Correlation between the middle phalanx of the third finger skeletal maturation staging system and insulin-like growth factor-1 levels among Indian children - A 3-year longitudinal study.

INTRODUCTION: Tracking insulin-like growth factor-1 (IGF-1) level alongside the middle phalanx of the third finger (MP3) staging modification could provide valuable insights into the relationship between hormonal factors and skeletal maturation during different stages of growth. Longitudinal studies indeed play a crucial role in understanding these complex relationships over time, allowing for a more comprehensive assessment of how IGF-1 might serve as a marker for pubertal growth stages. METHODOLOGY: The present longitudinal prospective cohort study was done among 26 boys and 26 girls. For each child, once in every 6 months for 3 years, blood samples (to estimate IGF-1 level) and X-rays of the left hand middle finger were taken. DRG IGF-1 600 Human Enzyme-Linked Immunosorbent Assay kit was used for the quantitative measurement of IGF1 from serum. The MP3 stages of the middle phalanx of the middle finger were evaluated using a modified MP3 system. The collected data were subjected to suitable descriptive and inferential statistics. RESULTS: The mean IGF1 levels were significantly higher in girls compared to boys across all the stages of MP3 skeletal maturity indicators. However, in both boys and girls, IGF-1 levels showed increasing trends from Stage F to H where it peaked and showed deceleration to Stage I (P < 0.05). CONCLUSION: The observed increase in serum IGF-1 levels during pubertal stages, followed by a decline in late puberty, aligning with the stages of skeletal maturation, suggests a close relationship between hormonal changes and bone development.

Combined remineralizing effect of arginine and fluoride on artificially demineralized human primary dentin evaluated using quantitative light induced fluorescence.

Introduction: One the most common chronic dental diseases affecting children is dental caries. Dentin caries is a condition in which caries has progressed to the dentin and caused a significant depth of lesion. Clinical studies have revealed that an increased caries risk is associated with a decreased alkali-producing capacity of the microbial populations colonizing the oral cavity of adults, which arginine somewhat compensates for. Aims: To evaluate the remineralizing efficacy of fluoridated toothpaste, with fluoride-arginine containing toothpaste on demineralized dentin of primary teeth using quantitative light-induced fluorescence™. Materials and Methods: Forty-five primary molars were decoronated and sectioned to prepare dentin specimens and mounted in an acrylic block in a uniform manner using a customized acrylic jig. Samples were randomized into three groups, were subjected to demineralization to create artificial dentin caries lesion. Following this, all the 45 samples were subjected to multispecies bacterial pH cycling for 21 days. All the specimens were evaluated for postdemineralization, pH cycling day 7, 14, and 21 on QLF™. Results: On day 21, maximum fluorescence gain was observed by the positive control group followed by the arginine and negative control group. The variation observed between positive control and arginine group was found to be statistically significant. Conclusions: An in vitro development of artificial caries such as demineralized lesion on primary dentin sample using plaque biofilm was observed successfully under QLF after 72 h. Arginine in combination with fluoride showed almost similar remineralization of demineralized primary dentin compared to fluoride alone after 21 days of multispecies bacterial pH cycling.

Diet's Cariogenicity and Healthfulness Index (DCHI) - A Novel, Comprehensive Index for Children.

BACKGROUND: Realistic and tailored dietary advice plays a vital role in Preventive Dentistry. An appropriate diet analysis forms the basis for precise dietary advice. Analysis of diet for its cariogenicity is a complex process. Performing a meaningful diet analysis is a challenge in routine clinical practice. Currently, very few tools are available for clinicians to assess children's diet relating to caries risk. The current scientific paper presents a novel index that assesses the cariogenicity and healthfulness of a child's diet objectively and guides the professional to achieve pragmatic diet modification in the prevention of dental caries. METHODS: Multi-disciplinary teams with a sample study population were involved in the initial structuring of Diet's Cariogenicity and Healthfulness Index. To obtain cut-off values for cariogenicity of diet, a study was carried out with a sample of 774 children correlating diet diary with caries status. For statistics, the Receiver Operating Characteristic (ROC) curve closest to the ideal of 100% sensitivity and 100% specificity was applied. RESULTS: ROC curve of 22.5 were plotted for diet scores for its cariogenicity. The sensitivity, specificity, and area under the curve of these cut-off values were 70 and 95, respectively. CONCLUSIONS: The current scientific paper presents a novel Diet's Cariogenicity and Healthfulness Index that assesses the cariogenicity and healthfulness of a child's diet objectively to guide the professional to achieve a logical diet modification.

