Cadmium toxicity promotes hormonal imbalance and induces the expression of genes involved in systemic resistances in barley.

Cadmium (Cd) is a widely distributed pollutant that adversely affects plants' metabolism and productivity. Phytohormones play a vital role in the acclimation of plants to metal stress. On the other hand, phytohormones trigger systemic resistances, including systemic acquired resistance (SAR) and induced systemic resistance (ISR), in plants in response to biotic interactions. The present study aimed to investigate the possible induction of SAR and ISR pathways in relation to the hormonal alteration of barley seedlings in response to Cd stress. Barley seedlings were exposed to 1.5 mg g-1 Cd in the soil for three days. The nutrient content, oxidative status, phytohormones profile, and expression of genes involved in SAR and ISR pathways of barley seedlings were examined. Cd accumulation resulted in a reduction in the nutrient content of barley seedlings. The specific activity of superoxide dismutase and the hydrogen peroxide content significantly increased in response to Cd toxicity. Abscisic acid, jasmonic acid, and ethylene content increased under Cd exposure. Cd treatment resulted in the upregulation of NPR1, PR3, and PR13 genes in SAR pathways. The transcripts of PAL1 and LOX2.2 genes in the ISR pathway were also significantly increased in response to Cd treatment. These findings suggest that hormonal-activated systemic resistances are involved in the response of barley to Cd stress.

Cyanobacterial elicitor enhances the biomass of Mentha piperita L. and improves the production of high-value rosmarinic acid under in vitro culture of apical meristem.

BACKGROUND: Rosmarinic acid (RA), like other phenolic compounds, is sources of antioxidants and anti-inflammatory agents in medicinal plants. In vitro culture of plants can improve the medicinal plants' metabolite profile and phenolic compound quantity. To date, various methods have been proposed to increase this medicinal metabolite in plants, among which the use of bioelicitors can be mentioned. In the present study, a native isolate of heterocystous cyanobacteria, Nostoc spongiaeforme var. tenue ISB65, was used to stimulate the production of biomass and content of RA in Mentha piperita L. (peppermint) grown in vitro from apical meristem. Mentha piperita L. explants were inoculated in half strength Murashige and Skoog (1/2 MS) medium containing cyanobacterial lysate (CL). After 50 days of culturing, the growth indices, the content of photosynthetic pigments, and RA in control and treated plants were measured. RESULTS: CL inoculation resulted in a significant enhancement in the vegetative growth indices of peppermint, including root and shoot length, plant biomass and leaf number. The content of photosynthetic pigments also increased in cyanobacteria-treated plants. Inoculation with CL increased the RA content by 2.3-fold, meaning that the plants treated with CL had the highest RA content (7.68 mg. g- 1 dry weight) compared to the control (3.42 mg. g- 1 dry weight). Additionally, HPLC analysis revealed the presence of several auxins in CL. CONCLUSIONS: The presence of auxins and the chemical content of CL such as K+ and Ca2+, as regulators of metabolic pathways and molecular activities of cells, may be responsible for the enhanced growth and phenolic compounds of plants under tissue culture conditions. An improvement in RA content in the tissue culture of medicinal plants treated with CL was reported for the first time in this investigation.

Growth enhancement, metabolic profile improvement, and DXR and TPS2 gene expression changes in Thymus vulgaris L. by cyanobacterial inoculation.

BACKGROUND: In recent decades cyanobacterial species have attracted research attention as potential sources of new biostimulants. In this study, the biostimulant effects of five cyanobacterial suspensions on the growth and essential oil composition of Thymus vulgaris L. were evaluated. The expression of key genes involved in the biosynthesis of thymol and carvacrol, such as DXR and TPS2, were investigated. RESULTS: A pot culture experiment revealed that cyanobacterial application significantly improved T. vulgaris L. growth indices, including plant height, dry and fresh weight, leaf and flower number, leaf area, and photosynthetic pigment content. Total phenol and flavonoid content in inoculated plants also showed a significant increase compared with the control. Anabaena torulosa ISB213 inoculation significantly increased root and shoot biomass by about 65.38% and 92.98% compared with the control, respectively. Nostoc calcicola ISB215 inoculation resulted in the highest amount of essential oil accumulation (18.08 ± 0.62) in T. vulgaris leaves, by about 72.19% compared with the control (10.5 ± 0.50%). Interestingly, the amount of limonene in the Nostoc ellipsosporum ISB217 treatment (1.67%) increased significantly compared with the control and other treatments. The highest expression rates of DXR and TPS2 genes were observed in the treatment of N. ellipsosporum ISB217, with 5.92-fold and 5.22-fold increases over the control, respectively. CONCLUSION: This research revealed the potential of the cyanobacteria that were studied as promising biostimulants to increase the production of biomass and secondary metabolites of T. vulgaris L., which could be a suitable alternative to chemical fertilizers. © 2024 Society of Chemical Industry.

