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The authors conducted a retrospective and brief prospective study of adverse effects of approximately 350 medications in 44 adults with late-onset Tay-Sachs disease (LOTS). Some medications were relatively safe, whereas others, particularly haloperidol, risperidone, and chlorpromazine, were associated with neurologic worsening.
Assuntos
Psicotrópicos/efeitos adversos , Doença de Tay-Sachs/fisiopatologia , Doença de Tay-Sachs/terapia , Adulto , Idoso , Analgésicos não Narcóticos/uso terapêutico , Progressão da Doença , Eletroconvulsoterapia/métodos , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Estudos RetrospectivosRESUMO
BACKGROUND: Late-onset Tay-Sachs disease (LOTS) is an adult-onset, autosomal recessive, progressive variant of GM2 gangliosidosis, characterized by involvement of the cerebellum and anterior horn cells. OBJECTIVE: To determine the range of visual and ocular motor abnormalities in LOTS, as a prelude to evaluating the effectiveness of novel therapies. METHODS: Fourteen patients with biochemically confirmed LOTS (8 men; age range 24 to 53 years; disease duration 5 to 30 years) and 10 age-matched control subjects were studied. Snellen visual acuity, contrast sensitivity, color vision, stereopsis, and visual fields were measured, and optic fundi were photographed. Horizontal and vertical eye movements (search coil) were recorded, and saccades, pursuit, vestibulo-ocular reflex (VOR), vergence, and optokinetic (OK) responses were measured. RESULTS: All patients showed normal visual functions and optic fundi. The main eye movement abnormality concerned saccades, which were "multistep," consisting of a series of small saccades and larger movements that showed transient decelerations. Larger saccades ended earlier and more abruptly (greater peak deceleration) in LOTS patients than in control subjects; these changes can be attributed to premature termination of the saccadic pulse. Smooth-pursuit and slow-phase OK gains were reduced, but VOR, vergence, and gaze holding were normal. CONCLUSIONS: Patients with late-onset Tay-Sachs disease (LOTS) show characteristic abnormalities of saccades but normal afferent visual systems. Hypometria, transient decelerations, and premature termination of saccades suggest disruption of a "latch circuit" that normally inhibits pontine omnipause neurons, permitting burst neurons to discharge until the eye movement is completed. These measurable abnormalities of saccades provide a means to evaluate the effects of novel treatments for LOTS.
Assuntos
Movimentos Sacádicos , Doença de Tay-Sachs/fisiopatologia , Adulto , Idade de Início , Progressão da Doença , Extremidades/fisiopatologia , Movimentos Oculares , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estimulação Luminosa , Movimentos Sacádicos/fisiologia , Doença de Tay-Sachs/complicações , Doença de Tay-Sachs/epidemiologia , Doença de Tay-Sachs/patologia , Testes Visuais , Acuidade VisualRESUMO
Biologists are increasingly recognising that computational modelling is crucial for making sense of the vast quantities of complex experimental data that are now being collected. The systems biology field needs agreed-upon information standards if models are to be shared, evaluated and developed cooperatively. Over the last four years, our team has been developing the Systems Biology Markup Language (SBML) in collaboration with an international community of modellers and software developers. SBML has become a de facto standard format for representing formal, quantitative and qualitative models at the level of biochemical reactions and regulatory networks. In this article, we summarise the current and upcoming versions of SBML and our efforts at developing software infrastructure for supporting and broadening its use. We also provide a brief overview of the many SBML-compatible software tools available today.