Quantification of tongue coating using quantitative light-induced fluorescence-digital image analysis and its correlation with visual evaluation.

BACKGROUND: The tongue coating is a grayish-white deposit that quickly reflects the state of health or disease of the human body. Quantitative light-induced fluorescence (QLF) is a novel digital imaging system that objectively quantifies tongue coating. AIMS: The present study aims to evaluate the correlation between the visual assessment of tongue coating and tongue coating by analysis of QLF-digital (QLF-D) images. SETTINGS AND DESIGN: This was an in vivo explorative study. MATERIALS AND METHODS: Fifty children aged 11-13 years with clinically visible tongue coating were selected for the study. Tongue coating was assessed clinically by the Tongue Coating Index (Shimizu et al., 2007) and digitally by QLF-D Biluminator™ 2, C3 software. STATISTICAL ANALYSIS: Data obtained were subjected to statistical analysis using SPSS 23.0 software. Spearman's rho correlation test was done, and P < 0.05 was considered statistically significant. RESULTS: A statistically significant correlation was found between the visual assessment scoring and the QLF image analysis for the evaluation of tongue coating. CONCLUSION: The Digital QLF tongue imaging system was found to be reliable due to its correlation with the clinical score and objective nature.

Comparative assessment of the efficacy of low concentration bleaching agents using quantitative light induced fluorescence in removing stains: An In vitro study.

Background: Tooth discoloration has become a common esthetic problem in recent years. Removal of stains by bleaching is well-documented. Low concentration home bleaching products are available in market in different forms and concentrations. Aim: The aim of this study is to evaluate and compare the efficacy of low concentration commercially available home bleaching products (whitening strip, gel, and mouthwash) in removing stains and whitening the tooth using clinical and digital methods. Materials and Methods: Sixty permanent enamel samples mounted in an acrylic block were artificially stained and randomly divided into four groups. Negative control, 15 % Carbamide peroxide gel group, 2% Hydrogen 16 peroxide mouthwash group and 6% Hydrogen peroxide strip group respectively. The samples were bleached with respective agents according to the manufacturer's instructions. The efficacy on 7th and 14th day was evaluated clinically (SGU change), photographically (ΔE), and using quantitative light-induced fluorescence (ΔF). The data were analyzed using paired t-test and analysis of variance. Results: Postbleaching, 6% hydrogen peroxide strips and 15% carbamide peroxide gel showed maximum improvement (ΔΔF - 15.73 and 11.89, ΔE - 19.8 and 18.9, respectively) when compared to 2% hydrogen peroxide mouthwash and negative control group (ΔΔF - 9.68 and 6.59, ΔE - 15.04 and 9.44, respectively). The difference was statistically significant (P = 0.001). Conclusion: 6% hydrogen peroxide strips and 15% carbamide peroxide gel showed maximum improvement in stain removal and tooth whitening however, the strips showed better efficacy than the gel. Strips have the added advantage of lesser contact period, less salivary dilution, and no gingival contact. Therefore, strips can be a better alternative for gels and mouthwashes.

An in vitro evaluation of ice apple water, Aloe vera, and propolis as a storage medium to preserve viability of human periodontal ligament fibroblasts.

Background: A number of media that create the best possible conditions to maintain periodontal ligament (PDL) cell viability after dental avulsion have been reported. Aim: The aim of this study is to evaluate ice apple water (IAW), Aloe vera, and propolis as a storage medium to preserve the viability of human PDL fibroblasts. Methods: An in vitro comparative type of study was performed on a PDL cell culture model. PDL fibroblasts obtained from the roots of healthy premolars were cultured in Dulbecco's Modified Eagle's Medium (DMEM) and treated with ice apple water (IAW), 7% propolis extract (PE), 30% Aloe vera extract (AVE), positive control DMEM supplemented with fetal bovine serum, negative control (NC) without any agent, and incubated at 37°C for 1 h, 3 h, and 24 h. Cell viability was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay after every test period. Optical density was measured at a wavelength of 490 nm. Statistical Analysis Used: The effects of the test storage media were evaluated by one-way analysis of variance test, followed by post hoc Tukey's multiple comparison test (P < 0.05). Results: Seven percent PE demonstrated the highest capacity of maintaining PDL cell viability at 1 h and 24 h. IAW showed a statistically significantly lower percentage of viable cells at all three test periods as compared to 7% PE. After 3 h, 30% AVE demonstrated maximum viable cells. Conclusions: Within the limitations of this study, propolis at a concentration of 7% was the most effective medium for maintaining PDL cell viability.

