Immunostimulatory effects of dietary verjuice (Vitis vinifera) on immune response and transcription of immune-related genes in juvenile rainbow trout (Oncorhynchusmykiss).

The development of green aquaculture practices has led to the supplementation of fish diets with natural immunostimulants such as organic acids. This study aimed to assess the dietary effects of verjuice (VJ; unfermented unripe grapes; Vitis vinifera) on hematological parameters, skin mucosal immunity, transcriptional immune responses, and antibacterial serum activity against Aeromonas hydrophila in rainbow trout. The fish (51.0 ± 2.4 g) were randomly distributed into 15 tanks and fed ad-libitum thrice daily with diets containing different levels of VJ including 0 (control; VJ-0), 3 (VJ-3), 6 (VJ-6), 9 (VJ-9), and 12 (VJ-12) mL/kg VJ for 56 d. Results showed that immuno-hematological parameters (total white blood cells, neutrophils, and monocytes) were improved in VJ-added groups (P < 0.05). In addition, dietary VJ (9 mL/kg) modulated serum immunological parameters. Skin mucus immunology exhibited a notable increase in alkaline phosphatase, lysozyme activity, alkaline protease, total protein, total immunoglobulin, and esterase levels in VJ-9 group compared with those in the control group (P < 0.05). The mRNA expression of interleukin-1ß, interleukin-6, interleukin-8, and immunoglobulin M were significantly higher in VJ-9 group than in the control (P < 0.05). Furthermore, the results of the antibacterial evaluation showed that A. hydrophila growth was significantly inhibited in the serum samples from VJ-3 to VJ-9 groups after the 56th day and in all VJ-treated groups after the 70th (P < 0.05). In conclusion, dietary VJ is a novel immunostimulant and the optimal dietary supplementation level of 6.65-7.46 mL/kg can effectively improve immune responses in rainbow trout.

Trace elements in the muscle and liver tissues of Garra shamal from the freshwater ecosystem of Oman: an exposure risk assessment.

Anthropogenic activities lead to environmental contamination with foreign substances such as heavy metals. This work was aimed to monitor trace elements (total arsenic (As), cadmium (Cd), chrome (Cr), cobalt (Co), copper (Cu), lead (Pb), manganese (Mn), mercury (Hg), nickel (Ni), and zinc (Zn)) contamination levels (dry weight base) in three natural freshwater reservoirs of Oman including Al Khawd and Al Amarat (Muscat Governorate) and Surur area (Ad Dakhiliyah Governorate as control area) using a native benthic inland fish (Garra shamal; Cyprinidae) for the first time. The muscle and liver of a hundred and twenty G. shamal were collected to assess the degree of metal contamination. Atomic absorption spectrometry was used as an analytical technique. From the spectrum of analyzed elements, we found Zn as a major element in monitored areas. The statistically significant (P < 0.05) highest concentrations of Zn liver (0.275 ± 0.065 µg/g) were in Al Amarat compared to the other areas. The concentrations of monitored elements in the fish muscle were lower than the liver samples. Furthermore, the fish length was significantly correlated with the accumulation of Hg and Co in both muscle and liver samples. In all analyzed fish from Oman inland water, the concentrations of elements were below the permissible limits; however, additional research is needed.

Nucleotides supplementation (Nucleoforce fish™) in Caspian roach (Rutilus caspicus) diet: Growth performance, skin mucosal immune response, and resistance to salinity stress.

In this study, the growth, epidermal mucosal immunity, expression of growth-related genes, cross-protection, and resistance to salinity stress of Caspian roach were scrutinized in response to dietary levels of nucleotides (NT). Accordingly, 1200 fish (0.51 ± 0.01 g) were fed ad libitum with a basal diet (38.88 % crude protein and 10.04 % crude lipid in dry basis) containing incremental levels of NT at 0 (NT-0; control), 0.3 g kg-1 (NT-0.3), 0.6 g kg-1 (NT-0.6), and 1.2 g kg-1 (NT-1.2) for 8 weeks in triplicates. The growth performance was significantly increased in the fish fed with NT-0.6 and NT-1.2 diets compared to the control group (p < 0.05). A significant elevation in the growth hormone and insulin-like growth factor-I gene expression was recorded in NT-added groups at 0.6 and 1.2 g kg-1 compared to the control group (p < 0.05). In contrast to the control group, feeding on NT-0.6 and NT-1.2 diets had a remarkable effect on the skin mucus soluble protein and immunoglobulin levels (p < 0.05). After the feeding trial, we examined how salinity stress (15 g/l salinity) lonely and salinity stress under non-lethal thermal shock (+10 °C) affected heat shock protein (HSP70). Then, the mRNA expression of HSP70 gene from the gill was analyzed at 0, 2, 8, and 24 h post-challenge tests. The HSP70 gene expression level was approximately up-regulated more than 2-fold in NT-6 and NT-1.2 treatments compared to the control group under the salinity stress. Altogether, this research represents that the addition of NT at 0.6 and 1.2 g kg-1 in Caspian roach diet can improve overall performance and resistance to salinity stress.