Assuntos
Fenômenos Fisiológicos Celulares , Biologia Computacional/normas , Modelos Biológicos , Linguagens de Programação , Software , Biologia de Sistemas , Terminologia como Assunto , Bioquímica/métodos , Bioquímica/normas , Biologia Computacional/métodos , Regulação da Expressão Gênica/fisiologia , Guias como Assunto , Internacionalidade , Padrões de ReferênciaRESUMO
MOTIVATION: Molecular biotechnology now makes it possible to build elaborate systems models, but the systems biology community needs information standards if models are to be shared, evaluated and developed cooperatively. RESULTS: We summarize the Systems Biology Markup Language (SBML) Level 1, a free, open, XML-based format for representing biochemical reaction networks. SBML is a software-independent language for describing models common to research in many areas of computational biology, including cell signaling pathways, metabolic pathways, gene regulation, and others. AVAILABILITY: The specification of SBML Level 1 is freely available from http://www.sbml.org/
Assuntos
Hipermídia , Armazenamento e Recuperação da Informação/métodos , Metabolismo/fisiologia , Modelos Biológicos , Linguagens de Programação , Vocabulário Controlado , Sistemas de Gerenciamento de Base de Dados , Bases de Dados Factuais , Documentação , Regulação da Expressão Gênica/fisiologia , Modelos Químicos , Software , Design de Software , Terminologia como AssuntoRESUMO
In a computational model of spreading depression (SD), ionic movement through a neuronal syncytium of cells connected by gap junctions is described electrodiffusively. Simulations predict that SD will not occur unless cells are allowed to expand in response to osmotic pressure gradients and K+ is allowed to move through gap junctions. SD waves of [K+]out approximately 25 to approximately 60 mM moving at approximately 2 to approximately 18 mm/min are predicted over the range of parametric values reported in gray matter, with extracellular space decreasing up to approximately 50%. Predicted waveform shape is qualitatively similar to laboratory reports. The delayed-rectifier, NMDA, BK, and Na+ currents are predicted to facilitate SD, while SK and A-type K+ currents and glial activity impede SD. These predictions are consonant with recent findings that gap junction poisons block SD and support the theories that cytosolic diffusion via gap junctions and osmotic forces are important mechanisms underlying SD.
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Depressão Alastrante da Atividade Elétrica Cortical/fisiologia , Junções Comunicantes/fisiologia , Modelos Neurológicos , Simulação por Computador , Citosol/metabolismo , Eletrofisiologia , Osmose , Potássio/metabolismoAssuntos
Fístula Arteriovenosa/patologia , Abscesso Encefálico/patologia , Pneumopatias/patologia , Adulto , Fístula Arteriovenosa/diagnóstico por imagem , Abscesso Encefálico/diagnóstico por imagem , Feminino , Humanos , Pneumopatias/diagnóstico por imagem , Imageamento por Ressonância Magnética , Tomografia Computadorizada por Raios XRESUMO
Hemiatrophy caused by linear scleroderma is a race clinical entity of unknown etiology. We present a patient with an inflammatory myopathy in hemiatrophy resulting from linear scleroderma.
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We present a graphical method for a unifying, quantitative analysis of molecular bonding-force measurements by atomic force microscopy (AFM). The method is applied to interpreting a range of phenomena commonly observed in the experimental AFM measurements of noncovalent, weak bonds between biological macromolecules. The analysis suggests an energy landscape underlying the intermolecular force and demonstrates that many observations, such as "snaps-on," "jumps-off," and hysteresis loops, are different manifestations of a double-well energy landscape. The analysis gives concrete definitions for the operationally defined "attractive" and "adhesive" forces in terms of molecular parameters. It is shown that these operationally defined quantities are usually functions of the experimental setup, such as the stiffness of the force probe and the rate of its movement. The analysis reveals a mechanical instability due to the multistate nature of molecular interactions and provides new insight into macromolecular viscosity. The graphical method can equally be applied to a quantitative analysis of multiple unfolding of subunits of the giant muscle protein titin under AFM.
Assuntos
Microscopia de Força Atômica/métodos , Conectina , Substâncias Macromoleculares , Microscopia de Força Atômica/instrumentação , Proteínas Musculares/química , Ligação Proteica , Desnaturação Proteica , Dobramento de Proteína , Proteínas Quinases/química , Estrutura Quaternária de Proteína , Termodinâmica , ViscosidadeRESUMO
Macromolecular binding forces between single protein-ligand pairs have been directly measured with the Atomic Force Microscope (AFM) in several recent experiments. In a typical measurement, the AFM probe, or cantilever, is attached to the ligand and exerts a disruptive force on the bond between the macromolecular pair while the receptor is held fixed; the probe is then moved away from the substrate until the bond is broken. When the bond actually breaks, the tip is observed to slip; in fact, the ligand is jumping to a new equilibrium point determined purely by the cantilever, as if the receptor had been instantaneously moved to infinity. This "jumping-off" or "minimum rupture force" is determined by measuring cantilever deflection. In a similar manner, the two molecules can be brought together and the "jumping-on" force can be determined. These two measurements will result in different estimates of the binding force due to hysteresis. This hysteresis is caused by a cusp catastrophe in the space defined by probe position and cantilever stiffness. The phenomena of "jumping-off" in macromolecular rupture experiments and "jumping-on" when molecules are brought together occur when the system passes through a saddle-node bifurcation as the probe position is varied. Probe approach and withdrawal result in different post-bifurcation equilibria, different energy dissipation, and different force measurements.
