Serum IgG-like glycoferroprotein: identification of its final dissociation form of thermostable protein coupled with albumin.

Human serum IgG-like glycoferroprotein identical to ascitic IgG-like glycoferroprotein that binds labeled monoclonal antibodies to CA125 is a complex consisting of three proteins: IgG, human serum albumin, and unidentified thermostable protein. Final dissociation form of serum IgG-like glycoferroprotein also appears as a complex of three nonidentical polypeptides with a molecular weight of 55 kDa (PC55) migrating in the albumin zone of thermostable protein coupled with albumin and structures chemically identical to human serum albumin and IgG heavy chains. Under denaturing conditions of electrophoresis in polyacrylamide gel, IgG-like glycoferroprotein and PC55 have the same molecular weight (about 55 kDa), while under reducing conditions their weight is about 75 kDa. Transition form (form the lower to the higher molecular weight) appears as an oblique (at about ≈ 30°) protein band creating a ladder string effect. Ladder string effect was reproduced with thermostable protein coupled with albumin, PC55, IgG-like glycoferroprotein, with all commercially available human and bovine albumins, rat albumin as well as with heated and renatured albumins and can serve as electrophoretic identification sign for thermostable protein coupled with albumin. Renatured after boiling (100°C for 15 min) bovine albumin under reducing conditions appeared as bow string twisted in helix, that raises molecule in 2.5 turns from ≈ 2 to ≈ 75 kDa. These data attest to the existence of an albumin double and to its possible double structure.

[Results of surgical and radiotherapy of prostatic cancer T1-4N0-1M0].

To compare the results of radical prostatectomy and conformal radiotherapy in prostatic cancer T1-4N0-1M0, we made a retrospective study of 306 patients with prostatic cancer T1-4N0-1M0 of whom 144 (47.1%) were treated surgically (radical prostatectomy) while 162 (52.9%) were exposed to extracorporeal conformic radiotherapy. Follow-up median was 30.7 +/- 29.8 months. Five and 10-year overall, specific and PSA recurrence free survival in 306 patients was 94.0% and 90.1% (median was not achieved), 96.6% and 94.3% (median was not achieved), 66.1 and 49.2% (median was 84.0 +/- 4.4 months). In multifactorial analysis significant prognostic factors of PSA recurrence free survival were T category (p = 0.021) and Glison's sum (p = 0.002). In the subgroup of patients with local prostatic cancer there was a significant superiority of the operated patients by PSA recurrence free survival over irradiated group in baseline PSA < 10 ng/ ml (p = 0.015), Glison's index < 7 (p = 0.071) and combination of these factors (p = 0.018). A favourable prognosis factor of PSA recurrence free survival in operated patients was operative Glison's index < 7 (p = 0.001), among operated patients--nadir PSA < 1 ng/ ml (p = 0.003). Surgical and radiation treatment of local and locally advanced prostatic cancer provided satisfactory results. In the group of good prognosis (cT1-2N0, PSA < 10 ng/ml, Glison's sum < 7) radical prostatectomy gives advantage of PSA recurrence free survival. In patients with prostatic cancer cT > T2, N+, Glison's index > 7 and PSA > 10 ng/ml surgical treatment and remote radiotherapy are equally effective in respect to survival free of biochemical recurrence.

[Antibodies to stuctural proteins of endogenous retrovirus of the HERV-K/HTDV family as markers of human germ cell tumors].

Human germ cell tumors (GCT) have been found to be closely associated with the expression of HERV-K/HTDV proviruses and most patients with GCT produce antibodies to the major HERV-K/HTDV Gag and Env proteins. The findings have shown a strong association of the level of HERV-K/HTDV antibodies with the clinical course of the disease and therapy success, which makes it possible to confirm the fact that viral protein antibodies may be used as an additional marker of GCT.

[Results of a 5-year screening for prostatic cancer]. / Itogi 5-letnego skrininga raka predstatel'noi zhelezy.

