Effect of Exogenous Plant Debris and Microbial Agents on Phytoremediation of Copper-Contaminated Soil in Shanghai.

Bioaugmentation is an important measure for improving the efficiency of phytoremediation. The objective was to identify the role of exogenous plant debris with different processing and microbial agents for soil characteristics, copper bioavailability and phytoextraction. The experimental design consisted of four blocks, which were divided into 48 plots. Each plot was planted with Fraxinus chinensis and Salix matsudana × alba, which was added to plant composts, woody chips and effective microorganisms (EM) agents, using an orthogonal experimental design. The results showed that the order of bioaugmentation materials on the Cu phytoextraction of two woody species was plant composts > wood chips > EM agents. The best performance of F. chinensis was in the T15 treatment (30% plant composts + 7.5% wood chips + 40 mL·m−2), with phytoextraction of 33.66 mg·m−2, as well as 4.32 mg·m−2 in the T16 treatment (30% plant composts + 15% wood chips) of S. matsudana × alba. Cu was accumulated mainly in the roots of the two woody plants. The phytoextraction of the above-ground parts was promoted by bioaugmentation, due to the improvement in the physical soil characteristics and Cu bioavailability. The phytoextraction performance of F. chinensis was promoted by the improvement in the Cu concentration after treatments, while for S. matsudana × alba, it was the dry biomass. Thus, targeted strengthening measures should be to applied, to improve the efficiency of phytoremediation.

Valuable alkaloids content is preserved in Camptotheca acuminata and Morus alba grown in trace elements contaminated soil.

Phytoextraction of trace elements (TE) using woody species is an economically challenging soil remediation approach because of the long time needed. Yet, some trees contain alkaloids that can be exploited along structural components to enhance biomass value. As alkaloids are thought to be involved in plant defence mechanisms, we hypothesized that potentially hostile phytoremediation conditions could increase their level. Camptothecin in Camptotheca acuminata and 1-deoxynojirimycin in Morus alba were measured from trees grown in a field in presence of Cu, Pb and Zn all together, and from M. alba grown in a greenhouse in presence of Cd or other abiotic stressors (NaCl and bending). The trees did not extract TE in the field, but M. alba stems accumulated Cd in the greenhouse experiment, with no consequence on stomatal conductance and leaves pigments concentration. Camptothecin and 1-deoxynojirimycin concentrations were preserved under all experimental conditions, as was biomass yield, and phenolics were slightly increased in M. alba exposed to TE. This study provides evidence that valuable and persistent alkaloids and phenolics can be extracted from trees facing phytoremediation-associated stresses, without a negative impact on their quantity and on biomass yield. Such products could generate a sustainable stream of revenues during phytoremediation.

There is scarce data on tree alkaloid content and scarcer data on how it is affected by exposure to trace elements in a phytoremediation context. We provide evidence that the content of two specific alkaloids is not altered in Morus alba and Camptotheca acuminata exposed to moderate to elevated levels of contaminating trace elements. The manuscript introduces the use of M. alba for phytoremediation in the Americas and is the first to propose the use of C. acuminata on trace element contaminated sites to produce camptothecin, a valuable anticancer alkaloid.

Urban growth drives trait composition of urban spontaneous plant communities in a mountainous city in China.

Urban ecosystems feature intense anthropogenic activities and environmental stressors that filter species with varying life-history traits. The traits therefore provide an essential aspect to understanding how species respond to urban environments. We conducted this study in Chongqing, a mountainous city in southwestern China, and tested the hypothesis that the velocity of urban growth can alter functional compositions of urban plant communities through selection on species' taxonomic distributions and functional traits. We found that for most traits, their values spanned a wide range across the 70 spontaneous species in this study, and seed size and leaf element composition played a key role in contributing to the functional differentiation among species. At the same time, urban growth intensity was negatively correlated with leaf N concentration, the N:P ratio, and specific leaf area (SLA), and positively correlated with the leaf C:N ratio. This suggests that species in urban centers are associated with an acquisitive nutrient-use strategy and may gain strong competitive strategies to be favored by greater selective pressure in those long-term urban centers. Lastly, we show that urbanization as a strong filter tends to reduce the chance of species with unique traits for the spontaneous plant communities. Our study offers insights into mechanisms through which spontaneous plant communities are filtered by urbanization with a special focus on the ecological consequences of the velocity of urban growth.

The use of willow microcuttings for phytoremediation in a copper, zinc and lead contaminated field trial in Shanghai, China.

A phytoremediation approach using willow microcuttings was tested on a large experimental site spiked with Cu, Pb and Zn. The objective was to verify if this approach allows plant establishment and effective extraction of contaminants. The experimental design consisted of four blocks divided into three plots, in which treatments (salts of the three metals) were randomly applied. Each plot was also subdivided to verify the effect of adding diverse compost treatments: no compost, vegetable garden waste compost (Compost 1), food waste compost (Compost 2) and a mix of Composts 1 and 2. Willow microcuttings (Salix matsudana × alba) were scattered on the plots at a density of 60 m-2. Nine months after the implementation, the number of stems developed, yield and concentration of metals in the aerial parts of the willows were measured. The presence of a contaminant did not affect the number of stems developed. However, the presence of Compost 1 had a significant effect on the number of stems produced as well as on the biomass. Among the three metals, Zn was the element found in highest concentration in willow tissues. An estimated minimum 300 g of Zn per hectare per year could be removed using this method.

Biomass and phytoextraction potential of three ornamental shrub species tested over three years on a large-scale experimental site in Shanghai, China.

Issues related to environmental degradation are of increasing concern worldwide. In urban Shanghai, many plant species used for ornamental purposes grow under harsh conditions yet show good resistance to pollution. Twelve shrub species were tested in a previous study to evaluate their capacity to tolerate and absorb inorganic contaminants. Among these, Hibiscus mutabilis and H. hamabo and Senna corymbosa, presented good performance and were tested over three growing seasons in a large experimental design (2000 m2) where the soil was spiked with salts of Cu, Pb, or Zn. Each year of the study, all plants were cut and biomass was harvested and analyzed. Despite the relatively high concentration of metals in plots, no signs of toxicity were observed. Concentrations of metals in root tissues were generally much higher than those found in aerial parts. The bioconcentration factor values were generally very low, but the high biomass yield produced by H. mutabilis led to significant removal of Cu and Zn. No difference in the quantity of Pb extracted was found between species. As these plants respond well to coppicing, it may be possible to gradually eliminate contaminants from soils. Their use can also embellish the landscape while generating many other ecological services.

Plant growth and nutrient uptake in treatment wetlands for water with low pollutant concentration.

The objective of this study was to determine how macrophytes commonly used in treatment wetlands (TWs) respond to water with low pollutant concentration. We measured pollutant removal efficiency and compared growth and nutrient uptake of five macrophytes in demonstration scale units (volume >40 m3) irrigated by water with pollutant concentrations representative of average urban stormwater quality. All species showed a strong productivity gradient along the beds, starting with high biomass - high density near the inlet, then decreasing progressively with distance. Cyperus was by far the most productive species. Phragmites and Thalia had higher biomass in the first few metres of the beds than Typha and Arundo. In terms of pollutant removal, decreasing plant growth may be interpreted as indicative of high efficiency when caused by nutrient depletion. Differences in aboveground biomass between species did not translate into measurable differences in removal efficiency at the outlet. Although Phragmites australis is the species most commonly used in TWs, under the low nutrient load, Cyperus had twice its biomass, and higher N and P uptake. These results highlight the importance of considering wastewater characteristics when selecting macrophyte species for TWs.