Seasonal Prevalence and Status of Anthelmintic Resistance of Goats' Gastrointestinal Nematodes, Mirab Abaya, Southern Ethiopia.

Goats are an important source of livelihood especially for smallholder communities. But gastrointestinal nematodosis is the greatest threats of goat production. A repeated cross-sectional and field experimental study design was conducted from December 2020 to August 2021 in Mirab Abaya district, with the aim of seasonal prevalence gastrointestinal nematode infection and assessment of anthelmintic resistance. A systematic random sampling strategy was used to select the study goats. The overall prevalence of gastrointestinal nematode infections of goats in the study area was 57.9% (95% CI: 54.4-61.4), of which 48.7% (95% CI: 43.7-53.7) and 67.2% (95% CI: 62.3-71.7) accounted to dry and wet seasons, respectively. The multivariable logistic regression analysis showed that season, age, sex, and flock size were significantly (p < 0.05) influenced the prevalence of gastrointestinal nematode infection of goats. The number of eggs per gram of faeces (EPG) was ranged from 100 to 2500. The overall mean egg per gram of faeces (EPG) was 461.1 ± 20.9. The mean EPG of wet season (532.7 ± 28.6) was higher than the dry season (362.3 ± 29.1). From faecal culture, 552 nematode larvae were recovered, and the most commonly identified nematode genera were Haemonchus (36.4%) that followed by Trichostrongylus (28.1%), Oesophagostomum (15.4%), Bunostomum (11.4), Teladorsagia (5.4%), and Strongyloides (3.0%) species. From the anthelmintic resistance test in the field, the percentage reduction and 95% confidence limit of albendazole were 96% (84.2-99.0) and 97.0% (84.6-99.4) in dry and wet seasons, respectively. Here, the lower limit of 95% CI was less than 90% both in dry and wet seasons; and hence, albendazole was suspected for resistance development by gastrointestinal nematode. From a pooled faecal culture that collected from albendazole-treated goats, Haemonchus species was recovered. Therefore, due attention shall be by animal health professionals in the area in the use of albendazole. Animal health extension work to create awareness of how anthelmintic is used is helpful in reducing the risk. Changing the type of anthelmintic drug after using for some period will minimize the risk of anthelmintic resistance development.

Prevalence, Risk Factors and Control Practices of Livestock Hirudiniasis in Mirab Abaya District, Southern Ethiopia.

Purpose: Hirudiniasis is a disease in people and animals who have been infested by blood sucking leeches. In Ethiopia, it is a neglected disease, causing significant economic loss in the livestock industry. In the study area, information on livestock Hirudiniasis is very limited. Therefore, this study was conducted to assess livestock owners' awareness, knowledge, and control practices toward leech infestation, and to estimate the prevalence and associated risk factors of livestock Hirudiniasis. Methods: A cross-sectional study was carried out from February to September 2022 on domestic animals randomly selected from livestock owners of Mirab Abaya district, Southern Ethiopia. In total, 384 domestic animals were sampled and examined for the presence of leech infestation. A questionnaire survey was carried out on 150 livestock owners. Results: This study discovered a 13.54% (52/384) overall prevalence of livestock Hirudiniasis in the study area and the highest prevalence was observed in cattle (22.14%), followed by sheep (10%), goats (8.65%), and equines (5%). Limnatis nilotica was the only leech species identified in the study area. The occurrence of livestock Hirudiniasis was significantly higher in the dry season (OR=3.16, p<0.05), around bodies of water (OR=10.88, p<0.01), in extensive production systems (OR=3.81, p<0.05), and in adult (OR=2.58, p<0.05) and poor body condition animals (OR=9, p<0.01). However, the species and sex of domestic animals were not significantly associated with Hirudiniasis. The questionnaire showed 61.33% and 35.33% of respondents had knowledge about leech infestations in domestic animals and its zoonotic impacts, respectively. Manual removal and traditional medicine are common control measures taken by livestock owners against Hirudiniasis. Conclusion: The present study showed that leeches were prevalent and significantly affects the health and productivity of livestock in the study area. Hence, cost-effective parasitic control measures need to be implemented to address the setback.

