The Impact of Color Cues on the Learning Performance in Video Lectures.

This study explores the learning effects of color cues in video lectures and their underlying mechanisms. With the rapid growth of online education, lifelong learning, and blended learning, video lectures have become integral to teaching and learning. Color, a crucial element in visual design, directs attention, organizes content, and integrates information. Evaluating 78 college students, we assessed learning performance by comparing video content with no-color, single-color, and multi-color cues using eye-tracking technology and cognitive load scales. Results indicate that students viewing videos with color cues demonstrated better retention and transfer test performance, while absence or excess of color cues increased cognitive load. These findings have practical implications for video producers and provide a theoretical foundation for enhancing learners' viewing experience and overall effectiveness. This study not only offers an in-depth analysis of color cue utilization in video lectures, highlighting their positive impact on learning outcomes but also introduces fresh perspectives for educational technology and cognitive psychology research. Future investigations should consider color cue effects in diverse cultural contexts and subject areas, exploring varied strategies to optimize the learning experience.

R-loops act as regulatory switches modulating transcription of COLD-responsive genes in rice.

COLD is a major naturally occurring stress that usually causes complex symptoms and severe yield loss in crops. R-loops function in various cellular processes, including development and stress responses, in plants. However, how R-loops function in COLD responses is largely unknown in COLD susceptible crops like rice (Oryza sativa L.). We conducted DRIP-Seq along with other omics data (RNA-Seq, DNase-Seq and ChIP-Seq) in rice with or without COLD treatment. COLD treatment caused R-loop reprogramming across the genome. COLD-biased R-loops had higher GC content and novel motifs for the binding of distinct transcription factors (TFs). Moreover, R-loops can directly/indirectly modulate the transcription of a subset of COLD-responsive genes, which can be mediated by R-loop overlapping TF-centered or cis-regulatory element-related regulatory networks and lncRNAs, accounting for c. 60% of COLD-induced expression of differential genes in rice, which is different from the findings in Arabidopsis. We validated two R-loop loci with contrasting (negative/positive) roles in the regulation of two individual COLD-responsive gene expression, as potential targets for enhanced COLD resistance. Our study provides detailed evidence showing functions of R-loop reprogramming during COLD responses and provides some potential R-loop loci for genetic and epigenetic manipulation toward breeding of rice varieties with enhanced COLD tolerance.