Dynamics of the changes in homogenate respiratory activity after rat whole liver storage at 4 degrees C.

Homogenate respiratory activity was studied after different storage terms of the whole rat liver at 4 degrees C in sucrose-based solution and following normothermic reperfusion. Preservation of homogenate respiratory activity in all metabolic states after normothermic reperfusion of the control liver (60 min, 4 degrees C) is shown. Further storage (6 and 24 hrs) of isolated liver under the mentioned above conditions strengthens the substrate respiration of homogenate both after storage and after normothermic reperfusion. At the same time oxidative phosphorylation does not practically change. No change was noted in respiratory activity in the states 3, 4ADP and 3DNP after 24 hrs of liver storage in respect of a previous term. Following normothermic liver reperfusion contributes to a statistically true reduction of mentioned parameters of respiration, that correlates with a decrease in the degree of respiration and phosphorylation coupled of the studied system.

A comparison of a sucrose-based solution with other preservation media for cold storage of isolated hepatocytes.

A sucrose-based solution has been compared with other preservation solutions (University of Wisconsin (UW) solution and Marshall's citrate solution, with Dulbecco's medium as control) during hypothermic preservation of isolated rat hepatocytes for up to 72 h. Studies on the stability of liver cells at low temperature by exclusion of trypan blue dye and morphological appearance were conducted. During storage beyond 24 h, there was a clear difference between cells stored in Dulbecco's medium and Marshall's citrate and those stored in sucrose-based solution and UW solution, with the former storage groups showing many cells developing large membrane "blebs" and the latter storage groups maintaining a more typical morphology and developing only small membrane protrusions. Dye exclusion was higher in sucrose-based solution (48 h, 75 +/- 7%; 72 h, 65 +/- 6%) and UW solution (48 h, 72 +/- 5%; 72 h, 63 +/- 4%) than in Marshall's citrate (48 h, 31 +/- 5%; 72 h, 10 +/- 1%) and Dulbecco's medium (48 h, 8 +/- 2%; 72 h, 5 +/- 1%). These data suggest that sucrose-based solution should be investigated further as a less complex alternative solution for storage of isolated hepatocytes.

[The effect of hypothermia on certain biochemical indicators of isolated hepatocytes]. / Vliianie gipotermii na nekotorye biokhimicheskie pokazateli izolirovannykh izolirovannykh gepatotsitov.

The media containing nonpenetrating components (0.25 M sucrose, BSA) and antioxidant (silibor) are able for a long time to preserve at 4 degrees C a great number of liver cells. Survival of the same hepatocytes after normothermic incubation indicated a good condition of the mitochondrial system both at the stage of isolation and after hypothermic storage. Thus, by inhibiting osmotic impairment, which developed under hypothermic storage, we produced a prolongation of the time of "ageing-death" of an isolated liver cell at 4 degrees C. The addition of a drug possessing antioxidant properties was also beneficial.