An In Vitro Evaluation of Ice Apple as a Novel Storage Medium to Preserve the Viability of Human Periodontal Ligament Fibroblasts.

Aim: For a better prognosis of an avulsed tooth, it is important to store it in an appropriate storage medium prior to replantation. The purpose of this study was to evaluate the ability of ice apples to preserve the viability of periodontal ligament (PDL) fibroblasts. Materials and methods: Periodontal ligament (PDL) fibroblasts were procured from the roots of healthy premolars and cultured in Dulbecco's Modified Eagle's Medium (DMEM). They were preserved with-ice apple water (IAW), 30 and 10% ice apple fruit pulp extract (IAFPE), DMEM, negative control without any agent, and positive control DMEM supplemented with fetal bovine serum (FBS). Culture plates were incubated with investigational media at 37°C for 1, 3, and 24 hours. Each experiment was repeated thrice. Assessment of cell viability was done using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium reduction (MTT) assay. After every test period, storage media was removed; 60 µL of MTT solution was added to each well and incubated for 3 hours at 37°C. The supernatant was aspirated and the formazan blue crystals formed were solubilized using dimethyl sulphoxide (DMSO) (100 µL). Optical density was measured at a wavelength of 490 nm. The effects of the test storage media at each time period were evaluated by a one-way analysis of variance (ANOVA) test, and this was followed by post hoc Tukey's multiple comparison tests (p < 0.05). Results: A total of 10% IAFPE demonstrated the maximum capacity to maintain PDL cell viability in all three test periods (p = 0.001). Among the different forms of ice apple used in this study, IAFPE showed better results as compared to IAW (p = 0.001). Conclusion: Ice apple fruit pulp extract (IAFPE) at 10% concentration showed the maximum capacity to maintain PDL cell viability across all three test periods. Therefore, it can be considered a suitable alternative natural storage medium for avulsed teeth. However, further, more detailed studies are required in this field. How to cite this article: Bijlani S, Shanbhog RS. An In Vitro Evaluation of Ice Apple as a Novel Storage Medium to Preserve the Viability of Human Periodontal Ligament Fibroblasts. Int J Clin Pediatr Dent 2022;15(6):699-703.

Secular trends in prevalence of overweight and obesity over a decade in urban and rural South Indian children integrated with geographic information system.

Context: The globalization in the process most of the rural sectors are also upgrading technically with digitalization with more availability of smart phones and gaming along with the refined food influences on the present-day children is causing a trend of overweight among the children. Aims: To arrive at the prevalence of overweight and obesity considering the Indian Academy of Pediatrics (IAP) cut-offs for growth standards among children aged 12 years from the rural and urban areas of Mysuru District. Methods and Material: The present program was the third large scale community intervention project carried out including the 7 talukas of Mysuru District which focused on the measurement of the Body mass index (BMI). In this phase between the year 2019 and 2020 anthropometric measurements were undertaken in 1602 urban and rural children aged 12 years from government-funded and privately-funded schools of Mysuru District. Statistical Analysis Used: The results were analysed statistically using SPSS for Windows (version 23.0) and the t-test, Chi-square test and proportion tests, P value were reported. Results: As per the IAP reference ranges the prevalence of obesity was 5.5% and overweight was 7.1% among children of Mysuru District. Conclusions: We report that this increase was significantly higher in girls than boys. There was an increasing trend in rural sectors; however, the obese children were more predominantly distributed among the urban sectors. This calls for a precise preventive measure to fulfil the goal of the World Health Organization (WHO) to control prevalence of childhood obesity by the year 2022.

Knowledge, attitude, and practices of grassroot health workers about early childhood caries.

OBJECTIVE: This study examines the knowledge levels pertaining to early childhood caries (ECC) among grassroot workers of rural India. DESIGN: A questionnaire study assessing the awareness levels of various ECC aspects was carried out among 88 auxiliary nurse midwives (ANMs), 140 Anganwadi workers (AWWs), and 210 Accredited Social Health Activist (ASHA) workers in a district of Southern India. A 24-item pretested, structured, and close-ended, self-administered questionnaire was used to collect information on the knowledge of early childhood oral health-related factors. Descriptive statistics and multivariate ANOVA were used to summarize the results. RESULTS: Overall, only 59% of ANMs, 49% of AWWs, and 47% of ASHA workers were found to have adequate knowledge about ECC. CONCLUSION: The current study clearly highlighted the discrepancy in the awareness levels among grassroot health care workers. A deficit was found to exist in the knowledge of the tested health care workers regarding infant oral care and ECC transmissibility.

