Qualitative and quantitative simulation of best management practices (BMPs) for contaminated megasite remediation using the SiteWise™ tool.

Remediation activities, particularly in megasites, may induce substantial secondary environmental impacts that must be addressed for green and sustainable remediation (GSR) practices. Only limited studies are available quantitatively assessing the environmental footprint and environmental benefits of implementing Best Management Practices (BMPs) in megasite remediation. This study used the SiteWise™ tool, a quantitative environmental footprint assessment for scenario simulation and benefit quantification of BMPs, on a contaminated megasite in Hebei Province, China. We observed a considerable environmental footprint and energy from the remediation. Taking the final implementation alternative (Alt 1) as an example, which is characterized by combining multiple remediation techniques, the greenhouse gas (GHG) emissions reached 113,474 t, the energy used was 2,082,841 million metric British thermal units (MMBTU), and other air pollutant emissions (NOx, SOx, and PM10) amounted to 856 t. Further BMP analyses highlighted the benefits of substituting the conventional solidification/stabilization agent with willow woodchip-based biochar, which could reduce GHG emissions by 50,806 t and energy used by 926,648 MMBTU. The overall environmental benefits of implementing all applicable BMPs in the remediation were significant, with a 66.85%, 50.15%, and 56.05% reduction in GHG emissions, energy used, and other air pollutants, respectively. Our study provides insights into quantifying the environmental footprint and exploring emission reduction pathways for contaminated megasite remediation. It also offers a feasible path for quantifying the environmental benefits of BMPs, promoting the development of GSR of contaminated sites.

Construction of a bacteriophage-derived vector with potential applications in targeted drug delivery and cell imaging.

There is a strong relationship between the dysregulation of epidermal growth factor receptor (EGFR) and the development of epithelial-derived cancers. Therefore, EGFR has usually been considered the desired target for gene therapy. Here, we propose an approach for targeting EGFR-expressing cells by phage particles capable of displaying EGF and GFP as tumor-targeting and reporting elements, respectively. For this purpose, the superfolder GFP-EGF (sfGFP-EGF) coding sequence was inserted at the N-terminus of the pIII gene in the pIT2 phagemid. The capability of the constructed phage to recognize EGFR-overexpressing cells was monitored by fluorescence microscopy, fluorescence-activated cell sorting (FACS), and cell-based ELISA experiments. FACS analysis showed a significant shift in the mean fluorescence intensity (MFI) of the cells treated with phage displaying sfGFP-EGF compared to phage displaying only sfGFP. The binding of phage displaying sfGFP-EGF to A-431 cells, monitored by fluorescence microscopy, indicated the formation of the sfGFP-EGF-EGFR complex on the surface of the treated cells. Cell-based ELISA experiments showed that phages displaying either EGF or sfGFP-EGF can specifically bind EGFR-expressing cells. The vector constructed in the current study has the potential to be engineered for gene delivery purposes as well as cell-based imaging for tumor detection.

Horticultural Practices in Early Spring to Mitigate the Adverse Effect of Low Temperature on Fruit Set in 'Lapins' Sweet Cherry.