Characterization of novel genotyping-by-sequencing (GBS)-based simple sequence repeats (SSRs) and their application for population genomics of Capoeta aculeata (Valenciennes, 1844).

BACKGROUND: The species Capoeta aculeata (Valenciennes, 1844) is one of the most important freshwater species endemic to Iran. However, the investigation of the population genetic structure of this species is limited by the low number of molecular markers currently described. METHODS AND RESULTS: In this study, we implemented next generation sequencing technology to identify polymorphic microsatellite markers and investigate the population genetic structure of C. aculeata sampled from three geographical sites in Iran. We characterized and developed 36 novel polymorphic microsatellite markers and these loci were examined in 120 individuals from three populations occurring in the Zagros basin. The average number of alleles per locus varied from 1.7 to 16 (average = 7.89). The results showed that, the polymorphism information content (PIC) of these simple sequence repeat (SSR) loci varied from 0.254 to 0.888. The observed heterozygosity (HO) per locus ranged from 0.170 to 0.881, while the expected heterozygosity (HE) per locus was from 0.170 to 0.881. Among these SSR loci, 20 loci deviated significantly from the Hardy-Weinberg equilibrium after Bonferroni correction (p < 0.05). CONCLUSIONS: These microsatellite markers could provide a valuable tool for future population and conservation genetics studies of C. aculeate and other closely related species.

Dietary Gracilaria persica mediated the growth performance, fillet colouration, and immune response of Persian sturgeon (Acipenser persicus).

Algal seaweeds have abundant amounts of active substances and can be used as pharmaceuticals and biomedicals in aquafeeds. In this context, the powder of red macroalgae Gracilaria persica was included in the diets of Persian sturgeon at the rate of 0, 2.5, 5, and 10 g/kg to investigate its role on the growth rate, fillet colouration, haemato-biochemical indices, serum, and skin mucus immunity. The weight gain, SGR, and FCR displayed no significant changes in fish fed varying levels of G. persica (P > 0.05). The level of total carotenoids was significantly higher in the blood and fillet of fish fed 5 and 10 g G. persica/kg diet (P < 0.05). Dietary G. persica significantly altered RBCs, WBCs, and HCT at 5, and 10 g/kg, whereas the Hb was increased in fish fed 5 g/kg (P < 0.05). The blood total protein and albumin were significantly increased in fish fed 5 and 10 g/kg (P < 0.05). No significant alterations were observed on ALT, AST, ALP, and glucose levels of fish fed varying levels of G. persica (P > 0.05). Serum Ig, lysozyme, superoxide dismutase, catalase, and respiratory burst activities were increased in fish fed 5, and 10 g/kg than fish fed 0 and 2.5 g/kg diet (P < 0.05). The level of total protein and lysozyme activity in the skin mucus were significantly higher in the blood and fillet of fish fed 5, and 10 g G. persica/kg diet than fish fed 0 and 2.5 g/kg (P < 0.05). Based on the obtained results, G. persica can be used as a feasible feed additive in the diets of Persian sturgeon at 5-10 g/kg diet.

The potential use of stickwater from a kilka fishmeal plant in Dunaliella salina cultivation.

In this study, the possibility of culturing Dunaliella salina in stickwater (SW) as the main effluent of fishmeal plants was evaluated. D. salina was grown in different media obtained by replacing standard Guillard medium (F/2) with SW at 0% (control), 10%, 25%, 50%, 75%, and 100% ratios. The cell density, pigment contents, proximate composition, saponification value, and fatty acids (FAs) profiles were measured for 14 days. SW was collected from a kilka fishmeal factory in northern Iran, and the characteristics indicated high concentrations of nitrate (242.00 mg L-1) and phosphate (11.13 mg L-1). A significant increase in the cell density was observed in 14 days when 75% SW was used. Moreover, SW significantly affected the pigment contents. The highest contents of chlorophylls, total carotenoids, and ß-carotene (3.64 µg mL-1) were calculated in 75% SW. According to the algal proximate composition, the highest and lowest contents of lipid were accumulated in 75% and 100% SW, respectively (p < 0.05). The highest level of saturated FAs was observed in 75% SW compared with the others (p < 0.05). In conclusion, replacing F/2 with SW indicated the capability of D. salina to grow in a treated medium with 75% SW substitution as a bioremediator.