The results of 5-year screening (1996-2000) for prostatic cancer in 1129 males 40 to 80 years of age are presented. The examination included: measurement of blood levels of prostate-specific antigen (PSA), finger rectal examination, transrectal ultrasonic examination (TRUE) and, on demand, biopsy of the prostatic gland. Prostatic cancer was diagnosed in 1.5, 2.2 and 16% patients having PSA levels of 0-4.0, 4.0-10.0 and 10.0-30.0% ng/ml, respectively. At finger rectal examination prostatic cancer was suspected in 8% examinees, only in 33% of them the diagnosis was verified morphologically. By TRUE evidence 7% examinees were suspected and in 44.3% of them prostatic cancer was confirmed. Thus, biopsy proved necessary in 172 cases of 1129 examinees. In 64 (5.7%) males prostatic cancer was diagnosed and confirmed. Early prostatic cancer in the screened men and those consulted in the outpatient department of the National Cancer Research Center was detected in 77.7 and 22% men, respectively. The conclusion is made that men over 50 years of age should undergo prophylactic examination of the prostatic gland once a year.

[The use of modified ultrafiltration in correcting complex congenital heart defects in newborn and nursing infants]. / Primenenie modifitsirovannoi ul'trafil'tratsii pri korrektsii slozhnykh vrozhdennykh porokov serdtsa u novorozhdennykh i grudnykh detei.

Modified ultrafiltration (MUF) was used in radical correction of complex congenital heart disease in 61 newborns and infants. The children were divided into 2 groups: group 1, 46 patients subjected to MUF, and group 2, 15 children operated on without MUF. Hemodynamic, hematological, biochemical, and immunological parameters were monitored. MUF in the newborns and infants helped adequately correct the hemohydrobalance due to ultrafiltration in the patient and hemoconcentration of the remaining volume in artificial ventilation device; it normalized the hematocrit values without additional infusion of donor blood. Elimination of inflammation mediators and cytokines recommends MUF for the treatment of newborns and infants.

[The modified effect of carotenoids on rat forestomach carcinogenesis, induced by N-methyl-N'-nitro-N-nitrosoguanidine]. / Modifitsiruiushchii éffekt karotinoidov na kantserogenez predzheludka u krys, indutsirovannykh N-metil-N'-nitro-N-nitrozoguanidinom.

A modifying effect of carotenoids on carcinogenesis induced by N-methyl-H'-nitro-N-nitrosoguanidine (MNNG) in the rumen was studied in four groups of rats weighing 100-120 g. A diet of the first group contained a highly carotenoid complex from wild rose fruits at a dose of 30 mg/kg 3 times weekly; MNNG was administered at a dose of 5 mg per animal by means of a gastric tube for two days. Administration of the drug was repeated within 5 days. Treatment with the carotenoid complex was carried out 2 months before the MNNG administration. Group 2 and 3 received only MNNG or the carotenoid complex, respectively; Group 4 served as intact control. Tumors were detected in 33 rats of 81 animals studied (40.7%) in Group 1 and in 53 rats of 74 animals (71.6%) of Group 2 (P < 0.01). The mean time of the tumors detection constituted 40.7 +/- 9.5 days in Group I and 68.7 +/- 0.3 days (P < 0.05) in Group 2. All the tumors detected were localized in the rumen and consisted of papillomas appeared mainly as multiple forms. The data obtained suggest that carotenoids exhibited the modifying effect on MNNG-induced carcinogenesis of the rat rumen.

[The effect of lipochromin on spontaneous carcinogenesis in mice]. / Vliianie preparata lipokhromina na spontannyi kantserogenez myshei.

Development of spontaneous hepatomas was studied in CBA mice, which were maintained on a diet containing the carotenoid drug lipochromine in a dose of 25 mg/kg during their life beginning at the age of 3 months twice a week. Concentrations of vitamin A (as retinol) and beta-carotene were estimated in mice blood and liver tissue by means of HPLC. Decrease in occurrence of spontaneous tumors in liver tissue as well as distinct increase in periods of their development were detected in the animals treated with the drug. The phenomenon observed correlated positively with an increase in concentration of beta-carotene in liver tissue of tumor-bearing mice, thus indicating the preventive effect of lipochromine on spontaneous carcinogenesis in mice CBA strain.