Detection of Antibodies Against Brucellosis and Associated Risk Factors in Cross Breed Dairy Cattle in Smallholder Farmers, Southern Ethiopia.

Background: A cross-sectional study was conducted in selected districts of Gamo zone, southern Ethiopia over a period of November 2019 and September 2020 to estimate seroprevalence and associated risk factors and assess knowledge and practices of smallholder farmers about bovine brucellosis. Two districts and four kebeles from each district were purposively selected, and individual animals were sampled using a simple random sampling technique. A total of 384 sera samples were collected, and concurrently, 236 dairy cattle owners were interviewed. The samples were tested for antibodies against Brucella using both Rose Bengal Plate test and Complement Fixation test following OIE standard protocol. Risk factors associated with bovine brucellosis were analyzed using univariate and multivariate logistic regressions. Results: The survey result has shown that 95% and 97% of the small holder farmers did not know the cause and symptoms of bovine brucellosis, respectively, and the majority have engaged in risky practices. Eight (2.08%) of the collected serum samples were positive with screening test (RBT) and only six (1.5%) were positive with confirmatory test (CFT). Multivariable logistic regression analysis showed a statistically significant association between herd sizes and the disease. The seropositivity of the disease is higher in small-sized herds followed by medium-sized herds. Conclusion: The seroprevalence of bovine brucellosis was found to be at a low percentage with confirmatory tests even if there was a presence of associated risk factors for the disease in the study area. Again, the results suggest that smallholder farmers have poor knowledge and risky practices, which expose them to the disease. Awareness creation about the disease is of paramount importance even if the prevalence was low in this serological study. The implementation of a test and slaughter program before the disease becomes widespread, along with the testing of new stock before introduction to the farms is recommended.

Glossina pallidipes Density and Trypanosome Infection Rate in Arba Minch Zuria District of Gamo Zone, Southern Ethiopia.

Background: African trypanosomosis is a disease of both animals and humans resulting from infection with parasitaemic protozoa of the genus Trypanosoma transmitted mainly by the tsetse flies (Glossina species). The disease has been reported in different parts of the country. However, information on the apparent density and trypanosome infection rates of the vectors is very limited in the Southern part of Ethiopia. Therefore, this study was conducted to estimate the apparent density, infection rate of trypanosomes in Glossina pallidipes, and the trypanosome species involved in Arba Minch Zuria district of Southern Ethiopia. Methods: A cross-sectional study was conducted from January to June 2018 in two purposely selected kebeles of Arbaminch Zuria district and in the escarpments of Nech Sar National Park of Southern Ethiopia. For entomological survey, a total of 40 standard NGU traps were deployed around the watering and grazing areas. A total of 300 fresh Glossina pallidipes were examined for trypanosome infection using a dissection procedure as described by the FAO Training manual for tsetse control personnel. Results: The study revealed the presence of only one Glossina species, known as Glossina pallidipes, and biting flies including Stomoxys and Tabanus. A total of 2176 flies were caught of which 1803 (82.86%) belong to Glossina pallidipes and the remaining 373 (17.14%) were biting flies. The overall apparent density of Glossina pallidipes and biting flies in the study area were 15.03 fly/trap/day (F/T/D) and 3.11 F/T/D, respectively. Relatively higher Glossina pallidipes and biting flies, respectively, were caught in a wood-grass land (15.87 F/T/D and 3.69 F/T/D) and riverine forest (15.13 F/T/D and 3.42 F/T/D) than bush land vegetation types (13.87 F/T/D and 1.76 F/T/D). The overall trypanosome infection rate of Glossina pallidipes was 17.67% (53/300). Two trypanosome species, namely, Trypanosoma congolense (66.04%) and Trypanosoma vivax (33.96%), were responsible for Glossina pallidipes infection in the study area. Trypanosome infection rate was significantly higher in female G. pallidipes than in male (OR = 3.39, 95%CI = 1.53-7.52). Significantly, higher trypanosome infection rate was observed in flies older than 20 days (OR = 2.5, 95%CI = 1.12-5.56) and in hunger stage 1 flies (OR = 14.24, 95%CI = 4.01-50.59). Glossina pallidipes infection was significantly higher in and around park grazing areas (OR = 3.41, 95%CI = 1.76-6.6) and wood-grass land vegetation type (OR = 2.7, 95%CI = 1.2-6.5). Conclusion: The current study revealed high apparent density and trypanosome infection in Glossina pallidipes in Arba Minch Zuria district of Southern Ethiopia. So, this study warrants the need for strengthening both vector and parasite control strategies in the study area.

