Beyond growth: a wellbeing economy for the textile and garment sector.

BACKGROUND/OBJECTIVE: Growth-alternative economic models such as wellbeing economies, steady state and degrowth perspectives have come to prominence as opportunities to foster human health and quality of life without exceeding planetary boundaries. Collectively these perspectives offer an avenue for holistic approaches to addressing planetary and human health, but to implement them will require substantial changes to institutions, governance systems and our general ways of life. Drawing from a literature review, our team identified four attributes of wellbeing economies and applied them to the textile and garment sector - one of the most globalised and complex supply chains. Type of program or service Application of alternative economic systems for planetary and human health. METHODS: We use a case study approach to analyse the global textile and garment industry 1, drawing on previously published literature to identify key attributes of wellbeing economies and demonstrate how they can be applied in practice. RESULTS: We describe four central principles for implementing growth alternative economic models in the fashion industry: i) Establishing limits; ii) Promoting fairness; iii) Developing new and just governance systems; and iv) Promoting new roles for business and systems of exchange. LESSONS LEARNT: Significant societal transformations will be required to achieve growth-alternative economic approaches; however, these approaches offer a real chance for achieving planetary and human health. The textile and garment industry provides a valuable case study to explore these possibilities.

Clinical Characteristics and Etiology of Community-acquired Pneumonia in US Children, 2015-2018.

BACKGROUND: Pneumonia has a major impact on childhood health and health care costs. This study was designed to obtain contemporary information on the clinical characteristics and etiology of community-acquired pneumonia (CAP) in children from both inpatient and outpatient settings in the USA. METHODS: We conducted a prospective, multicenter, observational study of CAP among previously healthy children 2 months to 18 years of age in 6 children's hospitals in Ohio from 2015 to 2018. For pathogen detection, nasopharyngeal swabs were collected from all subjects. Blood and pleural fluid cultures were available per standard of care. RESULTS: We enrolled a convenience sample of 441 patients: 380 hospitalized and 61 outpatients. Tachypnea and radiologic findings of consolidation and pleural effusion were more frequent among inpatients than outpatients. A pathogen was detected in 64.6% of patients: viruses in 55.6%, atypical bacteria in 8.8% and pyogenic bacteria in 4.3%. Eighteen (4.1%) patients had both viruses and bacteria detected. Rhinovirus/enterovirus (RV; 18.6%) and respiratory syncytial virus (RSV; 16.8%) were the viruses most frequently detected, and Mycoplasma pneumoniae (8.2%) and Streptococcus pneumoniae (2.3%) were the most common bacteria. Except for S. pneumoniae, which was identified more frequently in inpatients, there were no significant differences between inpatients and outpatients in the proportions of children with specific pathogens detected. CONCLUSIONS: Rhinovirus/enterovirus and RSV among viruses and M. pneumoniae and S. pneumoniae among bacteria were the most common pathogens detected in children with CAP. Tachypnea and chest radiographs with consolidation and/or pleural effusion were associated with hospitalization.

SapF-mediated heme-iron utilization enhances persistence and coordinates biofilm architecture of Haemophilus.

Non-typeable Haemophilus influenzae (NTHI) is a common commensal bacterium that resides in the human upper respiratory tract of healthy individuals. NTHI is also a known causative agent of multiple diseases including sinusitis, otitis media, as well as exacerbates disease severity of patients with cystic fibrosis and chronic obstructive pulmonary disease. We have previously shown that the Sap transporter mediates resistance to host antimicrobial peptides (AMPs) and import of the iron-containing compound heme. Here, we analyzed the contribution of the Sap structural ATPase protein, SapF, in these essential functions. In contrast to SapD, SapF was dispensable for NTHI survival when exposed to AMPs in vitro. SapF was responsible for heme utilization and recovery of depleted internal heme-iron stores. Further, a loss of SapF resulted in morphological plasticity and enhanced community development and biofilm architecture, suggesting the potential role of heme-iron availability in coordinating the complexity of NTHI biofilm architecture. SapF was required for colonization of the nasopharynx and acute infection of the middle ear, as SapF deficiency correlated with a statistically significant decrease in NTHI persistence in vivo. These data suggest that SapF is required for proper heme utilization which directly impacts NTHI survival. Thus, these studies further support a role for the Sap complex in the transport of multiple substrates and further defines substrate specificity for the two ATPase subunits. Given the multiple essential functions provided by the Sap transporter, this complex could prove to be an effective therapeutic target for the treatment of NTHI diseases.