Resin infiltration and remineralization interventions in management of moderate dental fluorosis: A quantitative light-induced fluorescence-based randomized controlled trial.

CONTEXT: Aesthetic concerns due to dental fluorosis (DF) considerably impact pyschosocial health in young children and requires aesthetic microinvasive treatment. AIMS: The aim of this study was to use quantitative light-induced fluorescence to evaluate two intervention protocols: microabrasion with resin infiltration and microabrasion with remineralization and assess which brings about more fluorescence gain; better colour masking and greater patient satisfaction among subjects with dental fluorosis. METHODS AND MATERIAL: 120 maxillary central incisors with Grade 3 DF (Dean's Fluorosis Index) were randomly distributed into four groups: I-microabrasion + resin infiltration (fluorosis with stains); II microabrasion + remineralization (fluorosis with stains); III-microabrasion + resin infiltration (fluorosis without stains); IV microabrasion + remineralization (fluorosis without stains). At baseline, post-microabrasion and post-intervention Quantitative light-induced fluorescent images were captured. Fluorescence gain (ΔF) was analysed from the blue light images. The white light images were used to evaluate post intervention colour difference (ΔE = [(ΔL*) 2+(Δa*) 2+(Δb*) 2]1/2) and child satisfaction was evaluated. STATISTICAL ANALYSIS USED: Collected data was analysed using repeated measures ANOVA and Independent t-test. RESULTS: Intra-group comparison of ΔF values showed statistically significant improvement in ΔF value in all the four groups (P < 0.001, 0.002). Inter-group comparison of ΔF values based on intervention showed statistically significant fluorescence gain (P < 0.004) indicating resin infiltration intervention being better than remineralization in unstained grade III fluorosis. The colour difference was statistically significantly better with resin infiltration in both stained and unstained Grade III DF (P < 0.001). CONCLUSIONS: Resin infiltration intervention showed better fluorescence gain and colour masking compared to remineralization intervention.

Validation of cone beam computed tomography-based classification method for individual assessment of mid-palatal suture with respect to clinical application among Indian children.

AIM: : The aim of this study is to validate the Fernanda Angelieri classification method for the individual assessment of mid-palatal suture among Indian children using multi-slice computed tomography (CT) with respect to clinical application in rapid maxillary expansion. MATERIAL AND METHODS: Present study was conducted utilizing 760 existing head and neck CT image records. CT images were stratified into three categories based on growth spurts: Group I - 6-10 years (n = 210), Group II - 11-14 years (n = 270), and Group III - 15-18 years (n = 280). The CT images were analyzed for stages of mid-palatal suture maturation, according to age and sex. The recorded data were subjected for statistical analysis. RESULTS: Indian children, up to age of 10 years, were distributed in stage A and B. After 11 years, up to 14 years girls showed varied distribution and spread equally among stage B, C, and D. After 15 years, more number of girls were distributed in stage D and E, whereas boys remained distributed variedly in stage B, C, and D. Mid- palatal suture maturational stages correlated to chronological age among both the genders using Fisher exact test and expected contingency table showed statistically significant variation among both the gender independently and collectively (P < 0.001). CONCLUSION: Cone beam computed tomography (CBCT)-based prediction of stages of mid- palatal suture maturation by Fernanda Angelieri is valid among the Indian population. During treatment plan of maxillary expansion among children above 10 years, it is better to have a diagnostic CBCT image analysis of mid-palate suture for predicable prognosis.

Evaluation of mineral loss in primary and permanent human enamel samples subjected to chemical demineralization by international caries detection and assessment system II and quantitative light-induced fluorescence™: An in vitro study.