Seroprevalence and associated risk factors for foot and mouth disease virus seropositivity in cattle in selected districts of Gamo zone, Southern Ethiopia.

Background: Foot and mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals, which hampers livestock production and productivity in Ethiopia. This cross-sectional study was conducted from January to December 2021 to estimate the seroprevalence of FMD in cattle and to assess farmers' knowledge about the disease in selected districts of the Gamo zone. Three districts and two kebeles (smallest administrative division) from each district were purposively sampled using a simple random sampling technique to select individual animals from each kebeles. A total of 384 sera samples were collected, and concurrently, 100 farmers were interviewed. The samples were tested for antibodies against nonstructural proteins of the FMD virus using a 3ABC enzyme-linked immunosorbent assay (ELISA). Univariable and multivariable logistic regressions were used to analyze FMD-associated risk factors. Result: The questionnaire survey result revealed that among the interviewed farmers, 66% of farmers had knowledge about the disease, and 28% of farmers reported having a case of FMD in at least one cattle in their farm in the previous 6 months. The overall seroprevalence of FMD in cattle was 26.8%. The multivariable logistic regression revealed that age, breed, and agroecology had a significant association with seropositivity. Higher seroprevalence (64.57%) was observed in lowland, followed by midland (9.30%) and highland (5.88%). Study animals from lowland areas were 9.26 times more likely to be seropositive (OR = 9.26, CI = 2.22-38.62) for FMD than highland animals. Also, adult animals were 9.01 times (OR = 9.01, CI = 3.18-25.53) more likely to be seropositive for the disease than young animals. The multivariable logistic regression revealed that crossbreeds have an 84.7% (OR = 0.153, CI = 0.028-0.82) lower likelihood to be seropositive to FMD than local breeds. Conclusion: This study result confirms that FMD is highly prevalent in the study area, and farmers' knowledge regarding disease transmission and vaccine availability is minimal. Hence the regional concerned bodies should implement FMD vaccination campaigns and create awareness for smallholder farmers regarding the disease transmission, FMD vaccine schedule, and vaccination importance.

Public Awareness, Prevalence and Potential Determinants of Bovine Tuberculosis in Selected Districts of Gamo Zone, Southern Ethiopia.