Assuntos
Proteínas/metabolismo , Animais , Fenômenos Biomecânicos , Substâncias Macromoleculares , Microscopia de Força Atômica , Modelos Biológicos , Dinâmica não Linear , Ligação ProteicaAssuntos
Esclerose Lateral Amiotrófica/diagnóstico , Eletrodiagnóstico , Músculos Faciais/fisiopatologia , Músculos da Mastigação/fisiopatologia , Língua/fisiopatologia , Idoso , Idoso de 80 Anos ou mais , Esclerose Lateral Amiotrófica/fisiopatologia , Eletromiografia , Estudos de Avaliação como Assunto , Músculos Faciais/inervação , Humanos , Músculos da Mastigação/inervação , Pessoa de Meia-Idade , Degeneração Neural , Regeneração Nervosa , Estudos Prospectivos , Língua/inervaçãoRESUMO
Force measurements on and within single macromolecular complexes utilizing techniques such as atomic force microscopy, optical trapping, flexible glass fibers, and magnetic beads provide a rich source of quantitative data on biomolecular processes. Stochastic thermal fluctuations, an undesirable source of noise in macroscopic biochemical experiments, are an essential element of these sensitive and novel experiments. With the proper analysis, a great deal of information can be gleaned from measurements of these fluctuations. A quantitative framework for analyzing such measurements, based on Kramers' theory of molecular dissociation, is developed. The analysis reveals the kinetic origin and stochastic nature of the measurements. This framework is presented in the context of protein-ligand separation with the atomic force microscope.
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We report a man with a slowly progressive bulbar palsy 14 years after radiation therapy for nasopharyngeal carcinoma. Electromyography demonstrated prominent myokymic and neuromyotonic discharges in muscles innervated by the lower cranial nerves. Late effects of radiation therapy can occur in the cranial nerve musculature that are similar to well-recognized syndromes affecting the brachial plexus and spinal cord.
Assuntos
Paralisia Bulbar Progressiva/etiologia , Carcinoma/radioterapia , Radioisótopos de Cobalto/efeitos adversos , Traumatismos dos Nervos Cranianos , Neoplasias Nasofaríngeas/radioterapia , Lesões por Radiação , Teleterapia por Radioisótopo/efeitos adversos , Adulto , Anticonvulsivantes/uso terapêutico , Paralisia Bulbar Progressiva/tratamento farmacológico , Paralisia Bulbar Progressiva/fisiopatologia , Caquexia/etiologia , Caquexia/terapia , Radioisótopos de Cobalto/uso terapêutico , Nervos Cranianos/fisiopatologia , Transtornos de Deglutição/etiologia , Transtornos de Deglutição/terapia , Dispneia/etiologia , Eletromiografia , Nutrição Enteral , Humanos , Masculino , Lesões do Pescoço , Músculos do Pescoço/inervação , Pneumonia Aspirativa/etiologia , Lesões por Radiação/diagnóstico , Lesões por Radiação/etiologia , Lesões por Radiação/fisiopatologia , Distúrbios da Fala/etiologia , Fatores de TempoRESUMO
Sodium diethyldithiocarbamate (DDTC) is an investigational modulator of the toxicity produced by cisplatin. The pharmacokinetics of DDTC were evaluated after administration of 200 mg/m2/hr (n = 8) and 400 mg/m2/hr (n = 7) DDTC as 4-hour intravenous infusions to normal male healthy volunteers. Diethyldithiocarbamate concentration at steady-state (Cpss) increased disproportionally from 27.0 +/- 7.6 microM for the low dose to 74.8 +/- 19.3 microM for the high dose, whereas total body clearance decreased from 23.83 +/- 8.23 mL/min/kg for the low dose to 15.48 +/- 2.72 mL/min/kg for the high dose (P < 0.05). However, the volume of distribution in the terminal phase remained unchanged. Diethyldithiocarbamate terminal elimination half-life (t1/2 beta) increased from 3.74 +/- 1.10 minutes for the low dose to 6.08 +/- 1.07 minutes for the high dose (P < 0.005). The data were then fitted using a one-compartment open model with zero-order infusion and Michaelis-Menten elimination kinetics. The Km for DDTC was estimated to be 124.3 +/- 19.9 microM, whereas the Vm was estimated to be 3.67 +/- 1.15 mumol/min/kg. However, DDTC t1/2 beta was independent of DDTC concentrations, suggesting that the nonlinearity in DDTC kinetics does not exactly follow Michaelis-Menten elimination kinetics. Thus, DDTC pharmacokinetics are dose dependent and may not be concentration dependent. Clinically, DDTC Cpss will increase nonlinearly with an increase in dose.