[Indicators of lipid metabolism and the blood lipid peroxidation system in men with regard to hereditary predisposition to atherosclerotic vascular pathology]. / Pokazateli lipidnogo obmena i sistemy perekisnogo okisleniia lipidov krovi u muzhchin s uchetom nasledstvennoi predraspolozhennosti k ateroskleroticheskoi sosudistoi patologii.

Lipid metabolism and the blood lipid peroxidation system were examined in 56 military males living in rather similar conditions. The parameters in question were compared in the following groups: (1) control subjects, including healthy individuals without a family history of atherosclerotic vascular abnormalities; (2) healthy subjects with a family history of atherosclerosis; (3) patients with coronary heart diseases. There were significant differences only in single cases between the groups. The application of a system of grids setting upright the distribution curves for the parameters under study proved to be effective in finding significant differences between the groups, showing the value of the hereditary factors in the development of atherogenic lipid changes.

[Indicators of lipid peroxidation in the blood in hereditary predisposition to arteriosclerosis]. / Pokazateli perekisnogo okisleniia lipidov krovi pri nasledstvennom predraspolozhenii k aterosklerozu.

In members of the families whose parents had atherosclerosis complicated by macrofocal myocardial infarction or stroke, the serum level of lipid peroxidation products was correlated to enzymatic activity of neutrophil and red blood cells oxidation-antioxidation. In persons with hereditary predisposition to atherosclerosis both with clinical signs of atherosclerosis and phenotypically healthy against the control group there was elevated content of plasma acylhydroperoxides and hypoactivity of neutrophil myeloperoxidase. Determination of lipid peroxidation products by malonic dealdehyde showed this parameter to be higher in members of the families of the study group and in those with cardiovascular disorders. For those whose parents had atherosclerosis versus control subjects there were no differences in the activity of superoxide dismutase, glutation peroxidase and catalase in the blood red cells. Shifts in lipid peroxidation and activity of blood myeloperoxidase are identical in type and may represent a pathogenetic ling in formation of hereditary predisposition to cardiovascular disorders of atherosclerotic origin, the detection of which becomes feasible in a subclinical period.

[Blood lipids and lipid peroxides in the families of patients with ischemic heart disease]. / Lipidy i lipoperoksidy krovi v se'miakh bo'lnykh ishemicheskoi bolez'niu serdtsa.

Levels of some fractions of blood neutral lipids and lipoperoxides were compared in the families of patients with coronary heart disease. The patients and their relatives (including healthy ones) were found to show a statistically significant increase in the atherogenic index, total cholesterol, low density lipoprotein cholesterol, triglyceride, total lipid, and lipoperoxide concentrations and a decrease in high density lipoprotein cholesterol levels as against controls. The changes in the values of lipids and lipoperoxides in the families of patients with coronary heart disease were homogenous and might be one of the pathogenetic links in the formation of hereditary predisposition to atherosclerotic cardiovascular diseases which might be detected in a prehospital period.

[Atherosclerosis and the process of lipid peroxidation]. / Ateroskleroz i protsess perekisnogo okisleniia lipidov.

Reported data on lipid peroxidation (LPO) in atherosclerotic cardiovascular diseases are summarized. Numerous evidences are presented in favor of the fact that atherosclerotic vascular lesions are accompanied with disordered blood LPO, resulting in the accumulation of excessive quantities of peroxide derivatives, an important pathogenetic sign of this disease, along with blood lipid and lipoprotein levels, and clinico-instrumental findings. Oxidation and antioxidation factors whose balanced interaction contributes to the control of LPO activity are described.

[Serotonin concentration and transport in the blood platelets of ischemic stroke patients and their relatives]. / O soderzhanii serotonina i ego transporte v trombotsitakh krovi bol'nykh s ishemicheskim insul'tom i ikh rodstvennikov.

Serotonin levels and transport were studied in patients with ischemic stroke (n = 31), their relatives (n = 50) and clinically healthy subjects (n = 45). A statistically significant difference was found in the absorption of exogenic serotonin in the patients and relatives. It has been shown that if judged by serotonin levels, the distribution curves in the control subjects patients with ischemic stroke and their relatives correspond to a model of the polygenic type of the heredity of the traits studied.

