RESUMO
Objective The effect of different oxygen tensions on the cytoskeleton remodeling of goat temporomandibular joint (TMJ) disc cells were investigated. Methods Goat TMJ disc cells were cultured under normoxia (21% O2) and hypoxia (2%, 4%, and 8% O2). Toluidine blue, picrosirius red, and type â collagen immunocytochemical staining were performed to observe the changes in cell phenotype under different oxygen levels. Immunofluorescent staining and real-time reverse transcription-polymerase chain reaction analysis were then performed to identify actin, tubulin, and vimentin in the cultured disc cells. Results TMJ disc cells still displayed fibroblast characteristics under different oxygen levels and their cytoskeletons had regular arrangement. The fluorescence intensities of actin and vimentin were lowest at 4% O2(P<0.05), whereas that of tubulin was highest at 2% O2 (P<0.05). No significant difference among the other groups was observed (P>0.05). Actin mRNA levels were considerably decreased at 2% O2 and 4% O2 in hypoxic conditions, while actin mRNA expression was highest in 21% O2. Tubulin mRNA levels considerably increased at 2% O2, while tubulin mRNA expression was lowest in 8% O2 (P<0.05). Vimentin mRNA expression was lowest at 4% O2 and highest at 21% O2, and significant differences were observed between vimentin mRNA expression levels among these oxygen levels (P<0.05). Conclusion Cytoskeletons were reconstructed in different oxygen tensions, and 2% O2 may be the optimal oxygen level required to proliferate TMJ disc cells.
