RESUMO
Thermotoga maritima is strictly anaerobic and extremely thermophilic bacteria. The endoglucanase found in T. maritima showed extremely high thermostability and considerable potential in industrial application. Endoglucanase (Tm) Cel12B is extracellular enzyme. Tm Cel12B did not contain a cellulose-binding domain (CBD)and lacked activity on crystalline cellulose. Tm XynA is composed of catalytic domain (CD) and cellulose-binding domain (CBD). As such, the gene of CBD from Tm XynA was fused at the carboxyl-terminus of Tm Cel12B and recombinant plasmid pET-20b- Cel 12B- CBD was obtained. The recombinant plasmid pET-20b- Cel 12 B- CBD was transformed to E. coli JM109 (DE3), induced by IPTG. The properties of chimeric enzyme were determined. The chimeric enzyme displayed pH activity and stability profiles similar to those of parental enzyme with optimal pH 5.8. The optimal activity of the chimera was observed at 100 degrees C and the enzyme kept 87% of original enzyme activity after incubated at 90 degrees C for 2h. A notable feature on substrate specificity is that the chimeric enzyme has the capacity to hydrolases crystalline cellulose.
Assuntos
Fusão Gênica Artificial , Celulase/genética , Celulose/metabolismo , Endo-1,4-beta-Xilanases/genética , Proteínas Recombinantes de Fusão/biossíntese , Thermotoga maritima/enzimologia , Sítios de Ligação , Endo-1,4-beta-Xilanases/química , Estabilidade Enzimática , Concentração de Íons de Hidrogênio , TemperaturaRESUMO
An efficient and economical medium--Thermotoga maritima basal medium (TMB)--was designed for the cultivation of T. maritima under either liquid or solid conditions. When the broth was flushed with N2 or CO2 throughout cell growth in a 10-L fermentor (pH controlled to 6.5), the maximum cell density (OD600) on TMB containing 1% glucose rose to 2.0 or higher (1.63 x 10(9) cells mL(-1)). Sheath-less cells observed by electron microscopy were captured during growth in the fermentor. Using a two-layer plating method, isolated single-well colonies were consistently obtained within 24 h on the TMB in modified tissue culture flasks. The minimal inhibitory chloramphenicol concentrations for T. maritima on TMB agar were 5 microg mL(-1) after 24 h and 48 h, and 25 microg mL(-1) at 72 h.
Assuntos
Técnicas Bacteriológicas/métodos , Meios de Cultura , Thermotoga maritima/crescimento & desenvolvimento , Anaerobiose , Antibacterianos/farmacologia , Metabolismo dos Carboidratos , Contagem de Colônia Microbiana , Meios de Cultura/química , Fermentação , Temperatura Alta , Testes de Sensibilidade Microbiana , Microscopia Eletrônica , Thermotoga maritima/citologia , Thermotoga maritima/efeitos dos fármacos , Thermotoga maritima/metabolismoRESUMO
An extracellular alkaline protease produced by Bacillus licheniformis AP-1 was purified 76-fold, yielding a single 28 kDa band on SDS-PAGE. It was optimally active at pH 11 and at 60 degrees C (assayed over 10 min). The protease was completely inhibited by phenylmethylsulfonyl fluoride and diodopropyl fluorophosphate, with little increase upon Ca2+ and Mg2+ addition.
Assuntos
Bacillus/enzimologia , Serina Endopeptidases/química , Serina Endopeptidases/isolamento & purificação , Bacillus/classificação , Ativação Enzimática , Estabilidade Enzimática , Concentração de Íons de Hidrogênio , Indústrias , Peso Molecular , Serina Endopeptidases/análise , Especificidade da Espécie , TemperaturaRESUMO
The alkaline protease gene, apr, from Bacillus licheniformis 2709 was cloned into a Bacillus shuttle expression vector, pHL, to yield the recombinant plasmid pHL-apr. The pHL-apr was expressed in Bacillus subtilis WB600, yielding a high expression strain BW-016. The amount of alkaline protease produced in the recombinant increased by 65% relative to the original strain. SDS-PAGE analysis indicated a Mr of 30.5 kDa. The amino acid sequence deduced from the DNA sequence analysis revealed a 98% identity to that of Bacillus licheniformis 6816.