Purpose: Bovine tuberculosis (bTB) is infectious chronic disease of animals mainly caused by bacillus Mycobacterium bovis. It is known endemic disease of cattle in Ethiopia. In the current study sites, there is little information on bovine tuberculosis. Thus, this study was aimed to assess public awareness; and estimate prevalence and potential a risk factors of bovine tuberculosis. Methods: A cross-sectional study was conducted, November 2018 to May 2019, on dairy cattle randomly selected from smallholder farms of Arba Minch Zuria and Chencha districts. Comparative intradermal tuberculin test (CIDT) was used in the diagnosis of bTB in dairy animals. CIDT was administered to 221 dairy cattle. Questionnaire survey was performed on 110 smallholders to assess risk factors accountable for the event of bTB in human population. Results: 8.2% animal level prevalence of bTB was documented in the study sites. Dairy cattle with age of 4 to 8 eight years were 34% more likely to be affected by bTB less than 4 years age cattle (OR=1.34). However, factors like housing, body condition, herd size and parity were not significantly related with the event of bTB. The assessment of bTB awareness was shown 29.7% participants knew that cattle could be infected, and 13.4% of the respondents believed that it can spread from animal to human and vice versa. Besides, 66.1% of participants had habit for consumption of raw milk and soured milk products. Moreover, respondent's attitude about zoonoses and consequences of bTB was risky. Conclusion: Present study discloses that bTB is animal health problem affecting dairy cattle in Gamo zone, Southern Ethiopia. Based on the current findings, public awareness creation, bovine tuberculosis test and segregation strategy should be applied to minimize the public health hazards and risk factors for bovine origin tuberculosis.

Seasonal Patterns: Bovine Trypanosomosis, Glossina pallidipes Density, and Infection in Rift Valleys of Gamo Zone, Southern Ethiopia.

Bovine trypanosomosis is a parasitic disease causing serious economic losses in livestock productivity and agricultural development. The disease has been reported in different parts of Ethiopia. However, seasonal pattern of trypanosomosis, tsetse fly apparent density, and infection are very limited in the southern rift valley of the country, particularly in Gamo Zone. Therefore, the objective of this cross-sectional study design was to estimate seasonal prevalence of bovine trypanosomosis, assessing tsetse fly apparent density and its infection by trypanosomes. For the parasitological study, a total of 600 cattle (300 in each season) were sampled and assayed using the buffy coat technique. A total of 80 standard NGU traps were deployed around the watering and grazing areas for the entomological survey. An overall prevalence of trypanosomosis was 10.17% (61/600), of which 7.33% (22/300) and 13% (39/300) accounted for the dry and wet seasons, respectively. The prevalence of trypanosomosis was significantly higher during the wet season (OR = 2.47; p < 0.05), in black coat color (OR = 7.2, p < 0.05), and poor body-conditioned (OR = 3.15; p < 0.05) animals. Two species of trypanosomes, Trypanosoma congolense, 68.85% (42/61), and Trypanosoma vivax, 31.15% (19/61), were circulating in the area. The mean PCV value in infected animals (22.56 ± 4.61) was significantly lower than in non-infected animals (25.3 ± 4.75). Entomological result indicated that Glossina pallidipes (G. pallidipes) was the only species of tsetse found in the study area. Totally, 3,789 flies were caught of which 81.42% (3,085/3,789) belong to G. pallidipes and 18.58% (704/3,789) were other biting flies. The overall apparent density of G. pallidipes was 12.85 flies/trap/day (FTD). Relatively higher G. pallidipes/trap/day were caught in the wet season (13.64 F/T/D) than in the dry season (12.07F/T/D). Of the flies caught, 342 G. pallidipes were randomly selected and dissected. The overall proportion of G. pallidipes infection was 18.42% (63/342) of which 12.28% (21/171) and 24.56% (42/171) were accounted in the dry and wet seasons, respectively. Infection in G. pallidipes was significantly higher during the wet season (OR = 2.32; p < 0.05) and in park grazing areas (OR = 2.45; p < 0.05). In conclusion, trypanosomosis is the major challenge for cattle productivity in the district. So this study warrants the need for strengthening the vector and parasite control interventions in the area.

Bovine Mastitis: Prevalence, Risk Factors, and Bacterial Pathogens Isolated in Lactating Cows in Gamo Zone, Southern Ethiopia.

