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Yi Chuan ; 31(2): 199-205, 2009 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-19273429

RESUMO

NAC transcription factors are a family of functionally diverse proteins. They are unique to plants and play an important role in regulation of plant growth and development, hormone regulation and responses to various stresses. A cDNA encoding the NAC-like gene homologue was isolated from maize (Zea mays L.) by RT-PCR and designated ZmNAC1 (GenBank Accession No. EU224278). Sequence analysis showed that cDNA of ZmNAC1 was 1,029 bp long and contained a single open reading frame (ORF, 26 to approximately 907 bp). The predicted ZmNAC1 protein has 293 amino acids with an estimated molecular mass of 32.3 kDa and an isoelectric point of 8.65. RT-PCR analysis showed that the expression of ZmNAC1 was induced by low temperature, PEG, salt, and ABA, respectively. These results suggest that ZmNAC1 may play important roles in biotic and abiotic resistance pathways. This is the first NAC-like gene reported in maize.


Assuntos
Regulação da Expressão Gênica de Plantas , Fatores de Transcrição/genética , Zea mays/genética , Sequência de Aminoácidos , Proteínas de Arabidopsis , Sequência de Bases , Clonagem Molecular , DNA Complementar/análise , DNA Complementar/química , Dados de Sequência Molecular , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Alinhamento de Sequência , Transativadores , Fatores de Transcrição/metabolismo , Zea mays/metabolismo
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