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The tumor microenvironment includes a complex network of immune T-cell subsets that play important roles in colorectal cancer (CRC) progression and are key elements of CRC immunotherapy. T cells develop and migrate within tumors, recognizing tumor-specific antigens to regulate immune surveillance. Current immunotherapies are divided into the following main categories based on the regulatory role of T-cell subsets in the tumor immune microenvironment (TIME): cytokines, monoclonal antibodies, peptide vaccines, CAR-T cells and more. This review describes the composition of the tumor immune microenvironment in colorectal cancer and the involvement of T cells in the pathogenesis and progression of CRC as well as current T-cell-related immunotherapies. Further studies on CRC-specific tumor antigens, the gene regulation of T cells, and the regulation of immune activity are needed.
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AIM:To do cluster analysis on the herb active ingredients with reversal activity of multi-drug resistance in HL60/HT cells.METHODS:HL60/HT cell was used to check the inhibitory effect of Psoralen,psoralen-A,curcumin,quercetin,psoralidin,isopsoralidin,psoralen phenol,Rhein on cell proliferation by MTT assay.Flow cytometry was used to measure HL fluorescence index and the expression of P-glucose protein.On the basis of the experimental data,herbs were classified as different groups by cluster analysis.RESULTS:Reversion rate,HT concentration and P-gp expression of HL60/HT fell within the range of 6.2 to 12.5,9.6 to 25.6,and 29.3 to 50.1,respectively.CONCLUSION:According to cluster similarity,8 herbal ingredients could be divided into three groups,psoralen,curcumin and quercetin were in the first group.Psoralen-A,psoralidin,isopsoralidin and rhein were in the second group,psoralen phenol was in the third group.
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Objective To research the reversal effects of psoralen on multidrug resistant action and its influence on intracellular Ca2+ concentration in MCF-7/ADR cells and to explore its possible mechanisms of reversing multidrug resistance (MDR). Methods The inhibitory effects of psoralen on the viability of MCF-7/ADR cells were determined with MTT assay, intracellular adriamycin (ADR) concentration was assayed with HPLC and the intracellular Ca2+ concentrations in different incubative duration were detected with confocal microscope. Results Psoralen from 1 to 20 μmol/L reduced the value of IC50 of ADR in MCF-7/ADR cells, enhanced accumulation of ADR and influenced Ca2+ concentration with a negative correlation in different duration (24 h, 48 h and 96 h). Conclusion Psoralen can reverse MDR in MCF-7/ADR cells and its mechanism may be related to the increase of the intracellular accumulation of ADR by enhancing the intracellular Ca2+ concentration.
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Objective To research the reversal effects of psoralen on multidrug resistant action and its influence on intracellular Ca2+ concentration in MCF-7/ADR cells and to explore its possible mechanisms of reversing multidrug resistance (MDR). Methods The inhibitory effects of psoralen on the viability of MCF-7/ADR cells were determined with MTT assay, intracellular adriamycin (ADR) concentration was assayed with HPLC and the intracellular Ca2+ concentrations in different incubative duration were detected with confocal microscope. ]Results Psoralen from 1 to 20 ?mol/L reduced the value of IC50 of ADR in MCF-7/ADR cells, enhanced accumulation of ADR and influenced Ca2+ concentration with a negative correlation in different duration (24 h, 48 h and 96 h). Conclusion Psoralen can reverse MDR in MCF-7/ADR cells and its mechanism may be related to the increase of the intracellular accumulation of ADR by enhancing the intracellular Ca2+ concentration.
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Objective To determine the reversal effect of psoralen on multidrug resistance (MDR) in the harringtonine (HT)-resistant leukemic cell line HL60/HT. Method The modulating effect of psoralen on MDR in HL60 and HL60/HT cells were determined by the measurement of cell growth and viability via MTT assay. High performance liquid chromatography(HPLC) was used to analyze intracellular HT concentrations in HL60/HT and HL60 cells treated with psoralen. Result Psoralen from 1 to 20 ?mol/L could reduce the value of IC_ 50 to HT and enhance the accumulation of HT in HL60/HT cells. Conclusion Psoralen can reverse MDR of HL60/HT cells and is a potential modulator of MDR.
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Objective To determine the reversal effect of psoralen on multidrug resistance (MDR) in the harringtonine (HT)-resistant leukemic cell line HL60/HT. Method The modulating effect of psoralen on MDR in HL60 and HL60/HT cells were determined by the measurement of cell growth and viability via MTT assay. High performance liquid chromatography(HPLC) was used to analyze intracellular HT concentrations in HL60/HT and HL60 cells treated with psoralen. Result Psoralen from 1 to 20 μmol/L could reduce the value of IC50 to HT and enhance the accumulation of HT in HL60/HT cells. Conclusion Psoralen can reverse MDR of HL60/HT cells and is a potential modulator of MDR.
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Objective:To study the better extraction method for Qidan decoction.Method:Orthogonal design was used to find befitting conditions.The water quantity,extraction time and frequency were selected as observational conditions.The optimum conditions were repeated three times to study repetition.Results:The optimum extracting procedure is 14 times water, extracting 3 times,the time was 30 min each time.The active ingredient content RSD of three parallel trial is 0.86%.Conclusion: The optimal condition for ultrasonic extraction is 14 times water,extracting 3 times,30 min per time.This method is of good repeatability.
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AIM:To study the rationality and the best dosage of Qidan Decoction (Radix Astragali,Radix et Rhizoma Salviae miltiorrhizae,Fructus Psoraleae,etc.) on inhibiting gastric cancer metastasis. METHODS:The ability of inhibiting gastric cancer metastasis and the activity of NK cell were detected as indexes to appraise the rational and the best dosage. RESULTS:The function of Astragalus membranaceus,Salvia miltiorrhiza.Ege.,psoralea corylifolia and Lycium Barbarum on inhibiting gastric cancer metastasis and rising the activity of NK cell was significant(P
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AIM:To do cluster analysis on the herb active ingredients with reversal activity of multi-drug resistance in HL60/HT cells.METHODS:HL60/HT cell was used to check the inhibitory effect of Psoralen,psoralen-A,curcumin,quercetin,psoralidin,isopsoralidin,psoralen phenol,Rhein on cell proliferation by MTT assay.Flow cytometry was used to measure HL fluorescence index and the expression of P-glucose protein.On the basis of the experimental data,herbs were classified as different groups by cluster analysis.RESULTS:Reversion rate,HT concentration and P-gp expression of HL60/HT fell within the range of 6.2 to 12.5,9.6 to 25.6,and 29.3 to 50.1,respectively.CONCLUSION:According to cluster similarity,8 herbal ingredients could be divided into three groups,psoralen,curcumin and quercetin were in the first group.Psoralen-A,psoralidin,isopsoralidin and rhein were in the second group,psoralen phenol was in the third group.