PURPOSE: Mastitis is a disease known to cause a great deal of loss of production and has a major economic impact. In the study area, there is little current information on bovine mastitis. Therefore, this study aimed to determine the overall prevalence of bovine mastitis and its associated risk factors and isolate the major pathogenic bacteria. METHODS: A cross-sectional study was conducted from February 2020 to September 2020 in selected dairy farms of Gamo Zone, southern Ethiopia. A total of 422 lactating cows were diagnosed for mastitis using the California mastitis test, clinical examination, and bacteriological methods. RESULTS: The overall prevalence of bovine mastitis determined in the area was 17.1% (72 of 422), of which 1.9% (eight of 422) was clinical and 15.2% (64 of 422) subclinical. Of 1,662 quarters examined, 7.94% (132) were positive. Bacteriological methods were also used to isolate the major pathogenic bacterial species associated with bovine mastitis. From 72 composite milk samples, growth of six different groups of bacteria was recorded in 64 (88.9%) samples. The most predominant bacterial pathogens isolated were Staphylococcus aureus (42.6%), ahead of Streptococcus spp. (26.2%), non-aureus staphylococci (14.8%), and Escherichia coli (11.5%). Salmonella spp. (3.3%) and Klebsiella pneumoniae (1.6%) were the least isolated bacterial pathogens. Among risk factors, breed, parity, udder depth, and tick infestation of the udder showed statistically significant differences (P<0.05) regarding the occurrence of mastitis. CONCLUSION: The current study revealed that mastitis is one of the health problems affecting dairy cows in Gamo. Enhancing the awareness of dairy farmers, regular screening, and improving hygienic conditions are critically important to control and prevent bovine mastitis in the study area.

Sero-prevalence of Newcastle disease and associated risk factors in chickens at backyard chicken production Kindo Koisha, Wolaita zone, Southern Ethiopia.

Newcastle disease (ND) is a serious infectious disease of poultry caused by virulent strains of Avian Paramyxovirus-1 and has a substantial impact on villages where people's livelihood depends upon poultry farming in several developing countries including Ethiopia. In the district of the study area, no previous studies have been conducted. Thus, the aim of the present study was to estimate individual and household flock level seroprevalence and risk factors for ND in unvaccinated backyard chickens in Kindo Koisha district, Wolaita zone, Ethiopia. A cross-sectional study design was conducted. For the study, household flocks were sampled as a cluster, and backyard chickens within the cluster with an age of more than 3 months were sampled. A total of 598 blood samples were collected from 86 household flocks during the study period. Serum samples were tested for ND antibodies using an Indirect-Enzyme Linked Immuno Sorbent Assay. The overall seroprevalence of ND virus at individual and flock level was 17.06% (95% CI: 14.25-20.29%) and 73.26% (95% CI: 62.79-81.64%), respectively. The ND seropositivity and associated risk factors were assessed at the individual bird and flock level by using ordinary and mixed effect logistic regression, respectively. Ordinary logistic regression revealed that crossbreed chickens had significantly higher odds of ND seropositivity than local breeds, with an odds ratio of 2.15 (95% CI: 1.54-3.00; p < 0.001). The odds of ND seropositivity was significantly higher in backyard chickens which belongs flock size >9 in comparison to <9 with an odds ratio of 3.7 (95% CI: 1.12-12.30; p < 0.031). The potential flock level risk factors related to ND seropositivity in this study were flock size, chicken house cleaning frequency, water source for chickens, dead chicken disposal practice, and distance to the next neighbor household, mixing with wild birds and owning pets. In conclusion, the current study generates significant information on the seroprevalence and the potential risk factors associated with ND at individual and flock level in Kindo Koisha district, Wolaita zone. Consequently, ND vaccination campaigns should be launched, and effective extension programs should also be provided to raise awareness about the disease.

Prevalence of Calf Mortality in Ethiopia: A Systematic Review and Meta-Analysis.

