In vitro assessment of the DNA damage and senility of CD117+ stem cells collected from diabetic mice.

OBJECTIVE: Angiogenesis impairment is a common feature of diabetes mellitus (DM), whereas CD117+ bone marrow cells (BMCs) injury might be responsible for such complication. In this study, we studied the effect of hyperglycemia on the DNA damage and senility of CD117+ bone marrow cells. MATERIALS AND METHODS: We isolated CD117+ BMCs from the Streptozotocin (STZ) induced diabetes and healthy control mice. Oxidative stress was detected by flow cytometric analysis. γ-H2AX, which is the DNA damage mark, was detected by using Western blotting and immunofluorescence histochemistry. We also detected the expression of γ-H2AX and p16 by using Western blotting. RESULTS: Compared with the control mice, the level of reactive oxygen species (ROS) was increased significantly in the CD117+ BMCs collected from the diabetic mice (p<0.05), and the percentage of γ-H2AX positive cells was higher significantly (p<0.01). The expression of γ-H2AX and p16 was increased significantly in the CD117+ BMCs from the diabetic mice. CONCLUSIONS: Our experiments demonstrated the oxidative stress in CD117+ BMCs under DM conditions, while accelerating the DNA damage and senility in CD117+ BMCs as well.

The effects of VDT data entry work on operators.

The before/after study of physiological and biochemical parameters was used to delineate the effects of VDT data entry work on operators. Twenty-nine healthy Chinese students were chosen and divided at random into the simple and the complicated data entry group. The subjects were instructed to work as quickly and correctly as possible according to the 'Data Entry Work Programme' for 150 min. Work performance (correct entry) was automatically recorded once every 10 min. The before/after parameters were tested respectively. The results showed that performance fluctuated over time. It decreased obviously after 50-60 min of work, followed by a rebound, and there was a terminal motivation phenomenon at the end of the test, which was associated with the auto-arousal and cerebral compensatory effort. Changes in physiological parameters revealed that operators were fatigued after data entry work. The adrenaline excretion in urine showed a tendency to increase after simple data entry work. The noradrenaline excretion showed a tendency to decrease after complicated data entry work. The differences in performance, diastolic blood pressure in a standing position and neurobehaviour between two groups indicated that much stress was experienced when performing complicated data entry work.