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1.
Biochem Biophys Rep ; 20: 100692, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31650041

RESUMO

Dextransuccrase (E.C 2.4.1.5) is a key enzyme in S. mutans for the metabolism of sucrose which helps in the adherence and accumulation of bacteria on tooth surface leading to the formation of dental caries. Dextransuccrase resembles in its catalytic properties with the brush boarder sucrase and exhibits pH dependent inhibitory and stimulatory effects in response to Na+. In this communication we studied the effect of monovalent cations on the activity of dextransuccrase from S. mutans. The percentage inhibition of dextransuccrase was 65% at 0.5 mM NaCl which enhanced to 90% at 20 mM sodium concentration. However there was no effect on dextransucrase activity in presence of other monovalent cations (Rb+, Cs+, and K+) tested. Enzyme activity was enhanced 20-24% in acidic pH but was strongly inhibited (59-89%) around neutral and alkaline pH by 0.5-2.0 mM sodium chloride. Upon dialysis, 86% of enzyme activity was restored to control values. There was no effect of 2 mM NaCl on glucosyltransferase activity of the enzyme. Kinetic studies revealed that enzyme showed biphasic effects in response to Na+ ions. At acidic pH the enzyme exhibited mixed type of activation affecting both Vmax and Km, while in alkaline pH, the enzyme showed V- type effect reducing Vmax by 74% without affecting Km. The effects of sodium ions on dextransuccrase activity were specific, thus it can be useful to block its catalytic activity, and reducing the cariogenic potential of S. mutans.

2.
FEBS Lett ; 591(13): 1993-2002, 2017 07.
Artigo em Inglês | MEDLINE | ID: mdl-28486745

RESUMO

We have identified in yeast a connection between two master regulators of cell growth: a biochemical connection involving the TORC1 protein kinase (which activates protein synthesis, nutrient uptake, and anabolism) and a biophysical connection involving the plasma membrane proton-pumping H+ -ATPase Pma1 (which drives nutrient and K+ uptake and regulates pH homeostasis). Raising the temperature to nonpermissive values in a TOR thermosensitive mutant decreases Pma1 activity. Rapamycin, a TORC1 inhibitor, inhibits Pma1 dependent on its receptor Fpr1 and on the protein phosphatase Sit4, a TORC1 effector. Mutation of either Sit4 or Tco89, a nonessential subunit of TORC1, decreases proton efflux, K+ uptake, intracellular pH, cell growth, and tolerance to weak organic acids. Tco89 does not affect Pma1 activity but activates K+ transport.


Assuntos
Membrana Celular/metabolismo , Homeostase , Complexos Multiproteicos/metabolismo , Potássio/metabolismo , Bombas de Próton/metabolismo , Saccharomyces cerevisiae/citologia , Saccharomyces cerevisiae/metabolismo , Serina-Treonina Quinases TOR/metabolismo , Transporte Biológico , Concentração de Íons de Hidrogênio , Espaço Intracelular/química , Alvo Mecanístico do Complexo 1 de Rapamicina , Mutação , Proteína Fosfatase 2/genética , Proteína Fosfatase 2/metabolismo , ATPases Translocadoras de Prótons/metabolismo , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/metabolismo
3.
Curr Microbiol ; 62(3): 944-9, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-21061124

RESUMO

The aim of this study was to explore the relationship of intracellular glutathione with various oxidative stress markers and the stress protectant marker trehalose. In the first group of yeast cells, diethyl maleate was used for depletion of glutathione. A second group of yeast cells were incubated with amino acids constituting glutathione (GIu, Cys, Gly) to increase glutathione level. Increased level of oxidative stress marker like ROS, protein carbonyl formation and lipid peroxidation and decreased viability in glutathione-depleted cells were observed in the present study. The increased activity of antioxidant enzymes SOD and CAT in the glutathione depleted group suggests the interaction of different antioxidant defence system in Pachysolen tannophilus. Furthermore, the increased levels of trehalose in glutathione-depleted group shows that trehalose acts as a stress reducer in glutathione depleted Pachysolen tannophilus.


Assuntos
Catalase/metabolismo , Glutationa/metabolismo , Estresse Oxidativo , Saccharomycetales/efeitos dos fármacos , Saccharomycetales/fisiologia , Estresse Fisiológico , Superóxido Dismutase/metabolismo , Antioxidantes/metabolismo , Viabilidade Microbiana/efeitos dos fármacos , Trealose/metabolismo , Leveduras
4.
Yeast ; 27(9): 713-25, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20213854

RESUMO

Glucose, in the absence of additional nutrients, induces programmed cell death in yeast. This phenomenon is independent of yeast metacaspase (Mca1/Yca1) and of calcineurin, requires ROS production and it is concomitant with loss of cellular K(+) and vacuolar collapse. K(+) is a key nutrient protecting the cells and this effect depends on the Trk1 uptake system and is associated with reduced ROS production. Mutants with decreased activity of plasma membrane H(+)-ATPase are more tolerant to glucose-induced cell death and exhibit less ROS production. A triple mutant ena1-4 tok1 nha1, devoid of K(+) efflux systems, is more tolerant to both glucose- and H(2)O(2)-induced cell death. We hypothesize that ROS production, activated by glucose and H(+)-ATPase and inhibited by K(+) uptake, triggers leakage of K(+), a process favoured by K(+) efflux systems. Loss of cytosolic K(+) probably causes osmotic lysis of vacuoles. The nature of the ROS-producing system sensitive to K(+) and H(+) transport is unknown.


Assuntos
Morte Celular , Glucose/toxicidade , Peróxido de Hidrogênio/toxicidade , Hidrogênio/metabolismo , Potássio/metabolismo , Saccharomyces cerevisiae/efeitos dos fármacos , Proteínas de Transporte de Cátions/metabolismo , ATPase Trocadora de Hidrogênio-Potássio/metabolismo , Pressão Osmótica , Espécies Reativas de Oxigênio/toxicidade , Saccharomyces cerevisiae/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo
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