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1.
Sci Data ; 7(1): 402, 2020 11 19.
Artigo em Inglês | MEDLINE | ID: mdl-33214563

RESUMO

The US PulseNet and GenomeTrakr laboratory networks work together within the Genomics for Food Safety (Gen-FS) consortium to collect and analyze genomic data for foodborne pathogen surveillance (species include Salmonella enterica, Listeria monocytogenes, Escherichia coli (STECs), and Campylobactor). In 2017 these two laboratory networks started harmonizing their respective proficiency test exercises, agreeing on distributing a single strain-set and following the same standard operating procedure (SOP) for genomic data collection, running a jointly coordinated annual proficiency test exercise. In this data release we are publishing the reference genomes and raw data submissions for the 2017 and 2018 proficiency test exercises.


Assuntos
Microbiologia de Alimentos/métodos , Inocuidade dos Alimentos , Genômica/normas , Laboratórios/normas , Campylobacter/isolamento & purificação , Escherichia coli/isolamento & purificação , Genoma Bacteriano , Listeria monocytogenes/isolamento & purificação , Salmonella enterica/isolamento & purificação , Estados Unidos
3.
Foodborne Pathog Dis ; 11(4): 301-6, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24484290

RESUMO

Salmonella is an important cause of foodborne illness; however, identifying the source of these infections can be difficult. This is especially true for Salmonella serotype Typhimurium, which is found in diverse agricultural niches. Extended-spectrum cephalosporins (ESC) are one of the primary treatment choices for complicated Salmonella infections. In Salmonella, ESC resistance in the United States is mainly mediated by blaCMY genes carried on various plasmids. In this study, we examined whether the characterization of blaCMY plasmids, along with additional information, can help us identify potential sources of infection by Salmonella, and used serotype Typhimurium as a model. In the United States, monitoring of retail meat, food animals, and ill persons for antimicrobial-resistant Salmonella is conducted by the National Antimicrobial Resistance Monitoring System. In 2008, 70 isolates (70/581; 12.0%) (34 isolates from retail meat, 23 food animal, and 13 human) were resistant to ceftriaxone and amoxicillin/clavulanic acid. All were polymerase chain reaction (PCR)-positive for blaCMY and 59/70 (84.3%) of these genes were plasmid encoded. PCR-based replicon typing identified 42/59 (71.2%) IncI1-blaCMY plasmids and 17/59 (28.8%) IncA/C-blaCMY plasmids. Isolates from chickens or chicken products with blaCMY plasmids primarily had IncI1-blaCMY plasmids (37/40; 92.5%), while all isolates from cattle had IncA/C-blaCMY plasmids. Isolates from humans had either IncA/C- blaCMY (n=8/12; [66.7%]) or IncI1- blaCMY (n=4/12 [33.3%]) plasmids. All of the IncI1-blaCMY plasmids were ST12 or were closely related to ST12. Antimicrobial susceptibility patterns (AST) and pulsed-field gel electrophoresis (PFGE) patterns of the isolates were also compared and differences were identified between isolate sources. When the source of a Typhimurium outbreak or sporadic illness is unknown, characterizing the outbreak isolate's blaCMY plasmids, AST, and PFGE patterns may help identify it.


Assuntos
Anti-Infecciosos/farmacologia , Doenças Transmitidas por Alimentos/microbiologia , Carne/microbiologia , Infecções por Salmonella/microbiologia , Salmonella typhimurium/genética , beta-Lactamases/genética , Amoxicilina/farmacologia , Animais , Bovinos , Ceftriaxona/farmacologia , Galinhas , Ácido Clavulânico/farmacologia , Eletroforese em Gel de Campo Pulsado , Genótipo , Humanos , Plasmídeos/genética , Salmonella typhimurium/efeitos dos fármacos , Salmonella typhimurium/enzimologia , Análise de Sequência de DNA , Estados Unidos , Resistência beta-Lactâmica/genética
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