Fabrication of biconcave discoidal silica capsules and their uptake behavior by smooth muscle cells.

Biconcave discoidal silica microcapsules were fabricated by reaction of tetraethoxysilane (TEOS) on biconcave discoidal Ca(OH)2 templates, followed by core removal. The biconcave discoidal morphology of microcapsules was characterized by confocal laser scanning microscopy (CLSM) and transmission electron microscopy (TEM). The thickness of silica capsule shell can be tuned by two methods, "Gradient concentration" method and "Multi-step growth" method. Through the latter one, the shell thickness can be controlled more effectively. Compared with spherical microcapsules, the biconcave discoidal ones were internalized into smooth muscle cells (SMCs) with a slower rate.

Fabrication of red-blood-cell-like polyelectrolyte microcapsules and their deformation and recovery behavior through a microcapillary.

Multilayer microcapsules with a biconcave discoidal shape mimicking red blood cells (RBCs) are fabricated. The structure of the RBC-like microcapsules is verified by scanning electron and confocal laser scanning microscopies. The capsules show elastic deformation after being forced through a microcapillary with a smaller diameter, exhibiting a high recovery ratio of ≈90%. When the capsules are coated with hemoglobin (Hb),they are able to reversibly bind and release oxygen.

Phenomenon and mechanism of capsule shrinking in alkaline solution containing calcium ions.

Shrinking phenomenon of poly(allylamine hydrochloride) (PAH)/poly(styrene sulfonate, sodium salt) (PSS) multilayer microcapsules was observed when they were incubated in alkaline solutions containing Ca(2+). The shrinking was universal to those polyelectrolyte multilayer capsules regardless of the wall thickness and wall compositions suppose the conditions were proper. The shrinking extent increased along with the increase of solution pH and Ca(2+) concentration, and reached to a maximum value of 70% (from 7.4 to 2.3 µm). The shrunk capsules with a hollow structure and thick wall could well maintain their spherical shape in a dry state. During the capsule shrinking partial loss of the polyelectrolytes especially PSS took place, and the loss amount increased along with the increase of solution pH although the alteration patterns were different at lower Ca(2+) concentration. The complexation of PSS with Ca(2+), which is believed one of the major reasons governing the capsule shrinking, was demonstrated by X-ray photoelectron spectroscopy and turbidity experiment. The mechanism is proposed, which relies on the synergistic effects of deprotonation of PAH and screening of PSS by Ca(2+) leading to the thermodynamically favored-capsule shrinking.

Shape deformation and recovery of multilayer microcapsules after being squeezed through a microchannel.

The deformation and recovery behaviors of multilayer microcapsules were investigated after being forced to flow through a microchannel. The microchannel device with a constriction (5.7 µm in depth) in the middle was designed, and the multilayer microcapsules with different size and layer thickness (and thereby different mechanical strength) were used. Deformation in the microchannel was observed for all the capsules with a size larger than the constriction height, and its extent was mainly governed by the difference between capsule size and constriction height. The squeezed microcapsules could recover their original spherical shape when the deformation extent was smaller than 16%, whereas permanent physical deformation took place when the deformation extent was larger than 34%. The capsules filled with polyelectrolytes could greatly enhance their shape recovery ability due to the higher osmotic pressure in the capsule interior and could well maintain the preloaded low-molecular-weight dyes regardless of the squeezing.