode eggs were seen in 10.00% of untreated water samples and nematode eggs were seen in 16.70%. Out of the helminth eggs, 13 (40.60%) were hookworm eggs; 6 (18.75%), Taenia spp.; 5 (5.60%), Ascaris lumbricoides; 3 (9.40%), Hymenolepis diminuta; 3 (9.40%), H. nana; and 2 (6.25%), Toxocara spp. In conclusion; the creek was found to be contaminated by helminths originating from human and animal wastes in Afyonkarahisar. This type of water presents a risk for human and animal health and contaminated waters, therefore, should be avoided in irrigation of vegetables and fruit consumed raw.

[The occurrence of gastrointestinal cestode and nematode infections in stray dogs in Afyonkarahisar and Eskisehir provinces]. / Afyonkarahisar ve Eskisehir illerindeki sokak köpeklerinde görülen gastrointestinal cestod ve nematod enfeksiyonlari.

This study was carried out to determine the prevalence of cestode and nematode infections in stray dogs in Afyonkarahisar and Eskisehir provinces. A total of 287 dogs (n=150 from Afyonkarahisar and n=137 from Eskisehir) of different ages and sexes living in animal shelters were used in this study. After the coprological examination, dogs were found to be infected with various gastrointestinal cestode and nematode species. It was found that rates of infection were 46% (69 out of 150) in Afyonkarahisar and 33.6% (46 out of 137) in Eskisehir provinces. Species responsible for the infection were the same in both provinces. In the Afyonkarahisar province 59.4% hookworms, 47.8% Toxascaris leonina, 36.2% Toxocara canis, 2.9% Dipylidium caninum and 2.9% Taenia spp. were detected. In the Eskisehir province 60.9% Toxascaris leonina , 47.8% Toxocara canis, 23.9% Taenia spp., 6.5% hookworms and 4.3% Dipylidium caninum were detected. It was concluded that in Afyonkarahisar and Eskisehir provinces, stray dogs might be infected with helminths which put humans in danger and necessary measures are needed.

Dirofilaria immitis infection in dogs: unusually located and unusual findings.

Clinical, blood, serum biochemistry, and parasitological assessments were performed on four hospitalized dogs, not in good general condition, in a study carried out to determine the prevalence of general parasitic infections. Hematological and biochemical parameters and electrocardiographic recording of the animals were determined during the general clinical examinations. Four dogs were indicated to have been infected with Dirofilaria immitis by using modified Knott's method, and the microfilarial density was determined. Mild to moderate anemia and decrease in sedimentation velocity were established in dogs. Differences in other hematological and biochemical values between the dogs were determined. Right axis deviation was determined in a dog. D. immitis was encountered in the right ventricle, bronchus, and the pericardial sac at the end of the necropsy. On the other hand, in the histopathological examinations, pulmonary adenocarcinoma deriving from bronchial epithelium was identified in a dog, and microfilaria was encountered in bronchial and bronchiolar lumens, interstitium of the lungs, and bile ducts. D. immitis has not been considered in the diagnosis of dogs with pneumonia and tumors. The requirement of parasitological examination with respect to D. immitis in the diagnosis of dogs with tumor or pneumonia has been considered vital.

[Gastrointestinal nematodes and their seasonal distribution in cattle raised in central Afyonkarahisar, Turkey]. / Afyonkarahisar il merkezinde yetistirilen sigirlarin mide bagirsak nematodlari ve mevsimsel dagilimlari.

This study was conducted during the period of September 2004-August 2005 in two villages with simple raising of cattle located in the center of the Afyonkarahisar province. Fecal samples collected from 985 cattle and the gastrointestinal tracts of 24 cattle were inspected. Strongyle types of eggs were observed in 26.39% of the fecal samples. The infection was found to be less prevalent in the spring (22%) and most prevalent in autumn (32.53%). The genus of larvae observed in fecal cultures were Haemonchus spp. 25.25%, Trichostrongylus spp. 23.71%, Nematodirus spp. 16.49%, Ostertagia spp. 10.30%, Cooperia spp. 8.76%, Bunostomum spp. 6.70%, Oesophagostomum spp. 6.18% and Chabertia ovina. 2.57%. H. contortus and Oesophagostomum venulosum (20.83%), O. radiatum (16.66%), Cooperia onchophora and Ostertagia ostertagi (8.3%), O. trifurcata, C. punctata and Chabertia ovina (4.16%) were found during inspections of the gastrointestinal tracts which were performed in order to determine the infective species. Based on these results, it was concluded that there are infections caused by gastrointestinal nematodes in this province but the severity of the infection is low and the seasons with highest infection risk are late spring and autumn.

[Paramphistomiasis and distomatosis in cattle in the Afyon province.]. / Afyon ili sigirlarinda paramphistomosis ve distomatosisin genel durumu.

In this study, livers, gall bladders, rumens and reticulums from 746 males and 255 females, making a total of 1001 slaughtered cattle, were examined in order to determine the prevalence of paramphistomiasis and distomatosis during the period of June 2003-November 2003 in the Afyon province. The infection rate of parasites was 13.6% (136 cattle) for Paramphistomidae spp., 4.6% (46 cattle) for Fasciola spp., 15.3% (153 cattle) for Dicrocoelium dendriticum and 1.4% (14 cattle) for either Fasciola spp. or D. dendriticum. The infection of males with paramphistomiasis and distomatosis was 3.05 times more than that in the females.