Pediatric battery-related emergency department visits in the United States, 1990-2009.

OBJECTIVE: To investigate the epidemiology of battery-related emergency department (ED) visits among children <18 years of age in the United States. METHODS: Using a nationally representative sample from the National Electronic Injury Surveillance System, battery-related ED visits in the United States from 1990 to 2009 were analyzed. Four battery exposure routes for patients were determined from diagnosis codes and case narratives: ingestion, mouth exposure, ear canal insertion, and nasal cavity insertion. RESULTS: An estimated 65788 (95% confidence interval: 54498-77078) patients <18 years of age presented to US EDs due to a battery-related exposure during the 20-year study period, averaging 3289 battery-related ED visits annually. The average annual battery-related ED visit rate was 4.6 visits per 100000 children. The number (P < .001) and rate (P = .002) of visits increased significantly during the study period, with substantial increases during the last 8 study years. The mean age was 3.9 years (95% confidence interval: 3.5-4.2), and 60.2% of patients were boys. Battery ingestion accounted for 76.6% of ED visits, followed by nasal cavity insertion (10.2%), mouth exposure (7.5%), and ear canal insertion (5.7%). Button batteries were implicated in 83.8% of patient visits caused by a known battery type. Most children (91.8%) were treated and released from the ED. CONCLUSIONS: This study evaluated battery-related ED visits among US children using a nationally representative sample. Batteries pose an important hazard to children, especially those ≤ 5 years of age. The increasing number and rate of battery-related ED visits among children underscore the need for increased prevention efforts.

Elicitation of epithelial cell-derived immune effectors by outer membrane vesicles of nontypeable Haemophilus influenzae.

Outer membrane vesicles (OMVs) are produced by all Gram-negative microorganisms studied to date. The contributions of OMVs to biological processes are diverse and include mediation of bacterial stress responses, selective packaging and secretion of virulence determinants, modulation of the host immune response, and contributions to biofilm formation and stability. First characterized as transformasomes in Haemophilus, these membranous blebs facilitate transfer of DNA among bacteria. Nontypeable Haemophilus influenzae (NTHI), an opportunistic pathogen of the upper and lower respiratory tracts, produces OMVs in vivo, but there is a paucity of information regarding both the composition and role of OMVs during NTHI colonization and pathogenesis. We demonstrated that purified NTHI vesicles are 20 to 200 nm in diameter and contain DNA, adhesin P5, IgA endopeptidase, serine protease, and heme utilization protein, suggesting a multifaceted role in virulence. NTHI OMVs can bind to human pharyngeal epithelial cells, resulting in a time- and temperature-dependent aggregation on the host cell surface, with subsequent internalization. OMVs colocalize with the endocytosis protein caveolin, indicating that internalization is mediated by caveolae, which are cholesterol-rich lipid raft domains. Upon interaction with epithelial cells, NTHI OMVs stimulate significant release of the immunomodulatory cytokine interleukin-8 (IL-8) as well as the antimicrobial peptide LL-37. Thus, we demonstrated that NTHI OMVs contain virulence-associated proteins that dynamically interact with and invade host epithelial cells. Beyond their ability to mediate DNA transfer in Haemophilus, OMV stimulation of host immunomodulatory cytokine and antimicrobial peptide release supports a dynamic role for vesiculation in NTHI pathogenesis and clinically relevant disease progression.