RESUMO
The paper deals with the mathematical processing of enzyme immunoassay (EIA) data. The paper comprises two parts. Part 1 contains a brief comparative account of the basic ways of presenting experimental data and constructing a standard curve, as well as practical guidelines for choosing the mathematical methods for calculation of concentrations. The recommendations proposed in Part II are based on the comparative study of photometric equipment and software that is of common use in EIA diagnosis at the Russian clinical laboratories. The experiment imitates competitive analysis illustrates a relationship of the obtained clinical result to the choice of either mode of constructing the standard graph on the basis of the optic densities of calibration samples. The findings indicate that an incorrectly chosen method for data processing may make both a false rejection of the whole analysis on the basis of the erroneous calculation of the concentration of a test agent in the control serum and to underdiagnosis on the basis of the erroneous calculation of the concentration of a test agent in a patient's sample near the point of taking a medical decision.