RESUMO
The effects of varying the experimental conditions of the preparation of the renal scanning agent 99mTc-PMT on its organ distribution have been investigated. The stability of 99mTc-PMT was determined in vitro. A procedure for the preparation of this agent is recommended.
Assuntos
Compostos Organometálicos/síntese química , Compostos de Organotecnécio , Pirrolidinas/síntese química , Kit de Reagentes para Diagnóstico , Tecnécio , Tetraciclinas , Animais , Rim/diagnóstico por imagem , Compostos Organometálicos/metabolismo , Pirrolidinas/metabolismo , Coelhos , Cintilografia , Ratos , Ratos Endogâmicos , Tetraciclina/síntese química , Tetraciclina/metabolismo , Distribuição TecidualRESUMO
Using gel chromatography two different 99mTc-PPi complexes (complex I and II) were observed under various pH-values. In neutral medium complex II was the main product while in acidic or alkaline media complexes I and II were formed. Our results related to the organ distribution in mice revealed that complex II is a bone-seeking agent whereas complex I concentrates in the kidneys.
Assuntos
Difosfatos , Tecnécio , Animais , Osso e Ossos/diagnóstico por imagem , Cromatografia em Gel , Difosfatos/metabolismo , Concentração de Íons de Hidrogênio , Rim/diagnóstico por imagem , Camundongos , Coelhos , Cintilografia , Tecnécio/metabolismo , Pirofosfato de Tecnécio Tc 99m , Distribuição TecidualRESUMO
A one-step freeze-dried Sn-glucose kit was developed to be labelled with technetium-99m. TLC, paper chromatography and gel chromatography column scanning techniques (GCS) were used for the determination of the labelling yield of the complex. High radiochemical purity was obtained (98%). In vivo distribution studies were performed in white rats. Maximum uptake in kidneys (28%) was achieved in 15-90 min. The blood disappearance curve studies of the labelled Sn(II)-gluco-ene-diolate in rabbits have shown two disappearance phases of activity from blood with biological half-times of 10 and 150 min for the fast and slow component respectively.
