Chemical composition, in vitro digestibility, and storability of selected agro-industrial by-products: Alternative ruminant feed ingredients in Israel.

The global demand for animal-based products is rising in the face of dwindling feed resources, and yet a huge pool of agro-industrial by-products (AIBPs) are generated, underutilized, and improperly deposited to landfills leading to environmental pollution. Ruminants have a special microbiome that can bioprocess and convert human inedible fibrous material into meat and milk, which appears as a great opportunity to simultaneously reduce pollution while promoting food security. In this study, we collected 15 domestically produced AIBPs from various regions of Israel during both winter and summer seasons to examine their potential as ruminant feed alternatives. We evaluated their storability, nutritional composition, and in vitro digestibility and performed a hierarchical cluster analysis to categorize them based on their distinctive nutritional characteristics. Among the 15 AIBPs, 8 have rich essential nutrients, and minerals, and have excellent in vitro digestibility, but they have less than 6 days of storability and develop off-odours. Out of 15 AIBPs; 8 have low dry matter (DM) content, ranging from 4.7% to 30.45% while the remaining 7 AIBPs have high DM, ranging from 50.6% to 98.6%. The high crude protein (CP) category included 6 AIBPs with CP ranging from 19.7% in beer pulp to 32.1% in jojoba cake. Starch content was high in 3 AIBPs ranging from 33.7% in timorim mix to 65.2% in Irish potato culls. Considerable crude fat content was reported in 4 AIBPs, the highest being yoghurt waste with 42.8%. In terms of neutral detergent fiber (NDF), 5 AIBPs had low NDF content ranging from 0% to 14.1%; 5 AIBPs had moderate concentration ranging from 34.3% to 50.7%, and 5 AIBPs had high levels between 66.6% and 82.8%. Interestingly, 10/15 AIBPs had medium to high in vitro dry matter digestibility (IVDMD). This study, therefore, suggests that recycling AIPBs for livestock nutrition has enormous potential that is still underutilized and offers excellent ways to gain socioeconomic and environmental benefits by expanding animal feed resources and reducing feed-food competition, and landfill burden. However, additional studies are necessary to focus on affordable storage technology to prolong the storability of AIBPs and feeding trials to determine the productive performance of livestock fed an AIBPs-based diet.

Reducing dietary sodium of dairy cows fed a low-roughages diet affect intake and feed efficiency, but not yield.

Wastewater from dairy farms has become a major environmental and economical concern. Sodium residue in treated and untreated wastewater from dairy farms used for irrigation can lead to soil and groundwater salinization, with the risk of soil degradation. We examined the effect of reducing sodium fed to mid to late lactating cows from 0.61% (high sodium [HS]) to 0.45% (low sodium [LS]) of dry matter on dry matter intake (DMI), milk and milk-component yields, eating behavior, apparent total track digestibility, feed efficiency, and sodium excretion into the environment. We randomly assigned 28 multiparous high-yielding ( > 35 kg milk/d) cows to 1 of 2 treatment groups (LS or HS) in a crossover design, with 7 d of adaptation and 28 d of data collection. Reducing sodium in the diet reduced sodium intake from 171 to 123 g/d while lowering sodium excreted in the manure by 22%. Energy corrected milk (ECM) yield (37.4 kg/d) and sodium excretion in the milk (33.7 g/d) were similar for both groups. The DMI of LS cows was lower than that of HS cows (27.3 vs. 28 kg/d) and consequently, feed efficiency of the LS cows was higher (1.40 vs. 1.35 ECM/DMI). Eating rate, meal and visit frequency, and eating time were similar for both treatments; meal and visit duration were longer for the HS cows, and meal and visit sizes tended to be larger. Digestibility of DM and amylase-treated neutral detergent fiber remained similar. Based on the results of this study, and discussed considerations, we recommend lowering the dietary sodium content for mid to late lactating cows in commercial herds to 0.52% of DM, in order to reduce sodium excretion to the environment via urine.

Microbiome niche modification drives diurnal rumen community assembly, overpowering individual variability and diet effects.

Niche modification is a process whereby the activity of organisms modifies their local environment creating new niches for other organisms. This process can have a substantial role in community assembly of gut microbial ecosystems due to their vast and complex metabolic activities. We studied the postprandial diurnal community oscillatory patterns of the rumen microbiome and showed that metabolites produced by the rumen microbiome condition its environment and lead to dramatic diurnal changes in community composition and function. After feeding, microbiome composition undergoes considerable change in its phylogenetic breadth manifested as a significant 3-5-fold change in the relative abundance of methanogenic archaea and main bacterial taxa such as Prevotella, in a manner that was independent of individual host variation and diet. These changes in community composition were accompanied by changes in pH and methane partial pressure, suggesting a strong functional connection. Notably, cross-incubation experiments combining metabolites and organisms from different diurnal time points showed that the metabolites released by microbes are sufficient to reproduce changes in community function comparable to those observed in vivo. These findings highlight microbiome niche modification as a deterministic process that drives diurnal community assembly via environmental filtering.

Effect of feeding ensiled mixture of pomegranate pulp and drier feeds on digestibility and milk performance in dairy cows.

Based on a previous ensiling study in glass silos of various pomegranate pulp (PP) mixtures, fresh pomegranate pulp (PP) was mixed with drier feeds including soy hulls and corn silage (40:35:25 on DM basis) and ensiled in 32 pressed bales (700 kg each) wrapped with stretch polyethylene film. This ensiled pomegranate pulp mixture (PPM) was included in lactating cow total mixed ration (TMR) at a level of 20% of DM (PPM-TMR). Performance and digestion experiment was conducted with two groups of 21 milking cows each, fed individually one of the two TMR: 1. Control TMR without ensiled PPM; 2. Experimental TMR which contained 20% ensiled PPM, including 8% PP as corn grain replacer. Voluntary DM intake of cows fed the control TMR was 5.04% higher than that of the PPM cows. In vivo digestibility of DM, OM, NDF, CP and fat were significantly higher in the control cows compared with the PPM group, but methane production in the rumen fluid was 25% lower in the PPM cows. A slightly higher milk yield (by 2.2%) observed in the control cows; however, milk fat content was 5.9% higher in the PPM cows. This was reflected in similar yield of energy corrected milk (ECM) and 3.97% increase in production efficiency (ECM/DM intake) of the PPM cows compared with the control ones. Welfare of the cows, as assessed by length of daily recumbence time, was in the normal range for both groups. Body weight gain was also similar in both groups. Data suggest that the level of 8% PP in the PPM-TMR used in this study was probably too high for lactating cows and should be lowered to 4% in order to achieve better performance.