RESUMO
A procedure for detection of bacteria destructing cationic surface active substances (CSAS) was developed. The procedure is based on changing colour of pyrocatechin violet, an organic dye from geen-blue in the presence of a CSAS to red-brown in its absence. After application of pyrocatechin violet to bacterial macrocolonies grown on a synthetic medium with a CSAS as the only source of C red-brown zones developed around the colonies which was probably indicative of the CSAS biodestruction. 55 strains of bacteria destructing undecylpyridinium bromide, a CSAS were isolated from water of sewage purification plants. The study of their spectrum with respect to CSASs of different chemical structure revealed 6 bacterial strains capable of destructing all the nine test preparations. It was shown that biodestruction of the CSASs was associated with the long carbon chain and depended on the presence of the substitute in the aromatic ring and branching in the side chain. Therefore, the procedure provides screening of microbes destructing CSASs and may be used for rapid determination of biodestruction of CSASs with different chemical structures.
Assuntos
Detergentes/metabolismo , Compostos de Piridínio/metabolismo , Tensoativos/metabolismo , Microbiologia da Água , Poluentes Químicos da Água , Poluentes da Água , Técnicas Bacteriológicas , Benzenossulfonatos/farmacologia , Biodegradação Ambiental , Meios de Cultura , Técnicas In Vitro , Coloração e Rotulagem/métodosRESUMO
The toxic substance of Erwinia carotovora, isolated in a pure form, is a protein-polysaccharide complex with molecular weight of 90000 daltons. The protein part of the complex contains 17 amino acids with the prevalence of dicarbonic acids and with insignificant quantities of thyrosine and phenylalanine, while the polysaccharide part of the complex contains 6 sugars. The antigenic specificity of the complex is linked with its hydrocarbon part, and the toxicity of the complex is determined by protein. The toxic complex is heat-resistant and most active at the neutral pH value of the culture medium.