PURPOSE: The purpose of the study was to understand the mineral loss in primary and permanent enamel samples and an attempt is made to standardize the process of chemical demineralization to generate more meaningful results in research studies involving the remineralization of demineralized samples. MATERIALS AND METHODS: Due to variability among enamel samples theoretically, it is impossible to standardize demineralization by running time-based chemical demineralization cycle without frequent monitoring. Instead of carrying out demineralization cycles for a fixed duration of time, we quantified the mineral loss 24 hourly using the International Caries Detection and Assessment System (ICDAS) and Quantitative Light-induced Fluorescence System (QLF™). Twenty primary and permanent enamel samples were subjected to demineralization, and ICDAS and QLF™ evaluation were done at 0, 24, 48, 72, 96, 120, 144, and 168 h of demineralization. RESULTS: The first visual change in permanent enamel is appreciated at 24 h (ICDAS II code1, QLF™ code1 -16.353 - ΔF) of demineralization, at 48 h (ICDAS II code2, QLF™ code2, -24.515 - ΔF), there was localized white spot lesion in permanent enamel and remained until 96 h (ICDAS II code 2, QLF™ code 2, -25.739 - ΔF) of demineralization. In primary samples, distinct visual change was seen at 24 h (ICDAS II code2, QLF™ code2, -19.431 - ΔF), and at 48 h clinically, there was a distinct visual change, but optically mild enamel breakdown was appreciated (ICADSII code 2 QLF™ code3, -27.201 - ΔF), which remained constant till 120 h of demineralization (ICDAS II code2 QLF™ code3 -37.645 - ΔF). CONCLUSION: Different samples demineralize at different rates. The demineralization in primary samples was 1.25 times higher than permanent samples. Recommendation: due to inherent variability in the samples continuous monitoring of the demineralization process on a 24 hourly basis is required to standardize the process.

Interceptive management of ectopically erupting central incisor - A case report.

Radicular cysts are considered to be rare in the primary dentition comprising 0.5%-3.3% of the total number of radicular cysts in both primary and permanent dentition. Ectopic eruption is an eruption disturbance with the prevalence of 5.6% with majority being permanent central incisors. Etiology for eruption disturbance commonly includes odontoma, cysts, supernumerary teeth, and crown-root malformation. Ectopic eruption of permanent incisor due to radicular cyst associated with traumatized primary incisor is a very rare clinical entity that requires timely interception. The present case illustrates the clinical feature and multi-disciplinary management of ectopic eruption of permanent central incisor in a 9-year-old girl child.

Fluoride ingestion and thyroid function in children resident of naturally fluoridated areas - An observational study.

BACKGROUND: Literature shows association between systemic fluorides with water fluoride level above 3ppm and endocrine disorders especially related to thyroid. Aim & Objectives: To estimate serum T3, T4, TSH, Fluoride levels among children with normal nutritional status and optimal iodine intake, residing in three different ranges of drinking water fluoride levels below 3ppm. MATERIAL AND METHODS: Present double blinded observational trial comprised of 293 children aged between 9-13 years consuming naturally fluoridated water of 3 different ranges: Group I: 0.01 - 0.6ppm, Group II: 0.7-1.2ppm and Group III: 1.3-1.8ppm. For each child demographic data, BMI and Clinical Fluorosis Index were recorded along with serum T3, T4, TSH, Fluoride levels. Data was analyzed using Chi Square, Kruskal Wallis test and Repeated measures of ANOVA with SPSS 23. RESULTS: For serum TSH levels 40% of children of group I had deranged levels followed by group III (20%) and Group II (16%). For serum T4 levels 24% of children of both groups I and III had deranged levels followed by group II (20%). Inter group correlation of drinking water fluoride levels to number of deranged serum T3, T4, and TSH of the children showed non-significant association. CONCLUSIONS: According to the present study results long term intake of fluoridated drinking water (0.02 -1.4 ppm) did not showed effect on the thyroid function in the children with normal nutritional status and optimal iodine intake. Key words:Iodine, nutrition, serum fluoride, systemic fluoride, thyroid function test.

Fluoride and Thyroid Function in Children Resident of Naturally Fluoridated Areas Consuming Different Levels of Fluoride in Drinking Water: An Observational Study.

