Construction of the comprehensive evaluation system of waterbody pollution degree and the response of sedimentary microbial community.

This study proposed and established a comprehensive evaluation system for the pollution degree of the waterbody by taking overlying water and sediment as a whole. By dividing different sampling points into three gradients according to the pollution degree, the changes in sedimentary microbes under various pollution gradients were compared. The results showed that microbial diversity, abundance and specific OTUs decreased significantly with the increase in pollution degree. Meanwhile, Firmicutes, Bacteroidota and Caldiseriota increased in the severely polluted group, while Chloroflexi and Acidobacteriota decreased. Spearman correlation analysis and co-occurrence network revealed that COD, pH in overlying water, and Mn, Fe in sediments were the most significant pollution degree evaluation indicators affecting sedimentary microorganisms, which drove the sedimentary microbial communities dominated by Proteobacteria and Firmicutes. FAPROTAX functional prediction indicated that increased pollution levels led to the weakening of functional genes related to nitrogen metabolism and sulfur metabolism and the increase of functional genes related to carbon metabolism in sediment microorganisms. This study not only provided new insights into waterbody pollution evaluation but also verified the feasibility of this evaluation method by the response of sedimentary microbial communities to different pollution degrees.

Enhancement of black and odorous water treatment coupled with accelerated lipid production by microalgae exposed to 12C6+ heavy-ion beam irradiation.

In this study, Auxenochlorella protothecoides (AP-CK) was selected due to its reported high growth potential in sterilized black and odorous water (SBOW). In order to improve the resource utilization level of microalgae for wastewater treatment, AP-CK was mutated using 12C6+ heavy-ion beam irradiation, and a high lipid-containing mutant (AP-34#) was isolated and further evaluated to treat original black and odorous water (OBOW). Compared with the wild type, the maximum removal rates of COD, NH4+-N and TP of the mutant increased by 8.12 ± 0.33%, 10.43 ± 0.54% and 11.97 ± 0.16%, respectively, while maximum dissolved oxygen content increased from 0 to 4.36 ± 0.25 mg/L. Besides, the mutant lipid yield increased by 115.87 ± 3.22% over the wild type in OBOW. The fatty acid profile of AP-34# grown in SBOW and OBOW showed higher proportion of saturated fatty acids (C16:0 and C18:0) and valuable polyunsaturated fatty acids (mainly C20:5n3 and C22:6n3) which are more suitable for biodiesel production and value-added products, respectively. This work provides a new perspective on improving the characteristics of microalgae and an innovative approach for resource-based microalgae wastewater treatment through bioremediation of black and odorous water.

The imbalance of Th17/Treg cells is involved in the progression of nonalcoholic fatty liver disease in mice.

BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) is a common, chronic liver disease worldwide. Recent studies have shown that T helper (Th) 17 and regulatory T (Treg) cells play critical roles in various disorders of liver inflammation. Here, we explored the value of polyene phosphatidylcholine capsules (PPC) for regulating the imbalance of Th17/Treg cells in the pathogenesis of mice with NAFLD. METHODS: C57BL/6 mice were randomly divided into three groups as follows:normal diet (ND), high-fat diet (HF),and HF plus PPC(HF + PPC). The frequencies of splenic Th17 and Treg cells were measured by flow cytometry, and their related cytokines were analyzed by CBA and real-time PCR. RESULTS: At the end of 24 weeks, mice in the HF group had a higher frequency of intrahepatic Th17 cells,and a lower proportion of Treg cells compared with the ND group. The levels of Th17 cell-related cytokines (IL-6, IL-17 and IL-23) in serum and in liver tisse were increased,and the hepatic mRNA levels of RORγt, STAT3 and IL-6 were also increased. By contrast,the FoxP3 mRNA level was decreased in the HF group. Moreover, significant pathological and biochemical changes in the liver, as well as serum biochemical changes, were found in mice with NAFLD. Interestingly, following treatment with PPC, the levels of liver inflammation,frequencies of Th17/Treg cells and associated cytokines,and biochemical data were significantly altered. CONCLUSION: These findings demonstrate a critical role for PPC in partially attenuating liver inflammatory responses in mice with NAFLD that involves the imbalance of Treg/Th17 cells and associated cytokines.