Yield of sweet cherry (Prunus avium L.) is determined by fruit set, a developmental stage sensitive to variable spring environmental conditions. To sustain fruit production and enhance crop climate resilience, it is important to understand the impacts of abiotic stresses and the effectiveness of horticultural mitigations in the spring on the critical developmental processes during fruit set. In this study, flowering phenology, pistil browning and percent fruit set of 'Lapins' were monitored at five sites of different elevation and frost risk in the Okanagan Valley, British Columbia, Canada, in 2019 and 2022. At Site 1 in Summerland Research and Development Centre ("SuRDC1"), where a 'Lapins' on Krymsk 5 planting was located in a frost pocket where the crops were exposed to high risk of cold damage in the spring, a series of experiments were conducted to investigate the floral organ viability and percent fruit set under low temperatures, and under the effects of four spring horticultural mitigation measures. Installation of polyethylene sleeves and FAME spray (fatty acid methyl esters-based plant growth regulator, WAIKEN, SST Australia) were implemented in 2019; boric acid spray and postponed irrigation were tested in 2022. Low fruit set at SuRDC1 in both years was associated with severe pistil browning after night temperature dropped below -4 °C in late April. In 2019, the semi-enclosure of polyethylene sleeves led to an increase in the surface temperature (Tsurfae) of floral buds by 2-4 °C, which prolonged the stage of first bloom, delayed petal fall and prevented frost damage on pistils, but led to the decrease in percent fruit set by 77%, due to ovule abortion or cessation of fruitlet development. The early and late sprays of FAME had no significant influence on either abundance of germinated pollen tubes or percent fruit set; however, the potential of late spray in improving pollen abundance and reducing pistil browning requires further investigation. In 2022, the spray of 0.01% boric acid solution led to a decrease in fruit set by 6.95%. Six-week postponement of irrigation starting from full bloom decreased soil moisture, but increased soil temperature and improved fruit set by 7.61%. The results improved our understanding about the damages of adverse spring air temperatures on pistils and ovules, and suggested the potential of irrigation adjustment in regulating soil moisture and temperature and improving fruit set in the cool and moist spring.

The Challenges of Caring for an Adult Child with Schizophrenia in the Family: An Analysis of the Lived Experiences of Older Parents.

The present study aimed to investigate the lived experiences of older parents regarding the challenges in caring for adult children with schizophrenia. The current study was conducted on 16 parental (mother or father) caregivers of adult children with schizophrenia using a descriptive phenomenological qualitative approach and Colaizzi's seven-step method. This study follows the Qualitative Research Checklist (COREQ). The findings showed that the parent caregivers' experiences could be classified into two main themes: "burden of care" and "Negative attitude and inefficient performance." The former consisted of three sub-themes including "disrupted social and family interactions," "helplessness and inefficient support," and "challenges of the healthcare system," while the latter had two sub-themes including "Negative attitude and inefficient performance of the caregivers" and "Negative attitude and inefficient performance of families and society." Older parents have to tolerate a significant burden of care due to their age and physical conditions. Improving the knowledge of specialists, the government's redoubled efforts in multifaceted support for patients and caregivers, creating an integrated team of specialists, and accepting and improving public attitudes against stigma and obvious discrimination in society as important priorities in improving the condition of caregivers and patients with schizophrenia were considered.

Physical activity and psychological adjustment among retirees: a systematic review.

BACKGROUND: Health-related behaviors may change after retirement and induce changes in the mental health. This systematic review aimed to investigate the nature of changes in physical activity and leisure activities, as well as the relationship between physical activity, leisure, and psychological adjustment among retirees. METHODS: Search of papers was done in three electronic databases of PubMed, ISI Web of Science, and Scopus without constraints on time, geographical regions, or languages in February 2022. The papers that had examined the relationship between physical activity and adjustment among retirees using observational design (cross-sectional or longitudinal) were included in the present study. To evaluate the methodological quality of cross-sectional studies, Joanna Briggs Institute (JBI) Critical Appraisal Checklist, and for longitudinal studies, Newcastle-Ottawa Scale (NOS) were used. RESULTS: The search results identified 1458 records. Twenty-six papers were included in this review based on the inclusion and exclusion criteria. The findings of most of these studies indicated a significant positive correlation between physical activity, leisure, and psychological adjustment in retirees. Retirees were mostly engaged in passive leisure activities such as reading, watching TV and movies, and less engaged in physical activities, sport, or physical exercise. General organized assessment of the total physical activity among retirees was not possible. CONCLUSIONS: Based on the study findings, it can be stated that there is a positive correlation between physical activity, leisure, and the retirees' adjustment. Usage of the same and valid measurement method specific to old age and retirement period can be useful in more precise assessment of physical activity and its association with adjustment among retirees.