BACKGROUND: Calf mortality has been a major cause of economic losses in the dairy industry in Ethiopia. The condition results in a significant drop in the number of replacing heifers and bulls for sustainable dairy business. Reports on calf mortality with a wide range of prevalence are rising in the country; however, a pooled prevalence of this condition has not been established. Thus, this systematic review and meta-analysis aimed to quantitatively estimate the pooled prevalence of calf mortality in Ethiopia. METHODS: Meta-analysis was carried out to obtain the pooled prevalence of calf mortality in Ethiopia. A comprehensive literature search was carried out on PubMed, African Journals Online, CAB, Web of Science Direct, and Google Scholar. Eligible studies were selected based on predefined inclusion and exclusion criteria. Moderators such as the study area, breed of calf, study design, agroecology, and year of study article published were used as a milestone of data extraction. The random-effect model was used to estimate pooled prevalence. Publication bias and the variation in prevalence estimates attributed to heterogeneity were also assessed. RESULTS: Twenty-five original research papers on the prevalence of calf mortality in various parts of Ethiopia were included. The reported prevalence of calf mortality was between 0.9% and 37%. The pooled prevalence of calf mortality in the country was 14.79%, and the pooled calf mortality estimate across studies for the entire period regarding 1991 to 2000, 2001 to 2010, 2011 to 2016, and 2017 to 2020 was 26.54%, 17.03%, 14.21%, and 11.23%, respectively. Analysis of study subgroups and location revealed significant variations in prevalence. High heterogeneity was observed in the pooled estimates and even after the subgroup meta-analysis. The funnel plots and Egger's regression asymmetry coefficient (b = -1.0434) (95% CI = -1.49, -0.59; p value of 0.012) did suggest the presence of publication bias. There was also an indication of missing studies that could be incorporated by Duval and Tweedie's trim and fill method where they might fall on a funnel plot and visualize them in an attempt to increase the plot's symmetry. Analyses also suggest that calf breed, sample size, and study location are likely to be moderators of calf mortality prevalence in Ethiopia. CONCLUSION: This finding shows that calf mortality is widespread and could result in considerable economic losses for the dairy industry in Ethiopia. Inevitably, a significant reduction in calf mortality prevalence has been observed in recent years since 2010, but the reduction has not yet reached an economically tolerable level. Calf breed susceptibility contributed to the high prevalence. Therefore, interventions for increasing calf health and performance should be focused on minimizing calf mortality on farm and animal levels.

Longitudinal study of calf morbidity and mortality on smallholder farms in southern Ethiopia.

Calf morbidity and mortality are serious constraints in the success of dairy calf production. Thus, the current study was carried out with the objective to estimate the incidence of calf morbidity and mortality and associated risk factors in milk-shed districts of Gamo Zone, Southern Ethiopia. A prospective cohort and cross-sectional survey were employed from November 2019 to April 2020. A total of 196 calves were recruited by simple random sampling. Recruitment of calves was deployed by both the concurrent and prospective cohorts in calves aged below three months in study herds. The crude incidence of calf morbidity and mortality was 30.9% and 8.64%, respectively. The most frequently encountered disorder was calf diarrhea (10.17%), followed by pneumonia (6.5%). The other disorders were septicemia, omphalitis, arthritis, eye problem and miscellaneous cases. Multivariable Cox regression was revealed significant association for the calf vigor status, colostrum ingestion time, colostrum feeding status, dam parity, age at first calving, and related disorders were found risk factors of calf morbidity; likewise, calf vigor status at birth, time of colostrum ingestion and weaning were risk factors determining calf mortality. Calf morbidity and mortality rates recorded in this study were marginally higher than economically tolerable level, therefore, could affect the productivity of smallholder dairying by decreasing the obtainability of replacement heifers. Among significant explanatory factors investigated, colostrum ingestion time, method and amount were found important determinant factors of calf mortality and morbidity under the small-holder farming in the milk-shed districts of the Gamo zone. Therefore, rigorous calf husbandry practice is a need to manipulate the aforementioned calf determinants with subsequent application of tailor-made interventions.