Effect of Platinum Nanoparticles on the Structure of Protein Molecules and Regulation of the Tone of Brain Vessels in Experimental Animals.

The effects of platinum nanoparticles on the morphological structures of the solid phase of blood serum and the tone of cerebral microvessels as indicators of the dynamics of homeostasis were studied on outbred albino male rats (n=40) weighing 300-350 g. Platinum nanoparticles were injected to experimental animals in 1 ml of physiological saline. For systemic BP measurements and blood sampling, the femoral artery was isolated and catheterized. The study of solid phase structures of blood serum was conducted by the method of cuneiform dehydration. The results showed that injection of platinum nanoparticles significantly affected the body of experimental animals.

A Marker of Cerebral Ischemia in Solid State Structures of Blood Serum.

Reversible cerebral ischemia of medium severity was reproduced in male Wistar rats by bilateral occlusion of the common carotid arteries. Solid-phase structures (anisomorphons) were obtained by marginal dehydration of the serum. Small focal isotropic defects in the serum anisomorphon texture were found in 100% cases during occlusion of the carotid arteries. Similar signs were detected in all patients with chronic cerebral ischemia, which proved specificity of this morphological marker of the disease.

Blood serum structures integrated assessment of pathophysiological changes in experimental cerebral ischemia.

The purpose: The purpose. To reveal solid-phase structures of blood serum during and after experimental cerebral ischemia. Methods: Integrated estimation pathophysiological changes at brain ischemia received on solid-phase structures of blood serum (BS) at 27 rats-male Vistar in weight of 300-390 g. Reversible brain ischemia of moderate severity was reproduced imposing surgical clips on both common carotids for 15 minutes then clips removed and provided bloodstream restoration on carotids. Blood took away in Eppendorf`s test tube in volume on 1 ml before imposing clips, directly ahead of their removal and in 30 minutes after bloodstream restoration. By the Method cuneiform dehydration received BS facia (dry a film) which structures analyzed by means of stereomicroscope MZ12 firm «Leica¼. Results of researches fixed on structural parameters BS before imposing clips, after removal clips and in 30 minutes after bloodstream restoration. Results: Before ischemia the integrated picture facias BS rats had the harmonious system organisation: the radial or partially-radial arrangement of the cracks, accurately generated konkrecias that testified to normal physiological condition of experimental animals. During the period occlusions carotids there were markers of a ischemia, activation of a capillary blood-groove, inflammatory reaction and stress. Partially these signs remained at reperfusion. Conclusion: The conducted researches of structures BS on experimental animals have allowed to reveal marker structures BS similar revealed at inspection of people with an ischemia of a brain.

[Features of Urine Biomineralization in Long-Livers].

BACKGROUND: The increasing number of long-livers in the developed countries indicates the demand for clarification of the body vital systems' functioningfeatures which allow the long-livers to prolong their active involvement in social life. OBJECTIVE: Aim of our study/research was to define a functional condition of urinary system in long-livers using the new diagnostic technology which analyses the structures of biological liquid at its phase transition in a solid state. METHODS: A case series study was held. All long-livers suffered from atherosclerosis and its complications as well as from associated diseases. An exclusion criterion was acute conditions. The creatinine level in blood serum, indicators of common urine analysis and features of urine facies (using cuneiform dehydration) were defined. RESULTS: Participants included 60 long-livers (mean age 87.34±4.17 years) who were passing clinical laboratory tests at in-patient department of gerontological hospital. In urine facies of 41 (68.3%) long-livers a phenomenon of salt crystals distribution was identified. It presents the concentration of anisotropic salt crystals in the form of a ring in a regional facies zone. The tenfold increase in concentration of calcium and double increase in concentration of phosphorus in comparison with the central zone was detected in long-livers with a higher percent sulphur in the regional zone of urinefacies. CONCLUSION: The urine facies of the majority of long-livers have signs of age structure formation associated with a specific distribution of anisotropic salt crystals which fix detritus in its inert form to block the autointoxication.

[Morphological marker of tumor progression in laryngeal cancer].

Dynamic observation of 32 patients with laryngeal cancer, treated with radiotherapy and combination therapy, was performed using diagnostic technology "Litos-system", including the definition of serum morphotypes by marginal dehydration of biological fluids. In nine of them, on the background of the treatment, there was continued growth of the tumor. In the blood serum seven of nine patients had special microspheroliths, characterized by the presence of "wavy" structures. Such "wavy" microspheroliths could be seen as a harbinger of the continued growth of the tumor, because after their appearance there were detected signs of clinical progression. Apparently, the appearance of mentioned "wavy" structures in the patient's blood is a result of release into the blood of qualitatively new macromolecules produced by a clone of malignant tumor cells.

