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1.
Zhongguo Zhong Yao Za Zhi ; 43(4): 672-675, 2018 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-29600639

RESUMO

As traditional Chinese medicinal herbs, Physalis plants have a variety of pharmacological activities, such as anti-inflammatory, anti-oxidant, and anti-cancer effects, and have been used for the treatment of malaria, rheumatism, hepatitis, asthma, and cancer. In addition to the medicinal value, many Physalis species are also the high-grade nutrition health care fruits, can be made canned and candied etc. In the study, the application progress of DNA molecular marker technologies in medicinal Physalis plants in recent years was reviewed, in order to provide an important molecular technical basis for the identification, classification and rational development and protection of medicinal Physalis resources.


Assuntos
DNA de Plantas/genética , Marcadores Genéticos , Physalis/genética , Plantas Medicinais/genética
2.
Sci Rep ; 7(1): 1873, 2017 05 12.
Artigo em Inglês | MEDLINE | ID: mdl-28500349

RESUMO

Lysine succinylation is a novel dynamic and evolutionarily conserved post-translational modification (PTM) that regulates various biological processes. 'Anji Baicha' is an albino tea variety that exhibits temperature-based variability of leaf colour and amino acid concentrations. However, the mechanism underlying albinism in 'Anji Baicha' has not been investigated at the level of succinylation. Here, we identify 3530 lysine succinylation sites mapped to 2132 proteins in 'Anji Baicha', representing the first extensive data on the lysine succinylome in the tea plant. Eleven conserved succinylation motifs were enriched among the identified succinylated peptides. The protein-protein interaction maps were visualized using Cytoscape software. Comparison across three typical developmental stages of 'Anji Baicha' revealed that proteins exhibiting differential succinylation levels were primarily involved in photosynthesis, carbon fixation, biosynthesis of amino acids and porphyrin and chlorophyll metabolism, suggesting that these succinylated proteins are involved in 'Anji Baicha' leaf colour variability. These results not only deepen our understanding of the mechanism underlying 'Anji Baicha' albinism and the regulatory role of succinylation in the tea plant but also provide new insight into molecular breeding for leaf colour variety.


Assuntos
Albinismo/metabolismo , Camellia sinensis/metabolismo , Proteoma , Proteômica , Motivos de Aminoácidos , Sequência de Aminoácidos , Camellia sinensis/genética , Biologia Computacional/métodos , Perfilação da Expressão Gênica , Lisina/química , Anotação de Sequência Molecular , Fenótipo , Desenvolvimento Vegetal/genética , Folhas de Planta/metabolismo , Proteínas de Plantas/química , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Mapeamento de Interação de Proteínas , Mapas de Interação de Proteínas , Processamento de Proteína Pós-Traducional , Proteômica/métodos
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