Comparative Study of Fentanyl vs Dexmedetomidine as Adjuvants to Intrathecal Bupivacaine in Cesarean Section: A Randomized, Double-Blind Clinical Trial.

PURPOSE: Effective postoperative analgesia is essential in cesarean section. This study aimed to compare postoperative analgesia and hemodynamic changes after intrathecal use of fentanyl or dexmedetomidine combined with bupivacaine. PATIENTS AND METHODS: This study involved 110 pregnant women with ASA I and II and gestational age ≥37 weeks who were candidates for elective cesarean section. They were randomly divided into two groups of 55; Group B-D received 10 mg bupivacaine (0.5%) + 5 µg dexmedetomidine and Group B-F received 10 mg bupivacaine (0.5%) + 25 µg fentanyl, intrathecally. The onset of block, duration of analgesia, the score of pain intensity, hemodynamic changes, Apgar scores, and any adverse events were evaluated. P-value <0.05 was considered statistically significant. RESULTS: Patients in two groups were similar in terms of demographic characteristics and ASA classification. Duration of analgesia in the B-D group was significantly longer than B-F group (428.64±73.39 vs 273.18±61.91 min; P<0.001). The score of pain intensity during recovery time in the B-D group was significantly lower than that of B-F group (0.33±0.84 vs 0.51±0.57 min; P=0.004). The onset of block was also faster in the B-D group than B-F group (98.27±35.95 vs 110.45±37.69 seconds; P=0.036). The two groups did not show significant differences in hemodynamic changes and other variables (P>0.05). CONCLUSION: Compared with fentanyl, it seems that adding 5 µg dexmedetomidine to bupivacaine has a better effect on postoperative pain management in cesarean section under spinal anesthesia.

Spatial distribution of soil phosphorous fractions following 1-year farrowing sows in an outdoor hog-rearing farm in Eastern Canada.

Outdoor hog-rearing operations are of interest for both producers and consumers due to high product quality, animal welfare status, and low input and potential environmental risks. However, hog manure is rich in phosphorus (P), an environmentally sensitive nutrient, and distribution of different P fractions down the soil profile in these production systems is not well understood. The objective of this study was to determine the spatial variability of soil P in different soil depth intervals following 1-year outdoor farrowing sows in a 0.5-ha paddock in cold-temperate climate of Eastern Canada. Soil samples were collected with 0-15, 15-30, and > 30 cm depth intervals (up to 60 cm or the depth that sampling was possible) in grazing/rooting, feeding, wallow, and farrowing hut areas. Soil samples were analyzed for Olsen P (Pol), organic P (PO), and total P (PT). Areas with more frequent presence of hogs showed 45-80% greater Pol concentrations, and movement of soluble PO down the soil profile was higher in these areas compared with the grazing/rooting area. The PO formed 80% of PT throughout the paddock, and the spatial distribution of PO was similar to PT in all soil depth intervals but different from Pol. Results also showed that PO concentrations in the paddock decreased at > 30 cm depth except for the feeding area. Findings of this study reveal that farrowing sow cycle in an outdoor hog-rearing farm setting can result in hot spots of P, which enhance the risk of environmental pollution.

Characterization of Novel Fragment Antibodies Against TNF-alpha Isolated Using Phage Display Technique.

Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine which plays crucial roles in pathogenesis of inflammatory diseases. The current study aimed to investigate the binding abilities of I44 and I49 domain antibodies to TNF-α. The dAbs were expressed in bacterial expression system and purified by affinity chromatography using Ni-sepharose column. The expression and purity of the proteins were evaluated using western blotting and SDS-PAGE techniques, respectively. ELISA experiment showed that I44 and I49 dAbs bind to TNF-α with the binding constants (Kd) of 5.18 ± 1.41 and 2.42 ± 0.55 µM, respectively. The inhibitory effect of dAbs on TNF-α biological effect was determined in MTT assay in which I44 and I49 prevented TNF-α cell cytotoxicity with IC50 values of 6.61 and 3.64 µM, respectively. The identified anti-TNF-α dAbs could bind to and inhibit TNF-α activity. The dAbs activities can be attributed to their ability to establish hydrogen bonds as well as hydrophobic contacts with TNF-α. The results of the current study can pave the way for further structural studies in order to introduce new more potent anti-TNF-α antibodies.