[Current views of etiopathogenetic mechanisms underlying the development of chronic pyelonephritis in subjects of different age].

A literature review of the issues pertinent to etiology and pathogenesis of chronic pyelonephritis is presented. Different aspects of pathogenesis of the disease in selected age groups of the patients (from childhood to senility) are considered and analysed with special reference to the etiology of acute and chronic pyelonephritis as well as the risk factors of this disease.

[Peculiarities of diagnostics of weakly symptomatic clinical course of chronic pyelonephritis].

The difficulty of early diagnostics of weakly symptomatic clinical course of chronic pyelonephritis (CP) is attributable to the lack of adequate methods. We used a novel Litos system diagnostic technology based on morphological analysis of dehydrated biological fluid The aim of the study was to develop methods for CP diagnostics from morphological characteristics of urine at different stages of the disease. It included 108 patients aged 58+/-1.3 yr with CP and 24 practically healthy subjects. Standard examination of all patients was supplemented by the analysis of urine using the Litos system. It was shown that morphological picture of urine makes it possible to objectively detect not only complete remission or exacerbation of the disease but also partial remission and high risk of CH even in patients with weakly symptomatic clinical course of this pathology. In case of incomplete remission, the treatment requires adequate long-term (three-course) antibacterial therapy despite normal results of urine analysis and microbiological studies. Patients at high risk of CP need to be prescribed preventive phytotherapy.

[Medical and social characteristics of the very old Moscow citizens and World War II veterans].

The very old people is a growing population group in Russia and in Moscow in particular that requires more close attention as it demands special medical and social approaches. The investigation was based on data obtained from Moscow district clinics concerning the gender and pathologies structure of people aged 90 yrs and older. In this group women prevailed in total amount and among those older 100 yrs. Striking gender difference was revealed in disturbances of the carbohydrate metabolism. The most frequent pathologies in this population are cardiovascular, nerve and gastrointestine duct systems diseases that have equal prevalence among men and women. Centenarians appeared to be healthier than those in the group of 90-94 yrs. Many of them could serve themselves without extra help (36,48% versus 13,06%). The results obtained in this research must be helpful in the organization of geriatric clinics and cabinets.

[The marker of discogenic diseases of the nervous system in the cerebrospinal fluid].

Using a novel diagnostic technology that allows to investigate the structure of a biological fluid formed during its phase transition into a dried film, we revealed a cause of mistaken results of protein concentrations in the cerebrospinal fluid of patients with discogenic radiculitis. The traditional laboratory study does not reveal the elevated content of protein in patients with discogenic radiculitis and hernia of invertebral discs due to its more active binding with salts with the following sedimentation during centrifugation. It can be explained by the involvement of salt crystals in the formation of the inert organic-mineral aggregate with protein molecules which structure was changed by dystrophy, ischemia, hypoxia, mechanic damage, tumor process. The aggregate is characterized by abnormally tight links. This phenomenon is known as biomineralization, the universal mechanism preventing the organism from toxic effects of products of degraded tissues.

[Organization of the geriatric service in conditions of progressing demographic aging the population of the Russian Federation].

In this article the critical analysis of a state of geriatric service in the Russian Federation is given. It is marked, that actually geriatric service is submitted by a wide network of establishments of various functional orientation and specialization: the establishments rendering the medico-social help to elderly patients in-home, in outpatient and stationary conditions. Thus territorial distribution of establishments is of non-uniform and not always corresponds to the actual requirements of region. The geriatric service has not quite satisfactory legal base. It has no vertical administrative connections, and horizontal connections carry mainly initiative, fragmentation forms. Staff of service has no standardization; permanent appointments of geriatric doctors are not entered in list of staff of treatment-and-prophylactic establishments. The professional training of geriatric doctors is carried out only in some educational institutions, and experts of the average level are actually not trained anywhere. All these lacks create the certain pressure in medico-social service of the senior age population and do not enable to use actual potential of geriatric service in full. Some variants on a possible exit from the situation are offered in the article.

Diagnostic markers in the structures of human biological liquids.