BACKGROUND: Literature shows association between systemic fluorides and endocrine disorders especially related to thyroid, with lack of clarity. AIMS AND OBJECTIVES: The aim and objective of this study was to estimate serum triiodothyronine (T3), thyroxin, thyroid-stimulating hormone (TSH), fluoride, calcium, phosphate, and alkaline phosphatase levels among children with normal nutritional status and optimal iodine intake residing in three different ranges of drinking water fluoride levels. MATERIALS AND METHODS: The present double-blinded, observational trial comprised of 293 children aged between 9 and 13 years consuming naturally fluoridated water of three different ranges: Group I: 0.01-0.6 parts per million (ppm), Group II: 0.7-1.2 ppm, and Group III: 1.3-1.8 ppm. For each child's demographic data, body mass index and Clinical Fluorosis Index were recorded along with serum T3, T4, TSH, fluoride, calcium, phosphate, and serum alkaline phosphatase levels. Data were analyzed using Chi-square test, Kruskal-Wallis test, and repeated measures ANOVA with SPSS 23. RESULTS: For serum TSH levels, 40% of children in Group I had deranged levels followed by Group III (20%) and Group II (16%). For serum T4 levels, 24% of children of both Groups I and III had deranged levels followed by Group II (20%). Intergroup correlation of drinking water fluoride levels to the number of deranged serum T3, T4, and TSH of the children showed nonsignificant association. Serum T3, calcium, phosphate, and alkaline phosphatase levels in all children showed values falling within normal range. CONCLUSION: According to the present study results, long-term intake of fluoridated drinking water (0.02-1.4 ppm) did not show effect on the thyroid function in children with normal nutritional status and optimal iodine intake.

Correlation of oral health status of socially handicapped children with their oral heath knowledge, attitude, and practices from India.

BACKGROUND: Information on oral health knowledge and practice in orphanage house children is essential for healthcare policy makers to promote oral health resources and address oral health needs of this unprivileged group of society. OBJECTIVES: To assess the source of information, level of knowledge, attitude, and practice toward oral hygiene and oral health among socially handicapped children from city of Mysore, Karnataka state, India. MATERIALS AND METHODS: A cross-sectional survey was conducted among 488 children of 12-14 years of age living in five different orphanage houses of Mysore district. Data regarding knowledge and practice were collected through structured questionnaire and oral health status by type III clinical oral examinations by two trained examiners. Decayed, Missing, Filled, Teeth (DMFT) and OH I-S was then correlated with the above information. Spearman's correlation test was used to measure the correlation. RESULTS: The final data analysis included 488 children, of which 216 (44.26%) were boys and 272 (55.74%) were girls. A total of 88.5% children showed one or more decayed teeth in their oral cavity, with an overall mean DMFT of 3.55. Among DMF, component D showed maximum value with mean 3.42, followed by components F and M. Correlation between source of information, knowledge, and attitude for oral health to oral hygiene index (OHI-S; P < 0.05) and gingival index (P < 0.001) showed highly significant negative values. Correlation between oral hygiene practice to OHI-S, DMFT, and gingival index (P < 0.001) showed highly significant (P < 0.001) negative values. CONCLUSIONS: Although children of orphanage have positive attitude toward oral health, knowledge and practice among children are still below the satisfactory level. The children did not receive appropriate information or, if informed, were not re-evaluated or reinforced for its practical application by the concerned authority. The findings of this study suggest that awareness on the importance of oral health needs to be enhanced among the orphanage children of Mysore.

Clinical consequences of untreated dental caries evaluated using PUFA index in orphanage children from India.

BACKGROUND: To determine the prevalence and severity of oral condition related to untreated dental caries with PUFA index and to relate period of institutional stay, oral hygiene practice and diet of orphan children to caries experience ratio. MATERIALS & METHODS: A cross sectional survey conducted among 488 children of 12-14 years living in 5 different orphanages of Mysore district, India. Data regarding oral hygiene practices and oral health status (PUFA, DMFT, OH I-S and GI) were collected through structured questionnaire and by type III clinical oral examinations. The collected data were processed statistically. RESULTS: The PUFA ratio indicates 21% of decayed component had progressed to pulp involvement and abscess formation. The overall prevalence of PUFA was 37.7%. 31.1% children showed one or more pulpally involved tooth in their oral cavity. Correlation between periods for being the child in the institute to DMFT showed negative value indicting decrease in DMFT as the duration of stay in orphanage increases. CONCLUSION: The result show oral health condition in orphan children was neglected. Children from this disadvantaged background have shown a high prevalence of dental caries with low dental care utilization. PUFA index is an effective index in evaluating clinical consequences of un-treated caries. How to cite this article: Shanbhog R, Godhi BS, Nandlal B, Kumar SS, Raju V, Rashmi S. Clinical consequences of untreated dental caries evaluated using PUFA index in orphanage children from India. J Int Oral Health 2013; 5(5):1-9.