Assuntos
Apendicite/diagnóstico por imagem , Apêndice , Coristoma/diagnóstico por imagem , Doenças do Prematuro/diagnóstico por imagem , Hepatopatias/diagnóstico por imagem , Apendicectomia , Apêndice/patologia , Coristoma/patologia , Coristoma/cirurgia , Diagnóstico Diferencial , Humanos , Recém-Nascido , Doenças do Prematuro/patologia , Doenças do Prematuro/cirurgia , Hepatopatias/patologia , Hepatopatias/cirurgia , Masculino , Necrose , UltrassonografiaRESUMO
Neonatal appendicitis is an uncommon disease characterized by high mortality and morbidity. We report the case of a full-term newborn with infectious syndrome. The nonresponse to antibiotic therapy despite good clinical status led to a search for an etiology. Appendicitis with focal peritonitis was discovered on sonography. In each newborn, presenting with unexplained febrile inflammatory syndrome with or without gastrointestinal symptoms, a routine abdominal sonography should be performed to search for intraperitoneal sepsis, including appendicitis.
Assuntos
Apendicite/diagnóstico , Proteína C-Reativa/análise , Humanos , Recém-Nascido , Masculino , Peritonite/diagnósticoAssuntos
Etnicidade/genética , Frequência do Gene , Genes MHC da Classe II , Genes MHC Classe I , Antígenos de Histocompatibilidade Classe I/genética , Epitopos/genética , Variação Genética , Antígenos HLA-A/genética , Antígenos HLA-B/genética , Antígenos HLA-DQ/genética , Antígenos HLA-DR/genética , Humanos , Líbano , Linfócitos T/imunologia , Imunologia de TransplantesRESUMO
Hemodialysis patients are at high risk of developing HCV infection. Reports from various countries have shown a prevalence of 12-29% among this group. The present study aimed at assessing the utility of HCV antibodies and HCV-RNA detection in the diagnosis of HCV in Lebanese hemodialysis patients. One hundred and eight hemodialysis patients from various hospitals in Lebanon were assayed for the presence of anti-HCV antibodies by ELISA and LIA, and for the presence of HCV-RNA by RT-PCR of the 5' Non-coding region (5' NCR). Specificity of the amplicons was confirmed by Southern hybridization. Seventeen out of 108 patients were reactive in ELISA and positive in the Line Immunoassay (LIA). Eleven out of the 17 were positive by RT-PCR. Three out of 29 patients nonreactive in ELISA were positive by RT-PCR. Our results indicate that hemodialysis patients in this study may be grouped into 4 categories. These include (1) patients with viremia and no immune response, (2) patients with no viremia and with an immune response, (3) patients with both viremia and immune response and (4) patients with no viremia and no immune response. The first 3 categories may reflect the different phases of HCV infection and imply that detection of both anti-HCV antibodies and HCV-RNA are needed for the establishment of adequate diagnosis. In addition, data collected from patients implicated in this study show that infection by HCV may be dialysis machine-related, rather than transfusion-related. However, cross-contamination unrelated to machines may also occurs.
Assuntos
Hepacivirus/genética , Anticorpos Anti-Hepatite C/análise , RNA Viral/análise , Diálise Renal , Southern Blotting , Infecção Hospitalar/virologia , Ensaio de Imunoadsorção Enzimática , Hepacivirus/imunologia , Hepatite C/diagnóstico , Hepatite C/etiologia , Hepatite C/imunologia , Humanos , Imunoensaio , Líbano , Hibridização de Ácido Nucleico , Reação em Cadeia da Polimerase , Prevalência , RNA Viral/genética , Diálise Renal/efeitos adversos , Diálise Renal/instrumentação , Fatores de Risco , Fatores de Tempo , Transcrição Gênica , Reação Transfusional , Viremia/diagnósticoRESUMO
We have genotyped the 5' noncoding region of hepatitis C virus in Lebanese hemodialysis patients by reverse transcription-PCR and restriction fragment length polymorphism analysis. Of 50 patients, 15 had the expected 268-bp amplicon by reverse transcription-PCR. Specificity of the amplicons was confirmed by Southern hybridization. Restriction analysis of the amplicons showed the pattern for genotype 4 (common in the Middle East).
Assuntos
DNA Viral/análise , Hepacivirus/genética , Genoma Viral , Hepacivirus/isolamento & purificação , Humanos , Líbano , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de RestriçãoRESUMO
Peroperative echography and cholangiography provide similar information during the exploration of the biliary tree for conventional surgery for gall stones. Peroperative cholangiography is more difficult to perform via the coeolioscope with a failure rate of 10% in the most skillful teams. We investigated the technical feasibility of peroperative echography during coeliosurgery for gall stones. The technique was performed in 13 patients using a pulsed Doppler 7.5 MHz scan laparoscope. The entire biliary tree was successfully imaged in 12 patients and the three structures of the hepatic pedicle were identified in all cases. The exploration took less than 10 minutes and there was no morbidity. One gall stone was discovered and endoscopic sphincterotomy was performed immediately. None of the patients presented signs suggesting residual lithiasis with a mean follow-up of 2 months. This preliminary study suggests that the technique is very favourably feasible and may play an important role in the exploration of the bile tract during coelioscopic cholecystectomy.