[Disorders in the lipid peroxidation system of the blood neutrophils of patients with the Shereshevskii-Turner syndrome]. / Narusheniia v sisteme perekisnogo okisleniia lipidov neitrofilov krovi u bol'nykh s sindromom Shereshevskogo-Ternera.

The neutrophil leukocyte test with the use of the antioxidant adrenaline was applied to study lipid peroxidation (LPO) in patients with Shereshevsky-Turner's syndrome. The data obtained point to the activation of LPO and reduction of the activity of the antioxidant system in neutrophil leukocytes.

[Lipid peroxidation and the myeloperoxidase activity of neutrophilic leukocytes in shereshevskyi-Turner syndrome]. / Perekisnoe Okislenie lipidov i aktivnost' mieloperoksidazy neitrofil'nykh leikotsitov pri sindrome Shereshevskogo-Ternera.

The level of lipid peroxides, activity and thermostability of myeloperoxidase have been studied in neutrophil extracts from the peripheral blood of patients with the Shereshevsky-Turner syndrome and in normal subjects. A statistically significant rise in the level of lipid peroxides and respective decrease in the activity and thermostability of myeloperoxidase have been revealed in the patients as compared with the control group. These findings point to functional inferiority of neutrophils. It is probable that at the basis of the disorders seen there lie an abnormal set of sex chromosomes and upset hormonal regulation determined by the abnormality.

[Submicroscopic and immunochemical study of horseradish peroxidase passage through the epithelium of the bronchial mucosa]. / Submikroskopicheskoe i immunokhimicheskoe izuchenie prokhozhdeniia peroksidazy khrena cherez épitelii slizistoi bronkha.

2% solution of horseradish peroxidase (HRP) was placed on the bronchial mucosa of anaesthetized dogs through a bronchoscope. Arterium femoralis and thracal lymphatic duct were canulated to HRP application. The radiate immunoprecipitation method was utilized to assess HRP activity in blood and lymph samples. In blood, a progressive increase in HRP activity was noted during the experiment, while this activity in lymph rose in 30 minutes, to decrease by the end of the experiment. In parallel series of experiments biopsy specimens were taken from bronchial mucosae exposed to HRP solution. Within all the intervals after HRP administration tested, the product of reaction was clearly demonstrated ultrastructurally within the intracellular spaces and under basal lamina of bronchial epithelium. Pictures suggestive of endocytosis were infrequently encountered in the apical portion of ciliated cells.

[Immunochemical study of the leukocyte myeloperoxidases from various sources]. / K immunokhimicheskomu izucheniiu mieloperoksidaz leikotsitov iz raznykh istochnikov.

The antigenic difference between myeloperoxidases of human, rabbit, guinea pig, horse, dog, sheep and mouse leucocytes and horse radish peroxidase was investigated. By counterimmunoelectrophoresis with antiserum specific for human and mouse myeloperoxidase and horse radish peroxidase, the enzyme catalysing peroxidase reaction in leucocytes from the above sources was shown to possess species specificity and different antigenic composition.

[Isolation and properties of myeloperoxidase from human bone marrow]. / Vydelenie i nekotorye svoistva mieloperoksidazy kostnogo mozga cheloveka.

A preparation of myeloperoxidase was isolated from human bone marrow by column chromatography on DEAE-Sephadex, CM cellulose and gel filtration on Sephadex G-100. The enzyme was purified to 0.76 purity with a 16% yield. The repeated gel filtration, E430/E280 ratio and immunochemical study confirm the high degree of purification. Molecular weight of the enzyme, determined by gel filtration on Sephadex G-100, was 150000.

[Immunochemical characteristics of the peroxidases of several mouse tissues]. / Ob immunokhimicheskoi kharakteristike peroksidaz nekotorykh tkanei myshei.

Enzymes catalyzing peroxidase reaction of a lysosomal fraction in bone marrow, leucocytes, spleen, thyroid gland, stomach, kidney, heart, lungs, brain and skeletal muscle of mice were investigated by immunochemical methods. A high level of peroxidase activity was discovered in leucocytes, bone marrow, spleen, heart and lung, a lower activity appeared to be characteristic of liver, thyroid gland and kidney. The peroxidase activities in brain, skeletal muscle and stomach were low. The reaction of immunoprecipitation with myeloperoxidase-specific antiserum revealed considerable antigenic distinctions between the enzymes catalysing peroxidase reaction in various tissues of mice.