Assuntos
Bacillus/enzimologia , Bacillus/genética , Engenharia de Proteínas/métodos , Serina Endopeptidases/biossíntese , Serina Endopeptidases/química , Sequência de Aminoácidos , Bacillus/classificação , Bacillus subtilis/enzimologia , Bacillus subtilis/genética , Clonagem Molecular/métodos , Regulação Bacteriana da Expressão Gênica/fisiologia , Regulação Enzimológica da Expressão Gênica/fisiologia , Dados de Sequência Molecular , Peso Molecular , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/genética , Homologia de Sequência de Aminoácidos , Serina Endopeptidases/classificação , Serina Endopeptidases/genética , Especificidade da EspécieRESUMO
The xylanolytic enzymes found in Thermotoga maritima showed extremely high thermostability and considerable potential in industrial application. Yet expression level of the genes encoding these enzymes was very low. The alpha-glucuronidase gene aguA from T. maritima ATCC 43589 was cloned and expressed in several E. coli strains with different vector. The alpha-glucuronidase was overexpressed in E. coli BL21-CodonPlus(DE3)-RIL with plasmid pET-28a(+), and made up about 20% of the total proteins present in the intracellular soluble fraction. The results proved the assumption that rare codons for arginine (AGA/AGG) and isoleucine (AUA) affect the expression of aguA gene from hyperthermophilic bacterium T. maritima in E. coli. Purification procedure included two steps, heat treatment and immobilized metal affinity chromatography, and over 13.5mg of pure enzyme was obrained from 1L of induced culture. The purified enzyme showed a single band on SDS polyacrylamide gel electrophoresis with a purification of 5.1 fold, and a yield of 55.1%. The optimum activity of recombinant alpha-glucuronidase was found at pH 6.0 and 85 degrees C, the enzyme retained 70% of its activity after 1 h of incubation at 85 degrees C. The induction conditions for expression of recombinant strain BL21-CodonPlus(DE3)-RIL/pET-28a-aguA were studied on induction time and duration by IPTG. The results showed that the activity of thermostable alpha-glucuronidase reach the maximum in 5-hour after inducted at the exponential phase (OD600 of 0.7 - 0.8).
Assuntos
Glicosídeo Hidrolases/genética , Proteínas Recombinantes/biossíntese , Thermotoga maritima/enzimologia , Escherichia coli/genética , Glicosídeo Hidrolases/isolamento & purificação , Glicosídeo Hidrolases/metabolismo , Plasmídeos , Proteínas Recombinantes/isolamento & purificaçãoRESUMO
In a 5L fermentor the production conditions of alkaline protease gene engineering strain BA071 were investigated. The maximum activity of alkaline protease reached 24,480 u/mL in 40 hours of fermentation by combination of enhancing aeration and changing the agitation rate. The fast purification method of recombinant protease was conducted with FPLC (Fast Protein Liquid Chromatography). The crude enzyme, treated with ammonium sulfate fractionation and decolored with DEAE-A-50 and polyethylene glycol concentration, was purified with CM-Sephadex-C-50 and Sephadex-G-75. The purified enzyme appears homologous on SDS-PAGE. The purity of enzyme was increased 76.2 times. SDS-PAGE analysis showed that the molecular weights of expressed recombinant products were about 28 kD. The optimal reaction pH and temperature of recombinant enzyme were at pH11 and 60 degrees C, respectively. The recombinant enzyme exhibited high temperature tolerance and was stable at a wide range of pH. Ca2+, MG2+ can enhance the stability of the recombinant enzyme. While the protease activity of the enzyme was strongly inhibited by Hg2+, Ag+, PMFS [symbol: see text] DFP, and was not affected by SDS and Urea.