Integrated approaches for heavy metal-contaminated soil remediation: harnessing the potential of Paulownia elongata S. Y. Hu, Oscillatoria sp., arbuscular mycorrhizal fungi (Glomus mosseae and Glomus intraradices), and iron nanoparticles.

In recent years, researchers have extensively investigated the remediation of heavy metal-contaminated soil using plants, microorganisms, and iron nanoparticles. The objective of this study was to investigate and compare the individual and simultaneous effects of Paulownia elongata S. Y. Hu, cyanobacteria (Oscillatoria sp.), arbuscular mycorrhizal fungi (AMF) including Glomus mosseae and Glomus intraradices, and zero-valent iron nanoparticles (nZVI) on the remediation of heavy metal-contaminated soil containing chromium (Cr VI and Cr III) and nickel (Ni). The study found significant variations in parameters such as pH (acidity), electrical conductivity (EC), nitrogen (N), phosphorus (P), potassium (K), and organic carbon (OC) among different treatments. The addition of cyanobacteria, AMF, and nZVI influenced these properties, resulting in both increases and decreases compared to the control treatment. The treatment involving a combination of cyanobacteria, AMF, and nZVI (CCAN25) exhibited the highest increase in growth parameters, such as total dry mass, root length, stem diameter, and leaf area, while other treatments showed varied effects on plant growth. Moreover, the CCAN25 treatment demonstrated the highest increase in chlorophyll a, chlorophyll b, and carotenoid levels, whereas other treatments displayed reductions in these pigments compared to the control. Moderate phytoaccumulation of Cr and Ni in P. elongata samples across all treatments was observed, as indicated by the bioconcentration factor and bioaccumulation coefficient values being less than 1.0 for both metals. The findings provide insights into the potential application of these treatments for soil remediation and plant growth enhancement in contaminated environments.

Enhancement of Essential Oil Production and Expression of Some Menthol Biosynthesis-Related Genes in Mentha piperita Using Cyanobacteria.

Background: Mentha piperita L. is one of the most important aromatic crops and is cultivated worldwide for essential oils (EOs). Objectives: The aim of the present study was to investigate the potential of two cyanobacteria, Anabaena vaginicola ISB42 and Nostoc spongiaeforme var. tenue ISB65, as biological-elicitors to improve the growth and essential oil production of M. piperita. Materials and Methods: In this experiment, inoculation of M. piperita with cyanobacteria was performed by adding 1% cyanobacterial suspension to the soil of treated pots on the first time of planting and every 20 days thereafter. The experiment was performed in a randomized complete block design in an experimental greenhouse condition. After 90 days planting, the vegetative growth factors, the content of photosynthetic pigments, as well as the quantity and quality of EOs of treated and control plants were evaluated. Also, quantitative changes in the expression of some menthol biosynthesis-related genes were investigated. Results: Cyanobacterial application led to significant increases in M. piperita growth indices including root and shoot biomass, leaf number, leaf area, node number and ramification, as well as photosynthetic pigments content. The statistical analysis showed a 41-75 % increase in some of these growth indices, especially in Nostoc-treated plants. A. vaginicola and N. spongiaeforme var. tenue inoculation led to a 13% and 25% increase in the EOs content of M. piperita, respectively. The EOs components were also affected by cyanobacterial treatments. According to the statistical analysis, Nostoc-treated plants showed the highest amount of (-)-menthone and (-)-limonene, with a 2.36 and 1.87-fold increase compared to the control. A. vaginicola and N. spongiaeforme var. tenue inoculation also led to 40% and 98% increase in transcript level of (-)-limonene synthase gene, respectively. The expression of the (-)-menthone reductase gene, was also increased by 65% and 55% in response to A. vaginicola and N. spongiaeforme var. tenue application, respectively. Conclusions: Our data demonstrated that in addition to growth enhancement, these two heterocystous cyanobacteria improved the quantity and quality of EOs by up-regulating the key genes involved in the menthol biosynthetic pathway. Based on our results, these cyanobacteria can be considered valuable candidates in the formulation of low-cost and environmentally friendly biofertilizers in sustainable peppermint production.