INTRODUCTION: To date, morphology research in laboratory diagnostics has referred only to cell tissues and excluded biological fluids. Our new technology is based on the study of structures of biological fluids formed during transition from liquid to solid state by dehydration under special conditions. These structures are formed due to the special "mosaic" configuration of molecules and microaggregates of the substance (protein and others) dissolved in the studied fluids. It should be considered as well that the specific structure of protein molecules is a fundamental basis of anatomical and physiological peculiarities of an organism. All pathological processes are initiated by the changes in the spatial configuration of protein molecules. METHODS: We have developed a special method named "cuneiform dehydration of biological liquids" to obtain thin films which possess specific structures. During the transition of a biological liquid into a solid, organic molecules build specific macrostructures in conformity with their configuration. RESULTS: Studies of the solid phase structures of different biological liquids allow us to find specific markers of various pathological processes in the human organism. In blood serum, we discovered markers of inflammation, sclerosis, chronic intoxication; in tear--markers of different stages of glaucoma; and in synovial fluid--markers of different stages of osteoarthrosis. In our investigation of 143 healthy people and 1,419 patients, a high rate of compliance of the discovered markers with the results of routine clinical and laboratory methods were observed. As the suggested technology has a higher sensitivity, the results obtained by this technology and routine methods do not always coincide. CONCLUSION: The developed technology gives new information about the state of the human organism and may be used in practice for diagnostics of various pathological processes and at pre-clinical studies of their development, as well as for the evaluation of the efficacy of medical treatment.

[Morphological structure of oral liquid--diagnostic possibilities].

This research has revealed the morphological structure of the oral liquid obtained from 187 adolescents on the basis of self-organized process occurring during the phase transition "liquid-solid". The results of the research have been compared with the data obtained in the course of dental checkup. The parameters of the oral liquid morphological structure in case of healthy periodontium have been identified and the markers of pathology caused by periodontal tissue inflammation and caries have been determined.

[Morphometric analysis of the structure of tear and aqueous humor in senile cataract].

The parameters of the wedge dehydrated samples of aqueous humor and tear taken from patients with senile cataract were compared by a morphometric assay. Computer-aided processing of the images of the test biological fluids was made on a Morphotest-1 hardware-software complex. The study has provided evidence for the uniformity of distribution of major structural components in the dehydrated samples of tear and aqueous humor in each specific examinee. This fact opens up possibilities for studying the signs of different intraocular pathological conditions from the morphological pattern of tear.

Bio-liquid morphological analysis.

Information is presented on the new scientific line in medicine and biology: bio-liquid morphology. The interdisciplinary character of the given research area is emphasized. The problems and prospects of bio-liquid morphological analysis development both in applied and fundamental aspects are discussed.

[A morphological pattern of the aqueous humor in the evaluation of pathophysiological mechanisms of involutional cataract genesis]. / Morfologicheskaia kartina vodianistoi vlagi v otsenke patofiziologicheskikh mekhanizmov involiutivnogo kataraktogeneza.

The method of wedge-type dehydration was made use of to study the specificity of the systemic structure of 496 samples of aqueous humor obtained in the course of surgical interventions in 62 patients for senile cataract. The results were analyzed with respect to a cataract stage and it was established that the systemic structure of aqueous humor reflects different-phase impairments in the system of the intermolecular interaction of aqueous-humor components, which is mainly related with activated processes of organic products' mineralization as observed in senile cataract progression.

[Fundamental bases of biological rhythms]. / Fundamental'nye osnovy biologicheskikh ritmov.

The data and theoretical points given in the paper mould basically new views of molecular relationships underlying the function of living beings and biological rhythms. The authors' procedure for wedge biological fluid dehydration reveals a wide autowave spectrum that is clearly detectable when the fluid passes into the solid phase. A hypothesis of the autowave interaction of biologically active molecules is forwarded, which considers autowaves as a basis of organization of physiological and pathological processes occurring in the body.

[The methodological characteristics of determining the basic and trace elements in the serum and peripheral blood cells by the x-ray fluorescence method]. / Metodicheskie osobennosti opredeleniia osnovnykh i mikroélementov v syvorotke i kletkakh perifericheskoi krovi rentgenofliuorestsentnym metodom.

Analytical parameters of instrumental methods of the element analysis of biomedical objects are compared and the place occupied by x-ray fluorescent analysis (RFA) among them is demonstrated. The methods for preparing the specimens of liquid media of the organism and the RFA are simple, rapid, involve no disintegration of the sample, and allow measurements of elements at sigma 1 = 0.02 at concentrations of 12 micrograms/ml. The method is preferable and promising for some basic elements (P, S, Cl, K, Ca), which are difficult to measure by other methods. The levels of S, K, Ca, Fe, Zn, and Cu were measured in erythrocytic mass and blood serum of normal subjects, patients with asthma and aplastic anemia, and recipients of allogenic kidneys.