An Alignment-Independent 3D-QSAR Study of FGFR2 Tyrosine Kinase Inhibitors.

Purpose: Receptor tyrosine kinase (RTK) inhibitors are widely used pharmaceuticals in cancer therapy. Fibroblast growth factor receptors (FGFRs) are members of RTK superfamily which are highly expressed on the surface of carcinoma associate fibroblasts (CAFs). The involvement of FGFRs in different types of cancer makes them promising target in cancer therapy and hence, the identification of novel FGFR inhibitors is of great interest. In the current study we aimed to develop an alignment independent three dimensional quantitative structure-activity relationship (3D-QSAR) model for a set of 26 FGFR2 kinase inhibitors allowing the prediction of activity and identification of important structural features for these inhibitors. Methods: Pentacle software was used to calculate grid independent descriptors (GRIND) for the active conformers generated by docking followed by the selection of significant variables using fractional factorial design (FFD). The partial least squares (PLS) model generated based on the remaining descriptors was assessed by internal and external validation methods. Results: Six variables were identified as the most important probes-interacting descriptors with high impact on the biological activity of the compounds. Internal and external validations were lead to good statistical parameters (r2 values of 0.93 and 0.665, respectively). Conclusion: The results showed that the model has good predictive power and may be used for designing novel FGFR2 inhibitors.

Folliculotropic Mycosis Fungoides: Clinical and Histologic Features in Five Patients.

BACKGROUND: Alopecia can be a manifestation of mycosis fungoides (MF); however, the prevalence is unknown. AIMS: We sought to describe the clinicopathologic presentation of alopecia in patients with diagnosis of MF. METHODS: A retrospective analysis of patients with biopsy-proven MF, who were evaluated at our cancer center from 2002 to 2012, was performed to identify patients with alopecia. RESULTS: Five patients with alopecia were identified from reviewing of 157 patients with MF. The male:female ratio was 3:2, and the mean age of patients was 42.8 years. Two of these patients showed patchy hair loss on scalp which was clinically identical to alopecia areata. In remaining three patients, hair loss was seen in areas of MF lesions, and epidermal changes consisted of patch- and plaque-type lesions of MF, tumors, and follicular lesions (follicular MF) were also present. In two of these patients, lymphadenopathy without any visceral involvement was detected. CONCLUSIONS: Alopecia was observed in 5 (3.18%) patients with MF, which makes it a rare finding, which included alopecia areata-like patchy loss in 2 and alopecia within MF lesions in 3.

Explosive photodissociation of methane induced by ultrafast intense laser.

A new type of molecular fragmentation induced by femtosecond intense laser at the intensity of 2 x 10(14) W/cm2 is reported. For the parent molecule of methane, ethylene, n-butane, and 1-butene, fluorescence from H (n = 3-->2), CH (A 2Delta, B 2Sigma-, and C 2Sigma+-->X 2Pi), or C2 (d 3Pi g-->a 3Pi u) is observed in the spectrum. It shows that the fragmentation is a universal property of neutral molecule in the intense laser field. Unlike breaking only one or two chemical bonds in conventional UV photodissociation, the fragmentation caused by the intense laser undergoes vigorous changes, breaking most of the bonds in the molecule, like an explosion. The fragments are neutral species and cannot be produced through Coulomb explosion of multiply charged ion. The laser power dependence of CH (A-->X) emission of methane on a log-log scale has a slope of 10 +/- 1. The fragmentation is thus explained as multiple channel dissociation of the superexcited state of parent molecule, which is created by multiphoton excitation.