Satellite-Based Monitoring of the Algal Communities of Aras Dam Reservoir: Meteorological Dependence Analysis and the Footprint of COVID-19 Pandemic Lockdown on the Eutrophication Status.

Aras Dam Lake is a strategic aquatic ecosystem in Iran and there are reports of toxic phytoplankton blooms in this reservoir. This study was performed to determine the effect of meteorological variables on the formation and expansion of toxic phytoplankton communities in Aras dam reservoir. The data of this project have been obtained using field studies and satellite data (MODIS and Sentinel-2). Sampling to determine the composition of phytoplankton communities in the area was carried out seasonally in two time periods from 2003 to 2014, and environmental assessments were also performed based on meteorological and satellite data over an 18-year period (2003-2020). The Chlorophyll-a content was obtained from MODIS and correlated with meteorological data. The statistical analysis showed that the highest coefficient of determination is related to the correlation of chlorophyll-a and Evaporation (R 2 = 0.86). Also, the relative root mean square error is equal to 18%, 18.1% and 21.2% for the chlorophyll-a -SST, chlorophyll-a -wind and chlorophyll-a -Evaporation relations, respectively. Moreover, in a supplementary study, correlation between the chlorophyll-a content with selected meteorological variables including evaporation, wind speed and water surface temperature were investigated seasonally. The results showed that the trend of changes in chlorophyll-a content with three considered variables are parabolic functions and chlorophyll-a -Evp (R 2 = 0.86, MAPE = 15.2%) model indicates better performance. The results also showed that the eutrophication rate of the reservoir during lockdown period increased in comparison with the same time at pre-pandemic period, which can be related to increase of incoming waste loads in this reservoir.

226Ra, 238U and Cd adsorption kinetics and binding capacity of two cyanobacterial strains isolated from highly radioactive springs and optimal conditions for maximal removal effects in contaminated water.

Biomass-based decontamination methods are among the most interesting water treatment techniques. In this study, 2 cyanobacterial strains, Nostoc punctiforme A.S/S4 and Chroococcidiopsis thermalis S.M/S9, isolated from hot springs containing high concentrations of radium (226Ra), were studied to be associated with removal of radionuclides (238U and 226Ra) and heavy metal cadmium (Cd) from aqueous solutions. The adsorption equilibrium data was described by Langmuir and Freundlich isotherm models. Kinetic studies indicated that the sorption of 3 metals followed pseudo-second-order kinetics. Effects of biomass concentration, pH, contact time, and initial metal concentration on adsorption were also investigated. Fourier-transform infrared spectroscopy revealed active binding sites on the cyanobacterial biomass. The obtained maximum biosorption capacities were 630 mg g-1 and 37 kBq g-1 for 238U and 226Ra for N. punctiforme and 730 mg g-1 and 55 kBq g-1 for C. thermalis. These 2 strains showed maximum binding capacity 160 and 225 mg g-1, respectively for Cd adsorption. These results suggest that radioactivity resistant cyanobacteria could be employed as an efficient adsorbent for decontamination of multi-component, radioactive and industrial wastewater.

Analysis of Anabaena vaginicola and Nostoc calcicola from Northern Iran, as rich sources of major carotenoids.

Four major carotenoids of high nutritional significance, including ß-carotene, lycopene, lutein and zeaxanthin were determined in three isolates of heterocystous cyanobacteria, belonging to the genera Anabaena and Nostoc, isolated from Iranian terrestrial and aquatic ecosystems, for the first time. The ultrasonically extracted carotenoids were identified and quantified by a rapid and sensitive isocratic HPLC method and identification was further confirmed by spiking authentic standards and the pattern of the UV-Vis spectra obtained from photo-diode array detector. The results showed that these isolates contain large amounts of four major carotenoids, especially lycopene (up to 24,570 µg/g dry weight, DW) which appears to be the highest reported amount until present; and ß-carotene (up to 8133 µg/g DW) which is comparable with the best natural sources of ß-carotene. Meanwhile, they are rich in the cis-isomers of lycopene and ß-carotene which is important in their bioavailability and health benefits.