Dietary Application of the Probiotic Lactobacillus plantarum 426951 Enhances Immune Status and Growth of Rainbow Trout (Oncorhynchus mykiss) Vaccinated Against Yersinia ruckeri.

This study was aimed to assess the effect of oral application of Lactobacillus plantarum (2 × 107 CFU g-1 feed) as a probiotic on growth performance and immune status of vaccinated rainbow trout (29.5 ± 2 g) to yersiniosis at 16 ± 2 °C for 72 days. Fish were randomly allocated into 12 fiber glass tanks (4100 L) at a density of 80 fish per tank (240 fish per treatment). The results revealed that the activity of lysozyme and alkaline phosphatase was significantly higher in immunized fish fed with diet supplemented with probiotic (vaccine +probiotic) than that in the immunized group fed with basal diet (vaccine group) while no significant differences in levels of hematological parameters, complements, total IgM, proteins, and the intestine lactic acid bacteria (LAB) were detected. Also, significantly a better growth performance in terms of feed conversion ratio, weight gain, and thermal growth coefficient was seen in the vaccine + probiotic group than that in the vaccine group. These results indicate that feeding probiotic after vaccination can enhance the efficacy of immersion vaccination to Yersinia ruckeri.

Some immunological responses of common carp (Cyprinus carpio) fingerling to acute extremely low-frequency electromagnetic fields (50 Hz).

Increasing of anthropogenic electromagnetic fields in aquatic environments has been recently become the core of attention. In this study, the effect of extremely low-frequency electromagnetic fields (50 Hz) on immune status and metabolic markers of common carp fingerling was assessed. The fish were exposed to extremely low-frequency electromagnetic fields at four intensities of 0.1, 0.5, 1, and 2 mT only once for 2 h; then, they were reared for 60 days. Results showed that the levels of aspartate aminotransferase (AST) and alanine transaminase (ALT) and alkaline phosphatase (ALP) were increased with an increase in the electromagnetic field intensity on 15 and 60 days post exposure. A significant increase was obtained in these enzyme levels in all the tested intensities compared to the control one (p < 0.05), with a maximum value measured in 2-mT trail. Conversely, with an increasing in the electromagnetic intensity, the levels of C3, C4, and lysozyme were reduced in all the treated groups in comparison with the control group (p < 0.05). The results suggested a significant impact of electromagnetic on fish immunophysiological functions. Therefore, it is required to have serious attention in aquatic ecosystems.

Biological nutrient recovery from culturing of pearl gourami (Trichogaster leerii ) by cherry tomato (Solanum lycopersicum) in aquaponic system.

The possibility of using different densities of cherry tomato as a bio-filter in a simple media-based aquaponic system to recycle nutrients from pearl gourami intensive culture wastewater was evaluated. Water quality parameters including total ammonia nitrogen (TAN), nitrite (NO2-), nitrate (NO3-), phosphate (PO43-), pH, and dissolved oxygen (DO) were determined in outlet of the aquaponic system during a 60-day experimental period. Cherry tomato was planted at four densities of 0 (control), 3 (T1), 6 (T2), and 9 (T3) plants per aquaponic unit with a constant fish stock density. Each treatment was equipped with aquaponic systems containing fish tank and plant growing bed. Productivity of the system was measured by recording the fish and plant growth indices. The potential in removing nitrogen of the water was the highest in T3 (with nine plants) compared to other treatments (p < 0.05). The highest concentrations of TAN (6.59 ± 0.241 mg/L), nitrite (0.42 ± 0.005 mg/L), nitrate (0.45 ± 0.162 mg/L), and phosphate (30.47 ± 0.371 mg/L) were obtained in control group, while the lowest concentrations of TAN (0.05 ± 0.091 mg/L), NO2- (0.11 ± 0.008 mg/L), NO3- (29.77 ± 0.205 mg/L), and phosphate (18.59 ± 0.185 mg/L) were detected in T3 (p < 0.05). The maximum fish weight gain was recorded in T3 (26 ± 0.014%) with 1.26 ± 0.059 FCR, and the lowest fish weight gain was measured in the control group (15 ± 0.024%) with 2.19 ± 0.446 FCR (p < 0.05). Total plant length gain was reached at the maximum value in T3 (74.70 ± 1.153 cm) in comparison to other groups (p < 0.05). It was concluded that small-scale aquaponic growing bed system can be created a sustainable ecosystem which both the plant and fish can thrive and suitable for home-made production system.